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A study on novel regulation mechanism to repress precocious meiotic entry in Drosophila male

Research Project

Project/Area Number 15570006
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Genetics/Genome dynamics
Research InstitutionKyoto Institute of Technology

Principal Investigator

INOUE Yoshihiro  Kyoto Institute of Technology, Drosophila Genetic Res.Center, Lecturer, ショウジョウバエ遺伝資源センター, 講師 (90201938)

Co-Investigator(Kenkyū-buntansha) YAMAGUCHI Masamitsu  Kyoto Institute of Technology, Dept.of Applied Biology, Professor, 繊維学部, 教授 (00182460)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2004: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2003: ¥2,300,000 (Direct Cost: ¥2,300,000)
KeywordsDrosophila / male / meiosis / Mitosis / Cyclin / Polo / G2 / M期進行 / LARP / Polo / 染色体凝縮 / Aurora B / LA様タンパク
Research Abstract

The G2 phase just prior to first meiotic division in male is called a growth phase that is an essential period to ensure gene expression required for meiotic divisions and later spermiogenesis in Drosophila. A timing to initiate the first meiotic division is therefore strictly regulated by activity of CDK1. A hypomorphic mutation at the mitotic gene, meteor (mtr) in Drosophila leads to a production of smaller cells in premeiotic cysts consisting of primary spermatocytes. We found that a chromosome condensation has precociously proceeded in the "wee" cells. Another distinctive features in M phase such as nuclear membrane-break-down and formation of spindle-like structure have also already occurred in these "wee" cells within premeiotic cysts. The precocious entry of meiotic divisions did not allow these cells to grow sufficiently. These cytological observations suggest that the mtr gene is involved in a negative regulation to repress initiation of first meiotic division until a completi … More on of the growth phase in Drosophila males. Surprisingly, these precocious meiotic entry in the mtr males occurred before a sufficient expression of both Cyclin A and Cyclin B. Immunostaining experiments to detect active form of CDK1 indicate that the active MPF has not yet accumulated in cytoplasm of the "wee" cells with precociously condensed chromatin in the mtr males. Consistently, this precocious meiotic entry in mtr was observed even in mutant background of twine gene encoding a Cdc25 orthologue exclusively active before meiotic division. In addition to the precocious meiotic entry, a failure of centrosome separation, spindle abnormality and non-disjunction was also observed during meiotic divisions in the mtr males. The mtr gene corresponds to dlarp that encode a Drosophila ortholog of the LA-related protein sharing a homologous domain with human Lupas Antigen. Our immunostaining experiments localized the Meteor protein to a peripheral region of nucleus at premeiotic stages and to the centrosomes at M phase. We speculate that the Meteor has essential roles in both meiotic entry and meiotic divisions in Drosophila males. Less

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (35 results)

All 2005 2004 2003 Other

All Journal Article (26 results) Book (2 results) Publications (7 results)

  • [Journal Article] Drosophila damaged DNA binding protein 1 (D-DDB1) is an essential factor for development.2005

    • Author(s)
      Takata, K., Yoshida, H., Yamaguchi, M., et al.
    • Journal Title

      Genetics in press

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Mutation in orbit/mast, reveal that the central spindle is comprised of two microtubule populations ; those that initiate cleavage and those that propagate furrow ingression.2004

    • Author(s)
      Inoue Y.H., Savoian, M., Suzuki, T., et al.
    • Journal Title

      J.Cell Biol. 166

      Pages: 49-60

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Genetic link between p53 and genes required for formation of the zonula adherens junction.2004

    • Author(s)
      Yamaguchi M, Hirose F, Inoue Y.H et al.
    • Journal Title

      Cancer Sci. 95

      Pages: 436-441

    • NAID

      10013723115

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] DREF is required for EGFR signaling during Drosophila wing vein development.2004

    • Author(s)
      Yoshida, H., Kwon, E., Hirose, F., et al.
    • Journal Title

      Genes to Cells 9

      Pages: 935-944

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Genetic link between p53 and genes required for formation of the zonula adherens junction.2004

    • Author(s)
      Yamaguchi M, Hirose F, Inoue Y.H, Ohno K, Yoshida H, Hayashi Y, Deak P, Matsukage A.
    • Journal Title

      Cancer Sci. 95

      Pages: 436-441

    • NAID

      10013723115

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Redox regulation of DNA binding activity of DREF in Drosophila.2004

