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Conformation of Peptide Model Molecules in Alcohol-Water Mixtures and Its Pressure Dependence

Research Project

Project/Area Number 15550016
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Physical chemistry
Research InstitutionSaga University

Principal Investigator

TAKAMUKU Toshiyuki  Saga University, Faculty of Science and Engineering, Department of Chemistry and Applied Chemistry, Associate Professor, 理工学部, 助教授 (70291838)

Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2004: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2003: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsProtein / Folding / Aliphatic alcohol / Fluoroalcohol / Cluster / SANS / Dehydration / Hydrophobic interaction / ペプチドモデル分子 / ハロゲノアルコール / NMR化学シフト / 赤外分光法 / 溶存挙動 / エタノール / トリフルオロエタノール / ケミカルシフト / 外部複基準法 / コンフォメーション
Research Abstract

The present investigation has focused on alcohol-induced protein folding to clarify its mechanism at fee molecular level. Firstly, structure and size of solvent clusters predominantly formed in various alcohol-water mixtures have been investigated by using X-ray and neutron scattering techniques. It has been shown that the larger the hydrophobic group of alcohols, the more the tetrahedral-like structure of water is disrupted at low alcohol concentration, result in that alcohol molecules easily aggregate to form solvent clusters. In particular, the results from small-angel neutron scattering experiments have shown that fluoroalcohols of trifluoroethanol(TFE) and hexafluoroisopropanol(HFIP) significantly form solvent clusters in their aqueous solutions. These findings, together with the fact that the fluoroalcohols more strongly induce protein folding than aliphatic alcohols, such as ethanol(EtOH) and 2-propanol(2-PrOH), suggest the relation between cluster formation and protein folding.
Solvation behavior of 1,4-pentanediol molecule, which involves a hydrophobic hydrocarbon chain and two hydrophilic hydroxyl groups, as a model of peptide molecule in various alcohol-water mixtures has been investigated by using infrared spectroscopy and NMR. The results have suggested that addition of ethanol and 2-propanol into aqueous 1,4-pentanediol solution induces to dehydration from the hydroxyl groups of 1,4-pentanediol molecule, whereas TFE and HFIP strongly interact to the hydrocarbon chain. It has also been shown that the hydrophobic interaction between alcohol molecule and the hydrocarbon chain of 1,4-pentanediol molecule is more stronger in the sequence of HFIP>TFE>EtOH【approximately equal】2-PrOH. This sequence is in agreement with that for the effect of alcohol on protein folding. The present investigation has suggested that interaction between alcohol clusters and protein molecule significantly contributes to protein folding.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (37 results)

All 2005 2004 2003 Other

All Journal Article (29 results) Book (1 results) Publications (7 results)

  • [Journal Article] Structure of Methanol Confined in MCM-41 Investigated by Large-Angle X-ray Scattering Technique2005

    • Author(s)
      T.Takamuku ほか4名
    • Journal Title

      J.Phys.Chem.B 109

      Pages: 892-899

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] X-ray Diffraction Studies on Methanol-Water, Ethanol-Water, and 2-Propanol-Water Mixtures at Low Temperatures2005

    • Author(s)
      T.Takamuku ほか3名
    • Journal Title

      J.Mol.Liquids 119

      Pages: 133-146

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Neutron Diffraction Study on Microinhomogeneities in Ethanol-Water Mixtures2005

    • Author(s)
      T.Yamaguchi ほか2名
    • Journal Title

      J.Neutron Research 13

      Pages: 129-133

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Structure of Methanol Confined in MCM-41 Investigated by Large-Angle X-ray Scattering Technique2005

    • Author(s)
      T.Takamuku with other 4 authors
    • Journal Title

      J.Phys.Chem.B 109

      Pages: 892-899

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] X-ray Diffraction Studies on Methanol-Water, Ethanol-Water, and 2-Propanol-Water Mixtures at Low Temperatures2005

