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Studies on the Arabidopsis LINE, ATLNs : Their expression and the function of ORF proteins encoded.

Research Project

Project/Area Number 15570003
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Genetics/Genome dynamics
Research InstitutionThe University of Tokyo

Principal Investigator

OHTSUBO Hisako  The University of Tokyo, The Institute of Molecular and Cellular Biosciences, Associate Professor, 分子細胞生物学研究所, 講師 (20158801)

Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2004: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2003: ¥2,100,000 (Direct Cost: ¥2,100,000)
KeywordsLINE / Internal promoters / ORF1 protein / Leucine zipper / Nuclear localization signal / Zinc finger / Two hybrid system / Arabidopsis thaliana / ORF1 / 核局在 / タンパク-タンパク相互作用
Research Abstract

The Arabidopsis thaliana genome has LINEs, called ATLNs, whose expression is negatively regulated by DNA methylation. Analysis of deletion in the 5' UTR revealed that the 5' UTR has two promoters, designated here as P1 and P2. Analysis of transcripts by 5' RACE showed that their 5' ends were located at sites immediately upstream of the P1 region or at sites downstream of the P2 region. This observation and the fact that the P1 region contains no TATA sequence indicate that P1 is an internal promoter that initiates transcription from sites upstream of the promoter.
ATLN members have two consecutive open reading frames (orf1 and orf2) that are essential for retrotransposition. The C-terminal region of the ORF1 protein has nuclear localization signal (NLS) -like sequences, KRKR and KKGRK. The GFP-ORF1 fusion protein, which was expressed in tobacco BY-2 and onion skin cells, was localized to the nuclei of their cells, but the GFP-ORF1-fusion protein with mutations in NLS-like sequences was not. Because the N-terminal region of the ORF1 protein has a retroviral zinc-finger motif, we also examined protein-protein interactions by using two hybrid system in yeast as well as in tobacco BY2 cells. We found that ORF1 can interact with each other, whereas ORF1 with substitution mutations in the zinc-finger motif as well as in the leucine-zipper motif cannot. These results show that ORF1 consists of two kinds of functional domains involved in nuclear localization and protein-protein interaction.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (15 results)

All 2005 2004 2003 Other

All Journal Article (13 results) Publications (2 results)

  • [Journal Article] Phylogenetic analysis of Oryza rufipogon strains and their relations to Oryza sativa strains by insertion polymorphism of rice SINEs.2005

    • Author(s)
      Xu, J.-H., Cheng C., Tsuchimoto, S., Ohtsubo, H., Ohtsubo, E.
    • Journal Title

      Genes and Genetic Systems 80(In press)

    • NAID

      10024394358

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] レトロエレメントからみたイネゲノム2005

    • Author(s)
      大坪 久子
    • Journal Title

      蛋白質・核酸・酵素 50

      Pages: 215-227

    • NAID

      40006635192

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] 「研究成果報告書概要(欧文)」より2005

    • Author(s)
      Xu, J.-H., Cheng C., Tsuchimoto, S., Ohtsubo, H., Ohtsubo, E.
    • Journal Title

      Genes and Genetic Systems (in press)

    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 「研究成果報告書概要(欧文)」より2005

    • Author(s)
      Ohtsubo, H.
    • Journal Title

      Protein, Nucleic Acid and Enzyme 50

      Pages: 215-227

    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Australian wild rice species Oryza meridionalis consists of annual and perennial ecotype strains.2005

    • Author(s)
      Xu, J.-H., Kurata, N., Akimoto, M., Ohtsubo, H., Ohtsubo, E.
    • Journal Title

      Genes and Genetic Systems (印刷中)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Rice retroposon p-SINE1 and origin of the cultivated rice.2004

    • Author(s)
      Ohtsubo, H., Cheng, C., Tsuchimoto, S., Ohtsubo E.
    • Journal Title

      Breeding Sci. 58

      Pages: 1-11

    • NAID

      110001803259

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Intermediate molecules generated by transposase in the pathways of transposition of bacterial insertion element IS32004

    • Author(s)
      Ohtsubo, E.Minematsu, H.Tsuchida, K., Ohtsubo, H., Sekine, Y.
    • Journal Title

      Adv.Biophys. 38

      Pages: 125-139

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 「研究成果報告書概要(欧文)」より2004

    • Author(s)
      Ohtsubo, H., Cheng, C., Tsuchimoto, S.Osawa, I., Ohtsubo, E.
    • Journal Title

      Breeding Sci. 58

      Pages: 1-11

    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 「研究成果報告書概要(欧文)」より2004

    • Author(s)
      Ohtsubo, E., Minematsu, H., Tsuchida, K., Ohtsubo, H., Sekine, Y.
    • Journal Title

      Adv.Biophys. 38

      Pages: 125-139

    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Rice retroposon p-SINE1 and origin of the cultivated rice.2004

    • Author(s)
      Ohtsubo, H., Cheng, C., Tsuchimoto, S., Ohtsubo E.
    • Journal Title

      Breed.Sci. 58

      Pages: 1-11

    • NAID

      110001803259

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Intermediate molecules generated by transposase in the pathways of transposition of bacterial insertion element IS32004

    • Author(s)
      Ohtsubo, E., Minematsu, H., Tsuchida, K., Ohtsubo, H., Sekine, Y.
    • Journal Title

      Adv.Biophys. 38

      Pages: 125-139

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Polyphyletic origin of cultivated rice : based on the interspersion pattern of SINEs.2003

    • Author(s)
      Cheng, C., Motohashi, R., Tsuchimoto, S, Fukuta, Y., Ohtsubo H., Ohtsubo E.
    • Journal Title

      Mol.Biol.Evol 20

      Pages: 67-75

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] 「研究成果報告書概要(欧文)」より2003

    • Author(s)
      Cheng, C., Motohashi, R., Tsuchimoto, S., Fukuta, Y., Ohtsubo H., Ohtsubo E.
    • Journal Title

      Mol.Biol.Evol. 20

      Pages: 67-75

    • Related Report
      2004 Final Research Report Summary
  • [Publications] Cheng, C.: "Polyphyletic origin of cultivated rice."Mol.Biol.Evol.. 20. 67-75 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ohtsubo, H.: "Rice retrotransposon, p-SINE1 and origin of the cultivated rice"Breeding Sci.. (in press).

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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