    • Author(s)
      Choi, T.-Y., Park, S.Y., Kang, H.-S., Cheong, J.-H., Kim, H.-D., Lee, B.L., Hirose, F., Yamaguchi, M., Yoo, M.-A.
    • Journal Title

      Biochem.J. 378

      Pages: 833-838

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Transcriptional regulation of the Drosophila caudal homeobox gene by the DRE/DREF system.2004

    • Author(s)
      Choi, Y.-J., Choi, T.-Y., Yamaguchi, M., Matsukage, A., Kim.Y-S., Yoo, M.-A.
    • Journal Title

      Nucleic Acids Res. 32

      Pages: 3734-3742

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] DREF is required for EGFR signaling during Drosophila wing vein development.2004

    • Author(s)
      Yoshida, H., Kwon, E., Hirose, F., Otsuki, K., Yamada, M., Yamaguchi, M.
    • Journal Title

      Genes to Cells 9

      Pages: 935-944

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Armadillo/Pangolin regulates PCNA and DREF promoter activities.2004

    • Author(s)
      Kwon E., Hayashi, Y.Otsuki, K., Hirose, F., Nishida, Y., Yoo, M.-A., Yamaguchi, M.
    • Journal Title

      Biochem.Biophys.Acta. 1679

      Pages: 256-262

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Damaged DNA binding protein 1 in Drosophila defense reactions.2004

    • Author(s)
      Takata, K., Shimanouchi, K., Yamaguchi, M., Murakami, S., Ishikawa, G, Takeuchi, R., Kanai, Y., Ruike, T., Nakamura, R., Abe, Y., Sakaguchi, K.
    • Journal Title

      Biochem.Biophys.Res.Commun. 323

      Pages: 1024-1031

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Characterization of dRFX2, a novel RFX family protein in Drosophila.2004

    • Author(s)
      Otsuki, K., Hayashi, Y., Kato, M., Yoshida, H., Yamaguchi, M.
    • Journal Title

      Nucleic Acids Res. 32

      Pages: 5636-5648

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Mutation in orbit/mast, reveal that the central spindle is comprised of two microtubule populations ; those that initiate cleavage and those that propagate furrow ingression.2004

    • Author(s)
      Inoue Y.H., Savoian, M., Suzuki, T., Yamamoto, M., Glover, D.M.
    • Journal Title

      J.Cell Biol. 166

      Pages: 49-60

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Genetic Link between p53 and genes required for formation of the zonula adeherens junction.2004

    • Author(s)
      Yamaguchi, M., Hirose, F., Inoue Y.H., Ohno, K., Yoshida, H., Hayashi, Y., Deak, P., Matsukage, A
    • Journal Title

      Cancer Sci. 95

      Pages: 436-441

    • Related Report
      2004 Annual Research Report
  • [Journal Article] DREF is required for EGFR signaling during Drosophila wing vein development.2004

    • Author(s)
      Yoshida, H., Kwon, E., Hirose, F., Yamada, M., Yamaguchi, M.
    • Journal Title

      Genes to Cells 9

      Pages: 935-944

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Characterization of dRFX2, a novel RFX family protein in Drosophila.2004

    • Author(s)
      Otsuki, K., Hayashi, Y., Yoshida, H., Yamaguchi, M
    • Journal Title

      Nucleic Acids Res. 32

      Pages: 5636-5648

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Transcriptional regulation of the Drosophila caudal homeo box gene by the DRE/DREF system.2004

    • Author(s)
      Choi, Y.-J., Choi, T.-Y., Yamaguchi, M., Matsukage, A., Kim.Y.-S., Yoo, M.-A.
    • Journal Title

      Nucleic Acids Res. 32

      Pages: 3734-3742

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Drosophila damaged DNA binding protein 1 (D-DDB1) is an essential factctor for development.2004

    • Author(s)
      Takata, K., Yoshida, H., Yamaguchi, M., Sakaguchi, K
    • Journal Title

      Genetics 168

      Pages: 855-565

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Spatio-temporal expression of Drosophila mitochondrial transcription factor A during development.2003

    • Author(s)
      Takata, K, Inoue, Y.H., Hirose, F., et al.
    • Journal Title

      Cell Biology International 27

      Pages: 361-374

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Orbit/Mast, the CLASP orthologue of Drosophila, is required for asymmetric stem cell and cystocyte divisions and development of the polarised microtubule network that interonnects cocyte and nurse cells oogenesis,2003

    • Author(s)
      Mathe, E., Inoue, Y.H., Glover, D.M
    • Journal Title

      Development 130

      Pages: 901-915

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Transcription control of a gene for Drosophila transcription factor, DREF by DRE and cis-elements conserved between Drosophila melanogaster and virilis.2003