    • Author(s)
      T.Takamuku with other 3 authors
    • Journal Title

      J.Mol.Liquids 119

      Pages: 133-146

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Neutron Diffraction Study on Microinhomogeneities in Ethanol-Water Mixtures2005

    • Author(s)
      T.Yamaguchi with other 2 authors
    • Journal Title

      J.Neutron Research 13

      Pages: 129-133

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Neutron Diffraction Study on Microinhomogeneities in Ethanol-Water Mixtures2005

    • Author(s)
      T.Yamaguchi ほか2名
    • Journal Title

      J. Neutron Research 13

      Pages: 129-133

    • Related Report
      2004 Annual Research Report
  • [Journal Article] NMRで観測する生体関連分子の溶媒和2004

    • Author(s)
      高椋利幸
    • Journal Title

      ぶんせき 11号

      Pages: 675-675

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Structure of 1-Propanol-Water Mixtures Investigated by Large-Angle X-ray Scattering Technique2004

    • Author(s)
      T.Takamuku ほか3名
    • Journal Title

      J.Solution Chem. 33

      Pages: 641-660

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Liquid Structure of 1-Propanol by Molecular Dynamics Simulation and X-ray Scattering2004

    • Author(s)
      I.Akiyama ほか5名
    • Journal Title

      J.Solution Chem. 33

      Pages: 797-809

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Solvation of Bio-related Molecules Observed with NMR (in Japanese)2004

    • Author(s)
      T.Takamuku
    • Journal Title

      Bunseki (11)

      Pages: 675-675

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Structure of 1-Propanol-Water Mixtures Investigated by Large-Angle X-ray Scattering Technique2004

    • Author(s)
      T.Takamuku with other 3 authors
    • Journal Title

      J.Solution Chem. 33

      Pages: 641-660

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Liquid Structure of 1-Propanol by Molecular Dynamics Simulation and X-ray Scattering2004

    • Author(s)
      I.Akiyama with other 5 authors
    • Journal Title

      J.Solution Chem. 33

      Pages: 797-809

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Structure of 1-Propanol-Water Mixtures Investigated by Large-Angle X-ray Scattering Technique2004

    • Author(s)
      T.Takamuku ほか3名
    • Journal Title

      J. Solution Chem. 33

      Pages: 641-660

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Liquid Structure of 1-Propanol by Molecular Dynamics Simulation and X-ray Scattering2004

    • Author(s)
      I.Akiyama ほか5名
    • Journal Title

      J. Solution Chem. 33

      Pages: 797-809

    • Related Report
      2004 Annual Research Report
  • [Journal Article] NMRで観測する生体関連分子の溶媒和2004

    • Author(s)
      高椋利幸
    • Journal Title

      ぶんぜき 11号

      Pages: 675-675

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Structure of Aqueous Mixtures of N, N-Dimethylacetamide Studied by Infrared Spectroscopy X-ray Diffraction, and Mass Spectrometry2003

    • Author(s)
      T.Takamuku ほか4名
    • Journal Title

      J.Phys.Chem.B 107

      Pages: 6070-6078

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Structure and Dynamics of Hexafluoroisopropanol-Water Mixtures by X-ray Diffraction Small-Angle Neutron Scattering, NMR Spectroscopy, and Mass Spectrometry2003

    • Author(s)
      K.Yoshida ほか6名
    • Journal Title

      J.Chem.Phys. 119

      Pages: 6132-6142

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] X線回折法によるMCM-41細孔中に閉じ込められたメタノールの構造解析2003

    • Author(s)
      丸山浩和 ほか4名
    • Journal Title

      X線分析の進歩 34

      Pages: 227-239

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 混合溶媒の液体構造と相分離を用いる分析化学2003

    • Author(s)
      高椋利幸 ほか1名
    • Journal Title

      ぶんせき 5号

      Pages: 234-240

    • NAID

      80015915182

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Alkali Chlorides-Induced Phase Separation of Acetonitrile-Water Mixtures Studied by Small-Angle Neutron Scattering Technique2003

