• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Elucidation of Inhibitory Mechanisms of Mouse Bone Formation by Endocrine Disrupting Chemicals.

Research Project

Project/Area Number 15510045
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Risk sciences of radiation/Chemicals
Research InstitutionToin University of Yokohama (2004)
Tokyo Institute of Technology (2003)

Principal Investigator

HAGIWARA Hiromi  Toin University of Yokohama, Faculty of Biomedical Engineering, Professor, 医用工学部, 教授 (90189465)

Co-Investigator(Kenkyū-buntansha) ISHIHARA Yoko  Toin University of Yokohama, 医学部, 助教授 (50203021)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2004: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2003: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsEndocrine disrupting chemicals / Methylcholanthren / Tributyltin / Arkylphenol / Bone metabolism / Osteoblasts / Osteoclasts / Inhibition of bone formation / 植物ポリフェノール / 阻害メカニズム / ダイオキシン / 軟骨細胞
Research Abstract

(1)3-Methycholanthren (3MC), a arylhydrocarbon receptor ligand, inhibited the calcification of bone in fetal mouse. 3MC also inhibited the formation of osteoclasts and the proliferation and differentiation of osteoblasts.
(2)We found that tributyltin treatment induced delay of ossification of calvariae in fetal mouse. Tributyltin also inhibited the formation of osteoclasts and the proliferation and differentiation of osteoblasts. The aromatase activity was not involved in those inhibitory processes.
(3)We examined effects of alkylphenols such as nonylphenol and octylphenol on bone metabolisms. These compounds did not affect the proliferation and differentiation of osteoblasts. However, the formation of osteoclasts was inhibited by alkylphenols.
(4)We examined effects of a phytopolyphenol, quercetin, on bone metabolisms. Quercetin inhibited the formation of osteoclasts and the proliferation and differentiation of osteoblasts through the stimulation of expression of p21protein.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (16 results)

All 2004 2003 Other

All Journal Article (13 results) Publications (3 results)

  • [Journal Article] Inhibition of osteoclast formation by 3-methlcholanthrene, a ligand for arylhydrocarbon receptor : suppression of osteoclast differentiation factor in osteogenic cells.2004

    • Author(s)
      M.Naruse, E.Otsuka, M.Naruse, Y.Ishihara, S.Tomita, H.Hagiwara
    • Journal Title

      Biochemical Pharmacology 67

      Pages: 119-127

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Ouercetin, a flavonoid, inhibits the proliferation, differentiation, and mineralization of osteoblasts in vitro.2004

    • Author(s)
      M.Notaya, Y.Tsukamoto, H.Nishimura, J-T.Woo, K.Nagai, I-S.Lee, H.Hagiwara
    • Journal Title

      European Journal of Pharmacology 485

      Pages: 89-96

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Inhibition of ossification in vivo and differentiation of osteoclasts in vitro by tributyltin.2004

    • Author(s)
      Y.Tsukamoto, Y.Ishihara, S.Tomita, H.Hagiwara
    • Journal Title

      Biochemical Pharmacology 68

      Pages: 739-746

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Quercetin suppresses bone resorption by inhibiting the differentiation and activation of osteoclasts.2004

    • Author(s)
      J-T.Woo, H.Nakamura, M, Notoya, T.Yonezawa, N.Udagawa, I-S.Lee, M.Ohnishi, H.Hagiwara, K.Nagai
    • Journal Title

      Biol. Pharm. Bull. 27

      Pages: 504-509

    • NAID

      110003608794

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Runnx-2 is not essential for the vitamin D-regulated expression of RANKL and osteoprotegerin in osteoblastic cells.2004

    • Author(s)
      M.Notoya, E.Otsuka, A.Yamaguchi, H.Hagiwara
    • Journal Title

      Biochemical and Biophysical Research Communications 324

      Pages: 655-660

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Inhibition of osteoclast formation by 3-methyl-cholanthrene, a ligand for arylhydrocarbon receptor : suppression of osteoclast differentiation factor in osteogenic cells.2004

    • Author(s)
      M.Naruse, E.Otsuka, M.Naruse, Y.Ishihara, S.Tomita, H.Hagiwara
    • Journal Title

      Biochemical Pharmacology 67

      Pages: 119-127

    • NAID

      40006236476

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Quercetin, a flavonoid, inhibits the proliferation, differentiation, and mineralization of osteoblasts in vitro.2004

    • Author(s)
      M.Notoya, Y, Tsukamoto, H.Nishimura, J-T.Woo, K.Nagai, I-S.Lee, H.Hagiwara
    • Journal Title

      European Journal of Pharmacology 485

      Pages: 89-96

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Inhibition of ossification in vivo and differentiation of osteoblasts in vitro by tributyltin.2004

    • Author(s)
      Y.Tsukamoto, Y.Ishihara, S.Tomita, H.Hagiwara
    • Journal Title

      Biochemical Pharmacology 68

      Pages: 739-746

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Quercetin suppresses bone resorption by inhibiting the differentiation and activation of osteoclasts.2004

    • Author(s)
      J-T.Woo, H.Nakamura, M.Notoya, T.Yonezawa, N.Udagawa, I-S.Lee, M.Ohnishi, H.Hagiwara, K.Nagai
    • Journal Title

      Biol.Pharm.Bull. 27

      Pages: 504-509

    • NAID

      110003608794

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Runx-2 is not essential for the vitamin D-regulated expression of RANKL and osteoprotegerin in osteoblastic cells.2004

    • Author(s)
      M.Notoya, E.Otsuka, A.Yamaguchi, H.Hagiwara
    • Journal Title

      Biochemical and Biophysical Research Communications 324

      Pages: 655-660

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Inhibition of ossification in vivo and differentiation of osteoblasts in vitro by tributyltin.2004

    • Author(s)
      Y.Tsukamoto, Y.Ishihara, S.Tomita, H.Hagiwara
    • Journal Title

      Biochmical Pharmacology 68

      Pages: 739-746

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Quercetin suppresses bone resorption by inhibiting the differentiation and activation of osteoclasis.2004

    • Author(s)
      J-T.Woo, H.Nakamura, M.Notoya T.Yonezawa, N.Udagawa, I-S.Lee, M.Ohnishi, H.Hagiwara, K.Nagai
    • Journal Title

      Biol. Pharm. Bull. 27・4

      Pages: 504-509

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Treatment of myoblastic C2C12 cells with BMP-2 stimulates vitamin D-induced formation of osteoclasts.2003

    • Author(s)
      E.Otsuka, M.Notoya, H.Hagiwara
    • Journal Title

      Calcified Tissue International 73

      Pages: 72-77

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] E.Otsuka, M.Notoya, H.Hagiwara: "Treatment of myoblastic C2C12 cells with BMP-2 stimulates vitamin D-induced formation of osteoclasts."Calcified Tissue International. 73. 72-77 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] M.Naruse, E.Otsuka, M.Naruse, Y.Ishihara, S.Tomita, H.Hagiwara: "Inhibition of osteoclast formation by 3-methylcholanthrene, a ligand for arylhydrocarbon receptor : suppression of osteoclast differentiation factor in osteogenic cells."Biochemical Pharmacology. 67. 119-127 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] M.Notoya, Y.Tsukamoto, H.Nishimura, JT.Woo, K.Nagai, IS.Lee, H.Hagiwara: "Quercetin, a flavonoid, inhibits the proliferation, differentiation, and mineralization of osteoblasts in vitro."European Journal of Pharmacology. 485. 89-96 (2004)

    • Related Report
      2003 Annual Research Report

URL: 

Published: 2003-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi