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Super catalytic antbodies against Infuenza virus

Research Project

Project/Area Number 15560678
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biofunction/Bioprocess
Research InstitutionHiroshima Prefectural University

Principal Investigator

HIFUMI Emi  Hiroshima Prefectural University, School of Bioresources, Assislant Professor, 生物資源学部, 助手 (90254606)

Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2004: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2003: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsInfluenza / Antibody / Protease / Hemagglutinin(HA) / Hemagglutinin(HA) / インフルエンザウィルス / heamagglutinin / 高度保存領域 / 細胞融合
Research Abstract

Super catalytic antibody possesses highly characteristic features so as to not only specially bind to the antigen but also enzymatically decompose the antigen. It has been desired to prepare the super catalytic antibody targeting the conservative region of crucial proteins of microorganism such as bacteria and viruses. In this study, a surface protein of influenza virus was targeted for this purpose. In the following, some interesting results obtained so far are briefly described.
1, In the surface protein, hemagglutinin, of influenza virus type HINT and H2N2 there two conserved regions (TGLRN and GITNKVNSVIEK). The two peptides were synthesized as a one linear or cyclic peptide as an immunogen. The peptides were conjugated with h-IgG for immunization. And two kinds of monoclonal antibodies were obtained by using cell fusion technique.
2, The mRNAs were extracted from the hybridomas secreting monoclonal antibodies for investigating the cDNA sequence of the monoclonal antibodies. The germline genes of the light chains of the two monoclonal antibodies belonged to 8-12 and the heavy chains to 3558.46.
3, By using the deduced amino acid sequence from the cDNA, the structural analysis of the monoclonal antibodies were conducted by a work station. I found the discrete amino acid sequence possessing the catalytic activity in the structures.
4, Based on the above results, the light and heavy chains were purified and isolated from the monoclonal antibodies. The peptidase activity tests of the light and heavy chains are being performed at the present time. The peptide substrate was cleaved by those antibody subunits.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (13 results)

All 2005 2004 2003 Other

All Journal Article (11 results) Patent(Industrial Property Rights) (1 results) Publications (1 results)

  • [Journal Article] Investigation of active form of catalytic antibody light chain 41S-2-L2005

    • Author(s)
      Y.Mitsuda, K.Tsuruhata, E.Hifumi, M.Takagi, T.Uda
    • Journal Title

      Immunol.Lett. 96

      Pages: 63-71

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Investigation of active form of catalytic antibody light chain 41S-2-L2005

    • Author(s)
      Y.Mitsuda, K.Tsuruhata, E.Hifumi, M.Takagi, T.Uda
    • Journal Title

      Immunol.Lett. 96(1)

      Pages: 63-71

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Catalytic antibody light chain capable of cleaving a chemokine receptor CCR-5 peptide with a high reaction rate constant.2004

    • Author(s)
      Y.Mitsuda, E.Hifumi, K.Tsuruhata, H.Fujinami, N.Yamamoto, T.Uda
    • Journal Title

      Biotechnol.Bioeng. 86

      Pages: 217-225

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Super catalytic antibody and "Antigenase"2004

    • Author(s)
      T.Uda, E.Hifumi
    • Journal Title

      J.Biosci, Bioeng. 97

      Pages: 143-152

    • NAID

      110002665276

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 酵素と抗体は兄弟?2004

    • Author(s)
      宇田泰三, 一二三恵美
    • Journal Title

      高分子 53

      Pages: 859-863

    • NAID

      10013716774

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Catalytic antibody light chain capable of cleaving a chemokine receptor CCR-5 peptide with a high reaction rate constant.2004

    • Author(s)
      Y.Mitsuda, E.Hifumi, K.Tsuruhata, H.Fujinami, N.Yamamoto, T.Uda
    • Journal Title

      Biotechnol.Bioeng. 86(2)

      Pages: 217-225

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Endopeptidase character of monoclonal antibody i41-7 subunits2003

    • Author(s)
      K.Hatiuchi, E.Hifumi, Y.Mitsuda, T.Uda.
    • Journal Title

      Immunol.Lett. 86

      Pages: 249-257

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Catalytic features of monoclonal antibody i41SL1-2 subunits2003

    • Author(s)
      E.Hifumi, H.Kondo, Y.Mutsuda, T.Uda
    • Journal Title

      Biotech.Bioeng. 84

      Pages: 485-493

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Endopeptidase character of monoclonal antibody i41-7 subunits2003

    • Author(s)
      K.Hatiuchi, E.Hifumi, Y.Mitsuda, T.Uda
    • Journal Title

      Immunol.Lett. 86

      Pages: 249-257

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Catalytic features of monoclonal antibody i41SL1-2 subunits.2003

    • Author(s)
      E.Hifumi, H.Kondo, Y.Mutsuda, T.Uda
    • Journal Title

      Biotechnol.Bioeng. 84

      Pages: 485-493

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Enzyme and anibody are brothers?

    • Author(s)
      T.Uda, E.HIFUMI
    • Journal Title

      Kobunnsi 53

      Pages: 859-863

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Patent(Industrial Property Rights)] インフルエンザウィルスのヘマグルチニンに対する抗体酵素2005

    • Inventor(s)
      宇田 泰三, 一二三 恵美, 高尾信一
    • Industrial Property Rights Holder
      広島県
    • Filing Date
      2005-06-14
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] 城平直樹, 一二三恵美, 宇田泰三: "Influenza A型Hemagglutininの保存領域に対するモノクローナル抗体の作製と抗体軽鎖の抗原分解活性"日本化学会第84春季年会講演予稿集. 2巻. 1156 (2004)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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