    • Author(s)
      T.Takamuku ほか4名
    • Journal Title

      Activity Report on Neutron Scattering Research 10

      Pages: 182-183

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Small-Angle Neutron Scattering Study on Cluster Formation in TFE-Water Mixtures2003

    • Author(s)
      T.Takamuku ほか4名
    • Journal Title

      Activity Report on Neutron Scattering Research 10

      Pages: 184-185

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Structure of Aqueous Mixtures of N,N-Dimethylacetamide Studied by Infrared Spectroscopy X-ray Diffraction, and Mass Spectrometry2003

    • Author(s)
      T.Takamuku with other 4 authors
    • Journal Title

      J.Phys.Chem., B 107

      Pages: 6070-6078

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Structure and Dynamics of Hexafluoroisopropanol-Water Mixtures by X-ray Diffraction Small-Angle Neutron Scattering, NMR Spectroscopy, and Mass Spectrometry2003

    • Author(s)
      K.Yoshida with other 6 authors
    • Journal Title

      J.Chem.Phys. 119

      Pages: 6132-6142

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A Study on Structure of Methanol Confined in Mesoporous MCM-41 by X-ray Diffraction (in Japanese)2003

    • Author(s)
      H.Maruyama with other 4 authors
    • Journal Title

      Adv.X-ray Anal.Jpn. 34

      Pages: 227-239

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Structure of Mixed Solvents and Their Phase Separation Applied to Analytical Chemistry (in Japanese)2003

    • Author(s)
      T.Takamuku with other 1 author
    • Journal Title

      Bunseki (5)

      Pages: 234-240

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Alkali Chlorides-Induced Phase Separation of Acetonitrile-Water Mixtures Studied by Small-Angle Neutron Scattering Technique2003

    • Author(s)
      T.Takamuku with other 4 authors
    • Journal Title

      Activity Report on Neutron Scattering Research 10

      Pages: 182-183

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Small-Angle Neutron Scattering Study on Cluster Formation in TFE-Water Mixtures2003

    • Author(s)
      T.Takamuku with other 4 authors
    • Journal Title

      Activity Report on Neutron Scattering Research 10

      Pages: 184-185

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] X-ray Diffraction Studies on Methanol-Water, Ethanol-Water, and 2-Propanol-Water Mixtures at Low Temperatures

    • Author(s)
      T.Takamuku ほか3名
    • Journal Title

      J.Mol.Liquids (in press)

    • Related Report
      2004 Annual Research Report
  • [Book] 水ハンドブック(水ハンドブック編集委員会編)2003

    • Author(s)
      高椋利幸(分担著)
    • Publisher
      丸善(東京)
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] T.Takamuku: "Structure of Aqueous Mixtures of N,N-Dimethylacetamide Studied by Infrared Spectroscopy X-ray Diffraction, and Mass Spectrometry"J.Phys.Chem.B. 107. 6070-6078 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] K.Yoshida: "Structure and Dynamics of Hexafluoroisopropanol-Water Mixtures by X-ray Diffraction Small-Angle Neutron Scattering, NMR Spectroscopy, and Mass Spectrometry"J.chem.Phys.. 119. 6132-6142 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] T.Takamuku: "Structure of 1-Propanol-Water Mixtures Investigated by Large-Angle X-ray Scattering Technique"J.Solution Chem.. (in press).

    • Related Report
      2003 Annual Research Report
  • [Publications] 高椋利幸: "混合溶媒の液体構造と相分離を用いる分析化学"ぶんせき. 5号. 234-240 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] T.Takamuku: "Alkali Chlorides-Induced Phase Separation of Acetonitrile-Water Mixtures Studied by Small-Angle Neutron Scattering Technique"Activity Report on Neutron Scattering Research. 10. 182-183 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] T.Takamuku: "Small-Angle Neutron Scattering Study on Cluster Formation in TFE-Water Mixtures"Activity Report on Neutron Scattering Research. 10. 184-185 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 高椋利幸(共著): "水ハンドブック(水ハンドブック編集委員会編)"丸善(東京). 4 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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