| Project/Area Number |
15550073
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Analytical chemistry
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
NAKANO Koji Kyushu University, Faculty of Engineering, Associate Professor, 工学研究院, 助教授 (10180324)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2004: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2003: ¥2,700,000 (Direct Cost: ¥2,700,000)
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| Keywords | DNA conjugate / gene sensor / DNA microarray / SECM / high-throughput assay |
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| Research Abstract |
This research project has involved 1)enzymic synthesis of poly(hydroquinone), PQ, 2)chemically modified electrode preparations and gene sensor applications, and finally, 3)scanning electrochemical microscopic(SECM) imaging of DNA microarray. First, we synthesized the redox polymer by enzymic oxidation of albutin to form poly(hydroquinone glycoside) derivative, which was subsequently converted to PQ by chemical removal of the sugar-protecting group. The polymer was then applied to DNA-conjugate modified electrode having a single-stranded oligodeoxyribonucleotide (ODN)/PQ surface structure. Electrochemical studies showed that the electrode was responsive to the hybridization of the surface-anchored ODN by changing its redox activity. By detailed studied on the electrochemistry and the bioaffinity reaction, the DNA-conjugate modified electrode was concluded to be a candidate of electrochemical sensor capable of detection of specific genes. As the start point of high-throughput analytical device development, we made an attempt of two-dimensional imaging of the specific electrochemical activity of the DNA conjugate electrode using a SECM. We have finally succeeded 1)2D imaging of the DNA microdots (20 micron) and 2)detection of DNA hybridization through 2D imaging by monitoring the redox activity of the microdot.
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