    • Author(s)
      Kwon, E., Seto, H., Hirose, F., et al.
    • Journal Title

      Gene 309

      Pages: 101-116

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Spatio-temporal expression of Drosophila mitochondrial transcription factor A during development.2003

    • Author(s)
      Takata, K, Inoue, Y.H., Hirose, F., Murakami, S., Shimanouchi, S., Sakimoto, I., Sakaguchi, K.
    • Journal Title

      Cell Biology International 27

      Pages: 361-374

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Orbit/Mast, the CLASP orthologue of Drosophila, is required for asymmetric stem cell and cystocyte divisions anddevelopment of the polarized microtubule network that interconnects oocyte and nurse cells during oogenesis.2003

    • Author(s)
      Mathe, E., Inoue, Y.H., Palframan, W., Brown, G., Glover, D.M.
    • Journal Title

      Development 130

      Pages: 901-915

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Transcription control of a gene for Drosophila transcription factor, DREF by DRE and cis-elements conserved between Drosophila melanogaster and virilis.2003

    • Author(s)
      Kwon, E., Seto, H., Hirose, F., Ohshima, N., Takahashi, Y., Nishida, Y., Yamaguchi, M.
    • Journal Title

      Gene 309

      Pages: 101-116

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Prevention of polyglutamine oligomerization and neurodegeneration by the peptide inhibitor QBP1 in Drosophila.2003

    • Author(s)
      Nagai, Y., Fujikake, N., Ohno, K., Higashiyama, H., Popiel, H.A., Rahadian, J., Yamaguchi, M., Strittmatter, W.J., Burke, J.R., Toda, T.
    • Journal Title

      Hum.Mol.Genet. 12

      Pages: 1253-1260

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Tandem duplication of alkaline phosphatase genes and polymorphism in the intergenic sequence in Bombyx mori.2003

    • Author(s)
      Itoh, M., Inoue, T., Kanamori, Y., Nishida, S., Yamaguchi, M.
    • Journal Title

      Mol.Gen.Genomics 270

      Pages: 114-120

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Drosophila damaged DNA binding protein 1(D-DDB1) is an essential factor for development.

    • Author(s)
      Takata, K., Yoshida, H., Yamaguchi, M., Shimanouchi, K., Murakami, S., Sakaguchi, K.
    • Journal Title

      Genetics (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] 発生における細胞増殖制御2004

    • Author(s)
      山口政光, 加藤容子
    • Related Report
      2004 Annual Research Report
  • [Book] 遺伝子の転写:ショウジョウバエの形質転換(名取俊二編)2003

    • Author(s)
      井上喜博, 西田育巧
    • Publisher
      広川書店
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Mathe, E., Inoue, Y.H.: "Orbit/Mast, the CLASP orthologue of Drosophila, is required for asymmetric stem cell and cystocyte divisions and development of the polarised microtubule network that interconnects oocyte and nurse cells during oogenesis"Development. 130. 901-915 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kwon, E., Seto, H., Hirose, F., Ohshima, N., Takahashi, Y., et al.: "Transcription control of a gene for Drosophila transcription factor, DREF by DRE and cis-elements conserved between Drosophila melanogaster and virilis"Gene. 309. 101-116 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Takata, K, Inoue, Y.H., Hirose, F., Murakami, S., Shimanouchi, S., Sakimoto, I., et al.: "Spatio-temporal expression of Drosophila mitochondrial transcription factor A during development"Cell Biology International. 27. 361-374 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Nagai, Y., Fujikake, N., Ohno, K., Higashiyama, H., Popiel, H.A., et al.: "Prevention of polyglutamine oligomerization and neurodegeneration by the peptide inhibitor QBP1 in Drosophila"Hum.Mol.Genet.. 12. 1253-1260 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Takata, K., Yoshida, H., Yamaguchi, M., Shimanouchi, K., et al.: "Drosophila damaged DNA binding protein 1 (D-DDB1) is an essential factor for development"Genetics. (In press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yamaguchi, M., Hirose, F., Inoue Y.H., Ohno, K., Yoshida, H., et al.: "Genetic Link between p53 and Genes Required for Formation of the Zonula Adeherens Junction"Cancer Sci.. (In press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 山口政光, 加藤容子: "増殖関連遺伝子の制御機構.発生における細胞増殖制御(竹内隆・岸本健雄編)"シュプリンガーフェアラーク東京. 198 (2004)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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