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Molecular mechanisms regulating ethylene biosynthesis in plant

Research Project

Project/Area Number 15570029
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物生理・分子
Research InstitutionChiba University

Principal Investigator

SATO Takahide  Chiba Univ., Graduate School of Science and Technology, Professor, 大学院・自然科学研究科, 教授 (60125929)

Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2005: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2004: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2003: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsEthylene / ACC synthase / AP2 / ERF family / Cucumis melo (melon) / 転写因子 / 転写調節因子
Research Abstract

Ethylene is a gaseous plant hormone that controls many aspects of plant growth and development. 1-aminocyclopropane-1-carboxylate (ACC) synthase and ACC oxidase are key enzymes in ethylene biosynthesis pathway in higher plants. A melon 1-aminocyclopropane-1-carboxylate synthase (CMe-ACS2) is a key enzyme in the ethylene biosynthesis pathway in melon, but mechanisms regulating CMe-ACS2 expression remain to be clarified. We characterized three melon AP2/ERF transcription factors (CMe-DREB1, CMe-ERF1 and CMe-ERF2), which were isolated by a yeast one-hybrid screening method using a GCCGAC sequence (DRE/CRT cis-acting element) in the CMe-ACS2 promoter. We demonstrated that CMe-DREB1 strongly bound to the GCCGAC sequence, but CMe-ERF1 and CMe-ERF2 did not. Transient expression analysis showed that only CMe-DREB1 could activate the transcription of the downstream reporter gene through the GCCGAC sequence in melon leaf cells. The expression levels of CMe-ACS2 and CMe-DREB1 in melon leaves were increased by methyl jasmonate (MeJA) treatment. These results suggest that CMe-DREB1 functions as a transcriptional activator of CMe-ACS2 in the presence of jasmonate.

Report

(4 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (9 results)

All 2006 2005 2003 Other

All Journal Article (8 results) Publications (1 results)

  • [Journal Article] lsolation and characterization of three DREB/ERF-type transcription factors from melon(Cucumis melo)2006

    • Author(s)
      S.Mizuno, Y.Hirasawa, M.Sonoda, H.Nakagawa, T.Sato
    • Journal Title

      Plant Science 170

      Pages: 1156-1163

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Isolation and characterization of three DREB/ERF-type transcription factors from melon (Cucumis melo)2006

    • Author(s)
      S.Mizuno, Y.Hirasawa, M.Sonoda, H.Nakagawa, T.Sato
    • Journal Title

      Plant Science 170

      Pages: 1156-1163

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Isolation and characterization of three DREB/ERF-type transcription factors from melon (Cucumis melo)2006

    • Author(s)
      S.Mizuno, Y.Hirasawa, M.Sonoda, H.Nakagawa, T.Sato
    • Journal Title

      Plant Science (印刷中)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Effect of p-CPA-parthenocarpic setting on the delayed ripening of netted-melon fruits2005

    • Author(s)
      J.Pariasca, T.Kato, M.Oka, T.Ohtani, Y.Yaegashi, T.Hirabayashi, H.Nakagawa, T.Sato
    • Journal Title

      Plant Growth Regulation 45

      Pages: 29-36

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Effect of p-CPA-parthenocarpic setting on the delayed ripening of netted-melon fruits,2005

    • Author(s)
      J.Pariasca, T.Kato, M.Oka, T.Ohtani, Y.Yaegashi, T.Hirabayashi, H.Nakagawa, T.Sato
    • Journal Title

      Plant Growth Regulation 45

      Pages: 29-36

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] エチレン生合成の調節機構2003

    • Author(s)
      佐藤隆英, 水野真二
    • Journal Title

      植物の生長調節 38(2)

      Pages: 187-202

    • NAID

      10011939939

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Regulation mechanisms of ethylene biosynthesis in higher plants (in Japanese)2003

    • Author(s)
      T.Sato, S.Mizuno
    • Journal Title

      Regulation of Plant Growth & Development 38

      Pages: 187-202

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Effect of p-CPA-parthenocarpic setting on the delayed ripening of netted-melon fruits

    • Author(s)
      J.Pariasca, T Kato, M Oka, T Ohtani et al.
    • Journal Title

      Plant Growth Regulation. (印刷中)

    • Related Report
      2004 Annual Research Report
  • [Publications] 佐藤隆英, 水野真二: "エチレン生合成の調節機構"植物の生長調節. 38・2. 187-202 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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