The Study on Proteinaceous Toxins from the Cnidarians.
| Project/Area Number |
15510172
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Living organism molecular science
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| Research Institution | Tokyo University of Marine Science and Technology (2004-2005)
東京水産大学 (2003) |
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Principal Investigator |
NAGAI Hiroshi Tokyo University of Marine Science and Technology, Department of Ocean Science, Associate Professor, 海洋科学部, 助教授 (50291026)
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| Co-Investigator(Kenkyū-buntansha) |
NAMIKOSHI Michio Tokyo University of Marine Science and Technology, Department of Ocean Science, Professor, 海洋科学部, 教授 (30189196)
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| Project Period (FY) |
2003 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2005: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2004: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2003: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Chiropsalmus quadrigatus / protein toxin / cytotoxicity / hemolytic activity / Actineria villosa / fire coral / アカクラゲ / ミズクラゲ / タンパク質 / 毒素 / 致死活性 / ウンバチイソギンチャク / PsTX-60B / 刺胞動物 / AvTX-60A / MACPF |
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| Research Abstract |
From the fiscal year 2003 to 2005, we have mainly studied on the isolation and chemical characterization of proteinaceous toxins obtained from Okinawan marine venomous animals. During these studies, we have found out the box jellyfish Chiropsalmus quadrigatus (habukurage in Japanese) has several proteinaceous toxins of which mode of action differs to each other. This result suggests that the stinging syndrome by C.quadrigatus is composed of multiple symptoms. The Okinawan sea anemone Actineria villosa causes severe cases of stinging. However, the causative toxins of the stinging by this species have not yet been known. Subsequently, we isolated the proteinaceous toxin which designated as A.viliosa toxin 60A (AvTX-60A, ca.60 kDa) as the major toxin. The cDNA encoding AvTX-60A was sequenced. The deduced amino acid sequence of AvTX-60A showed the MACPF domain. The hemolytic proteinaceous toxin has been successfully isolated from the venomous fire coral, Millepora sp. This toxin had an acidic isoelectric point and the molecular weight of around 100 kDa. This is the first reported case of the isolation of a high molecular weight (100 kDa) protein toxin as its active form from cnidarians.
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Report
(4 results)
Research Products
(56 results)
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[Journal Article] Lissoclibadins 1-3, three new polysulfur alkaloids, from the ascidian Lissoclinum cf.badium.2005
Author(s)
Hongwei Liu, Takeshi Fujiwara, Teruaki Nishikawa, Yuri Mishima, Hiroshi Nagai, Takeshi Shida, Kazuo Tachibana, Hisayoshi Kobayashi, Remy E.P.Mangindaan, Michio Namikoshi.
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Journal Title
Tetrahedron 61
Pages: 8611-8615
Description
「研究成果報告書概要(和文)」より
Related Report
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[Journal Article] Lissoclibadins 1-3, three new polysulfur alkaloids, from the ascidian Lissoclinum cf.badium.2005
Author(s)
Hongwei Liu, Takeshi Fujiwara, Teruaki Nishikawa, Yuri Mishima, Hiroshi Nagai, Takeshi Shida, Kazuo Tachibana, Hisayoshi Kobayashi, Remy E.P.Mangindaan, Michio Namikoshi
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Journal Title
Tetrahedron 61
Pages: 8611-8615
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Lissoclibadins 1-3, three new polysulfur alkaloids, from the ascidian Lissoclinum cf. badium2005
Author(s)
Hongwei Liu, Takeshi Fujiwara, Teruaki NishiKawa, Yuri Mishima, Hiroshi Nagai, Takeshi Shida, Kazuo Tachibana, Hisayoshi Kobayashi, Remy E.P.Mangindaan, Michio Namikoshi.
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Journal Title
Tetrahedron 61
Pages: 8611-8615
Related Report
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[Journal Article] Isolation of Araguspongine M, a New Stereoisomer of Araguspongine/Xestospongin alkaloid, and Dopamine from the Marine Sponge Neopetrosia exigua Collected in Palau.2004
Author(s)
Hongwei Liu, Yuri Mishima, Takeshi Fujiwara, Hiroshi Nagai, Akira Kitazawa, Yuji Mine, Hisayoshi Kobayashi, Xinsheng Yao, Junko Yamada, Taiko Oda, Michio Namikoshi.
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Journal Title
Marine Drugs 2
Pages: 154-163
Description
「研究成果報告書概要(和文)」より
Related Report
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[Journal Article] Lissoclibadin 1, a novel trimeric sulfur-bridged dopamine derivative, from the tropical ascidian Lissoclinum cf.badium.2004
Author(s)
Hongwei Liu, Silvester Benny Pratasik, Teruaki Nishikawa, Takeshi Shida, Kazuo Tachibana, Takeshi Fujiwara, Hiroshi Nagai, Hisayoshi Kobayashi, Michio Namikoshi.
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Journal Title
Tetrahedron letters 45
Pages: 7015-7017
Description
「研究成果報告書概要(和文)」より
Related Report
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[Journal Article] Isolation of Araguspongine M, a New Stereoisomer of Araguspongine/Xestospongin alkaloid, and Dopamine from the Marine Sponge Neopetrosia exigua Collected in Palau.2004
Author(s)
Hongwei Liu, Yuri Mishima, Takeshi Fujiwara, Hiroshi Nagai, Akira Kitazawa, Yuji Mine, Hisayoshi Kobayashi, Xinsheng Yao, Junko Yamada, Taiko Oda, Michjo Namikoshi
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Journal Title
Marine Drugs 2
Pages: 154-163
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Lissoclibadin 1, a novel trimeric sulfur-bridged dopamine derivative, from the tropical ascidian Lissoclinum cf.badium.2004
Author(s)
Hongwei Liu, Silvester Benny Pratasik, Teruaki Nishikawa, Takeshi Shida, Kazuo Tachibana, Takeshi Fujiwara, Hiroshi Nagai, Hisayoshi Kobayashi, Michio Namikoshi
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Journal Title
Tetrahedron letters 45
Pages: 7015-7017
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Isolation of Araguspongine M, a New Stereoisomer of Araguspongine/Xestospongin alkaloid, and Dopamine from the Marine Sponge Neopetrosia exigua Collected in Palau2004
Author(s)
H.Liu, Y.Mishima, T.Fujiwara, H.Nagai, A.Kitazawa, Y.Mine, H.Kobayashi, X.Yao, J.Yamada, T.Oda, M.Namikoshi
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Journal Title
Marine Drugs 2
Pages: 154-163
Related Report
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[Journal Article] Lissoclibadin 1, a novel trimeric sulfur-bridged dopamine derivative, from the tropical ascidian Lissoclinum cf. badium2004
Author(s)
H.Liu, S.B.Pratasik, T.Nishikawa, T.Ishida, K.Tachibana, T.Fujiwara, H.Nagai, H.Kobayashi, M.Namikoshi
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Journal Title
Tetrahedron letters 45
Pages: 7015-7017
Related Report
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[Journal Article] A Trial of MASS Spectrometric Characterization on Novel Animal Toxins in Femto-molar Amount from the Subtoropical Islands.2003
Author(s)
Terumi Nakajima, Hideo Naoki, Gerardo Corzo, Dai Li, Miki Hisada, Pierre Escoubas, Naoko Yamaji, Hiroshi Nagai, Akikazu Yasuda, Marta Andrianstiferana, Joachim Haupt, Naomasa Ohshiro
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Journal Title
J.Toxicol-Toxin Rev. 22
Pages: 509-520
Description
「研究成果報告書概要(和文)」より
Related Report
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