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Brain neurotransmitter abnormalities and behavioral alterations induced by in utero exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin at secondary sexual characteristic stage of development.

Research Project

Project/Area Number 15510062
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Risk sciences of radiation/Chemicals
Research InstitutionKagoshima Immaculate Heart University

Principal Investigator

KUCHIIWA Toshiko  Kagoshima Immaculate Heart University, Faculty of International Human Sciences, professor, 国際人間学部, 教授 (00270158)

Co-Investigator(Kenkyū-buntansha) KUCHIIWA Satoshi  Kagoshima University, Graduate School of Medical and Dental Sciences, Associate Professor, 大学院医歯学総合研究科, 助教授 (90161637)
Project Period (FY) 2003 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2006: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2005: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2004: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2003: ¥1,600,000 (Direct Cost: ¥1,600,000)
Keywordsdioxin toxicity / noradrenaline / immunohistochemistry / neurotransmitter disorder / endocrine disruptor / depression / encephalopathy / behavioral abnormalities / 脳影響 / 環境ホルモン / 神経伝達物質 / TCDD / 第二次性徴 / 胎盤・母乳暴露 / ウェスタンブロット法 / ノルエピネフリン / 内分泌かく乱化学物質 / セロトニン / エンケファリン / 一酸化窒素 / 環境かく乱化学物質
Research Abstract

1. We observed that perinatal exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) caused enhanced hyperactive motor responses to soft touch simulation on the skin. We developed a new research instrument, Touch Response Meter, computing motor response activity of laboratory animals. The socially isolated depression model mice and the dioxin exposed depressed mice were tested in this instrument. We demonstrated that the screening of the depressed animals and quantitative estimating of degree of depression were computable using this new research instrument.
2. Female ddY mice were administered TCDD by gavage and mated with normal male mice. The nose poke test was performed in their offspring to evaluate the effects of perinatal TCDD exposure on exploratory behavior at the secondary sexual characteristic and pubertal stages of development. The perinatal TCDD-exposed offspring at these stages showed decreased nose poke behavior, suggesting the perinatal TCDD exposure decrease exploratory behavior in these stages.
3. Female pregnant mice were received oral administration of TCDD to evaluate the behavioral abnormalities of their offspring at the secondary sexual characteristic and pubertal stages of development. The male offspring showed hyperactive motor responses such as 'kicking off', 'escaping', 'attacking' and 'hiding' to mechanical soft stimulation on their skin. The behaviors continued from postnatal week 8 to 12 and more.
4. Female pregnant mice were administered TCDD by gavage to evaluate the effect of perinatal TCDD exposure on the activity of the locus coeruleus of their offspring at the pubertal and young adult stages. A small number of immunopositive c-Fos neurons were observed in the nucleus in the high dosed TCDD-exposed offspring, but few positive cells were observed in the normal and low dosed offspring, indicating that the perinatal exposure of TCDD does not effect on the activity of the locus coeruleus of the male offspring at these stages.

Report

(5 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (10 results)

All 2008 2007 2006 Other

All Journal Article (4 results) Patent(Industrial Property Rights) (4 results) Publications (2 results)

  • [Journal Article] 実験動物における機械的接触刺激に対する応答行動の定量的計測-鬱動物の検出と鬱症状定量の試み-2008

    • Author(s)
      口岩俊子
    • Journal Title

      鹿児島純心女子大学大学院人間科学研究科紀要 第3号(印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] ダイオキシン胎盤・母乳暴露により第二次性徴期から若年期にかけてマウスに発症する接触刺激に対する過敏な応答行動-ダイオキシン周産期暴露に起因する若年期鬱発症の可能性について-2008

    • Author(s)
      口岩俊子
    • Journal Title

      鹿児島純心女子大学大学院人間科学研究科紀要 第3号(印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A new quantitative method to estimate behavioral hyperactive responses to mechanical soft touch : Attempts of screening of depressed animals and quantitaion of symptoms of depression.2008

    • Author(s)
      Toshiko Kuchiiwa
    • Journal Title

      International Human Studies vol.13

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 実験動物における機械的接触刺激に対する応答行動の定量的計測 -欝動物の検出と鬱症状定量の試み-2008

    • Author(s)
      口岩俊子
    • Journal Title

      鹿児島純心大学国際人間学部研究紀要 13号(印刷中)

    • Related Report
      2006 Annual Research Report
  • [Patent(Industrial Property Rights)] 刺激応答計測装置および刺激応答計測法2007

    • Inventor(s)
      口岩 聡, 口岩俊子
    • Industrial Property Rights Holder
      鹿児島大学
    • Industrial Property Number
      2007-106303
    • Filing Date
      2007-04-13
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Patent(Industrial Property Rights)] 刺激応答計測装置および刺激奥羽計測法2007

    • Inventor(s)
      口岩 聡, 口岩 俊子
    • Industrial Property Rights Holder
      鹿児島大学
    • Industrial Property Number
      2007-106303
    • Filing Date
      2007-04-13
    • Related Report
      2006 Annual Research Report
  • [Patent(Industrial Property Rights)] 脳神経細胞への薬物の標的化剤2006

    • Inventor(s)
      口岩 聡, 口岩俊子
    • Industrial Property Rights Holder
      鹿児島大学
    • Industrial Property Number
      2006-015320
    • Filing Date
      2006-01-24
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Patent(Industrial Property Rights)] 脳神経細胞の薬物の標的化剤2006

    • Inventor(s)
      口岩 聡, 口岩 俊子
    • Industrial Property Rights Holder
      鹿児島大学
    • Industrial Property Number
      2006-015320
    • Filing Date
      2006-01-24
    • Related Report
      2006 Annual Research Report
  • [Publications] Cheng, S.-B他: "Up-regulation of methionine-enkephalin-like immunoreactivity by 2,3,7,8-tetrachlorodibenzo-p-dioxin treatment in the forebrain of the rat."Journal of Chemical Neuroanatomy. 25. 73-82 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Cheng, S.-B, 他: "Dioxin exposure down-regulates nitric oxide synthase and NADPH-diaphorase activities in the hypothalamus of Long-Evans rat."Neuroscience Letters. 345. 5-8 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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