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Two intercellular lamellar structures in stratum corneum and their functional regulation mechanism

Research Project

Project/Area Number 15540397
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biophysics/Chemical physics
Research InstitutionFukui University of Technology

Principal Investigator

HATTA Ichiro  Fukui University of Technolgy, Faculty of Engineering, Professor, 工学部, 教授 (70016070)

Co-Investigator(Kenkyū-buntansha) MORISHIMA Yotaro  Fukui University of Technology, Faculty of Engineering, Professor, 工学部, 教授 (70028249)
OHTA Noboru  Fukui University of Technolgy, Spring-8, Researcher, 協力研究員 (90353507)
Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2004: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2003: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordsskin / stratum corneum / lipid / intercellular lipid / hydrocarbon chain / X-ray diffraction / differential scanning calorimetry / 角層 / 温度依存性 / 角質層 / 脂質 / コレステロール / 経皮吸収
Research Abstract

The outermost layer of skin, stratum corneum (SC), is composed of corneocytes and intercellular lipid matrix. The matrix works as not only the main barrier but also the pathway of water, drug, etc. In mammalian SCs, for the longitudinal arrangement of the lipid molecules, long and short lamellar structures with a repeat distance of about 13 nm and about 6 nm, respectively, have been observed by small-angle X-ray diffraction. With increasing water content in SC, the diffraction peak position for the diffraction of long lamellar structure (LLS) is almost unchanged. On the other hand, the position of the diffraction of short lamellar structure (SLS) markedly shifts towards lower angle, suggesting that SLS exhibits swelling with the increase of the water content. Furthermore, we found that the widths of the diffraction peaks of both LLS and SLS become narrow simultaneously at a water content of 20-30 wt%. The present results indicate that LLS and SLS interact with each other, swelling of SLS takes place, and as a result at the water content of 20-30 wt% the both LLS and SLS are stabilized simultaneously. Besides, for the lateral arrangement of the lipid molecules, hexagonal and orthorhombic hydrocarbon chain packings have been observed by wide-angle X-ray diffraction. From the temperature dependence of the simultaneous small- and wide-angle X-ray diffraction, we revealed that the intercellular lipid matrix forms two domains which, at room temperature, are composed of the long lamellar structure with the hexagonal hydrocarbon-chain packing and the short lamellar structure with the orthorhombic hydrocarbon-chain packing.

Report

(4 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (19 results)

All 2005 2004 2003 Other

All Journal Article (16 results) Publications (3 results)

  • [Journal Article] Structural analysis of cell membrance complex of a hair fiber by micro-beam X-ray diffraction2005

    • Author(s)
      Noboru Ohta
    • Journal Title

      J. Appl. Cryst. 38

      Pages: 274-279

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Thermal Analysis of Stratum Corneum of Hairless Mouse with Attention to Phase Transitions near 35℃2005

    • Author(s)
      Ichiro Hatta
    • Journal Title

      Thermochim. Acta 431

      Pages: 94-97

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Temperature scanning X-ray diffraction at phase transitions of biological related lipid assemblies---Special attention to cholesterol-rich state---2005

    • Author(s)
      Ichiro Hatta
    • Journal Title

      J. Thermal Anal. Cal. 82

      Pages: 189-192

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Structural analysis of cell membrane complex of a hair fiber by micro-beam X-ray diffraction2005

    • Author(s)
      Noboru Ohta, Toshihiko Oka, Katsuaki Inoue, Naoto Yagi, Satoru Kato, Ichiro Hatta
    • Journal Title

      J.Appl.Cryst. 38

      Pages: 274-279

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Thermal Analysis of Stratum Corneum of Hairless Mouse with Attention to Phase Transitions near 35℃2005

    • Author(s)
      Ichiro Hatta, Kana Nakanishi, Kazuhiko Ishikiriyama
    • Journal Title

      Thermochim.Acta 431

      Pages: 94-97

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Temperature scanning X-ray diffraction at phase transitions of biological related lipid assemblies---Special attention to cholesterol-rich state---2005

    • Author(s)
      I.Hatta
    • Journal Title

      J.Thermal Anal.Cal. 82

      Pages: 189-192

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Structural analysis of cell membrane complex of a hair fiber by micro-beam X-ray diffraction2005

    • Author(s)
      Noboru Ohta
    • Journal Title

      J.Appl.Cryst. 38

      Pages: 274-279

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Thermal Analysis of Stratum Corneum of Hairless Mouse with Attention to Phase Transitions near 35℃2005

    • Author(s)
      Ichiro Hatta
    • Journal Title

      Thermochim.Acta 431

      Pages: 94-97

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Temperature scanning X-ray diffraction at phase transitions of biological related lipid assemblies---Special attention to cholesterol-rich state---2005

    • Author(s)
      Ichiro Hatta
    • Journal Title

      J.Thermal Anal.Cal. 82

      Pages: 189-192

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Molecular mechanism for regulation of water content in mammalian skin2004

    • Author(s)
      Ichiro Hatta
    • Journal Title

      Photon Factory Activity Report 2003 Part A Highlights

      Pages: 49-50

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Molecular mechanism for regulation of water content in mammalian skin2004

    • Author(s)
      I.Hatta, N.Ohta
    • Journal Title

      Photon Factory Activity Report 2003 Part A, Highlights

      Pages: 49-50

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Molecular mechanism for regulation of water content in mammalian skin2004

    • Author(s)
      I.Hatta, N.Ohta
    • Journal Title

      Photon Factory Activity Repoert 2003 Part A, No21

      Pages: 49-50

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Swelling of intercellular lipid lamellar structure with short repeat distance in hairless mouse stratum corneum as studied by X-ray diffraction2003

    • Author(s)
      Noboru Ohta
    • Journal Title

      Chem. Phys. Lipids 123

      Pages: 1-8

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] ac nanocalorimeter for measuring heat capacity of biological macromolecules in solution2003

    • Author(s)
      Haruhiko Yao
    • Journal Title

      Rev. Sci. Instrum. 74

      Pages: 4164-4168

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Swelling of intercellular lipid lamellar structure with short repea distance in hairless mouse stratum corneum as studied by X-ray diffraction2003

    • Author(s)
      N.Ohta, S.Ban, H.Tanaka, S.Nakata, I.Hatta
    • Journal Title

      Chem.Phys.Lipids 123

      Pages: 1-8

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] ac nanocalorimeter for measuring heat capacity of biologic macromolecules in solution2003

    • Author(s)
      H.Yao, K.Ema, H.Fukada, K.Takahashi, I.Hatta
    • Journal Title

      Rev.Sci.Instrum 74[9]

      Pages: 4164-4168

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Publications] N.Ohta, S.Ban, H.Tanaka, S.Nakata, I.Hatta: "Swelling of intercellular lipid lamellar structure with short repeat distance in hairless mouse stratum corneum as studied by X-ray diffraction"Chem.Phys.Lipids. 123・1. 1-8 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] H.Yao, K.Ema, H, Fukada, K.Takahashi, I.Hatta: "ac nanocalorimeter for measuring heat capacity of biological macromolecules in solution"Rev.Sci.Instrum.. 74・9. 4164-4168 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 八田一郎: "皮膚の測定・評価マニュアル集 第12章 角質細胞間脂質を測定したい,第1節 角層の細胞間脂質がつくるミクロ構造の評価 [1]X線構造解析"技術情報協会. 169-182 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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