A study on expression of the gene encoding photolyase for cyclobutane pyrimidine dimer and regulation mechanism of photolyase activity
| Project/Area Number |
15570030
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物生理・分子
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| Research Institution | Teikyo University of Science & Technology (2004-2005)
The University of Tokyo (2003) |
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Principal Investigator |
KONDO Noriaki Teikyo University of Science & Technology, Faculty of Science & Engineering, Professor, 理工学部, 教授 (60124343)
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| Project Period (FY) |
2003 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2005: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2004: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2003: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | Action spectrum / Arabidopsis / cis element / Cucumber plant / Induction of gene expression / photolyase / Signal transduction / UV-B / 紫外線耐性 / 日周変化 |
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| Research Abstract |
Growth of cucumber first leaves was retarded by UV-B. It was suggested that light recovery enzyme of DNA damage was concerned with UV tolerance of plants, because the action spectrum for growth retardation was similar to the spectrum for DNA damage caused by light. Thus, I studied to elucidate control system of gene expression of a light recovery enzyme (photolyase). I isolated the cDNA, which encoded CPD-photolyase, and the genome DNA (CsPHR) from cucumber plants The level of transcript (mRNA) of CsPHR reached a maximum in the morning in the photoperiod. The peak of this morning became higher by UV-B addition.
I made the action spectrum of CsPHR induction by light, and the spectrum showed the highest peak of induction around 310nm. Next, I introduced the chimera gene, GUS gene as a reporter bound to the promoter of CsPHR, into Arabidopsis. GUS was expressed in the tip of leaves, the stem and the roots when light including UV-B was irradiated to these transgenic plants. In this case too, the most prominent peak of the induction was observed around 310nm in the action spectrum for GUS induction.
Synthetic derivatives of the promoter harboring a reporter gene LUC were introduced into Arabidopsis, to clarify cis domain of the promoter, and the induction of LUC by UV-B was examined. It was found that a promoter domain of 202-296bp upper stream of the translation start point was a cis domain essential for the induction. However, it was also showed that this domain alone was not possible to induce the reporter, and it was suggested that there are other factors which work cooperatively with the domain within 201bp upper stream of the translation start.
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Report
(4 results)
Research Products
(6 results)
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[Journal Article] Wavelength-dependency of the Light-driven Transcriptional Activation of the Cucumber CPD Photolyase Gene2005
Author(s)
M.Ioki, S.Takahashi, N.Nakajima, H.Saji, K.Fujikura, M.Tamaoki, M.Aono, M.Kanna, D.Ogawa, M.Watanabe, N.Kondo
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Journal Title
Phyton 45・4
Pages: 177-184
Description
「研究成果報告書概要(和文)」より
Related Report
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[Journal Article] Wavelength-dependency of the Light-driven Transcriptional Activation of the Cucumber CPD Photolyase Gene2005
Author(s)
M.Ioki, S.Takahashi, N.Nakajima, H.Saji, K.Fujikura, M.Tamaoki, M.Aono, M.Kanna, D.Ogawa, M.Watanabe, N.Kondo.
-
Journal Title
Phyton 45.4
Pages: 177-184
Description
「研究成果報告書概要(欧文)」より
Related Report
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