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Determination of the spatial arrangement of polypeptides in photosystem II by photo-and oxygen radical-induced protein cross-linking.

Research Project

Project/Area Number 15570036
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物生理・分子
Research InstitutionOKAYAMA UNIVERSITY

Principal Investigator

YAMAMOTO Yasusi  Okayama University, Graduate School of Natural Science and Technology, Professor, 大学院・自然科学研究科, 教授 (40091251)

Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2004: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2003: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsPhotosynthesis / Photosystem II / Protein cross-linking / Reactive oxygen species / Oxygen-evolving system / Polypeptide / Manganese cluster / Protein photodamage / 分子間架橋 / 光ストレス / D1タンパク質 / プロテアーゼ / FtsH / 熱ストレス / 酵素発生系
Research Abstract

The initial purpose of the present research was to identify unknown polypeptides that are located closely to the reaction-center binding D1 protein in photosystem II by the use of chemical cross-linking and light-induced cross-linking methods. However, during the course of the study, we found that a metalloprotease FtsH is located near the core complex of photosystem II. We then have focused our efforts to clarify the roles of the FtsH protease in photosystem II. The following results were obtained.
1.By chemical and photochemical cross-linking experiments, we found that the FtsH protease reacts with the D1 protein. These data as well as the membrane fractionation data strongly suggest that the FtsH protease can recognize the photodamaged D1 protein at the grana thylakoids.
2.By moderate heat treatment (40゜C for 30 min) on spinach thylakoids, the D1 protein was cleaved. We found that the FtsH is responsible for the cleavage of the heat-damaged D1 protein.
3.Using a cyanobacterial mutant lacking the FtsH protease, we confirmed that the the heat-or light-damaged D1 protein in the mutant cells is not removed, and thereby the growth and oxygen-evolving activity are significantly inhibited.
All these results strongly suggest that the FtsH protease is essential for the quality control of photosystem II under light and heat stresses.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (13 results)

All 2005 2004 2003 Other

All Journal Article (11 results) Book (1 results) Publications (1 results)

  • [Journal Article] Quality control of photosystem II under light stress - Turnover of the aggregates of the D1 protein in vivo2005

    • Author(s)
      S.Ohira
    • Journal Title

      Photosynthesis Res.

      Pages: 217-227

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Pressure equilibrium and jump study on unfolding of 23 kDa protein from spinach photosystem II2005

    • Author(s)
      C.Y.Tan
    • Journal Title

      Biophys.J. 88

      Pages: 1264-1275

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Quality control of photosystem II under light stress-Turnover of the aggregates of the D1 protein in vivo2005

    • Author(s)
      S.Ohira
    • Journal Title

      Photosynthesis Res.

      Pages: 217-227

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Quality control of photosystem II under light stress - Turnover of the aggregates of the D1 protein in vivo2005

    • Author(s)
      S.Ohira
    • Journal Title

      Photosynthesis Res. (印刷中)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Isolation and reactive characterization of oxygen-evolving photosystem II complexes retaining the PsbO, P and Q proteins from Euglena gracilis2004

    • Author(s)
      T.Suzuki
    • Journal Title

      Plant Cell Physiol. 45

      Pages: 1168-1175

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Release and reactive oxygen-mediated damage of the oxygen-evolving complex subunits of PS II during photoinhibition2004

    • Author(s)
      T.Henmi
    • Journal Title

      Plant Cell Physiol. 45

      Pages: 243-250

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Isolation of photosystem II -enriched membranes and the oxygen-evolving complex (OEC) subunit proteins from higher plants2004

    • Author(s)
      Y.Yamamoto
    • Journal Title

      Methods in Molecular Biology : Photosynthesis Protocols

      Pages: 29-36

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Assay of photoinhibition of photosystem II and protease activity2004

    • Author(s)
      Y.Yamamoto
    • Journal Title

      Methods in Molecular Biology : Photosynthesis Protocols

      Pages: 217-227

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Isolation and characterization of oxygen-evolving photosystem II complexes retaining the PsbO, P and Q proteins from Euglena gracilis2004

    • Author(s)
      T.Suzuki
    • Journal Title

      Plant Cell Physiol. 45

      Pages: 1168-1175

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Release and reactive oxygen-mediated damage of the oxygen-evolving complex subunits of PSII during photoinhibition2004

    • Author(s)
      T.Henmi
    • Journal Title

      Plant Cell Physiol. 45

      Pages: 243-250

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Dynamic interaction between the D1 protein, CP43 and OEC33 at the lumenal side of photosystem II in spinach chloroplasts : evidence from light induced cross-linking of the proteins in the donor-side photoinhibition2003

    • Author(s)
      T.Henmi
    • Journal Title

      Plant Cell Physiol. 44

      Pages: 451-456

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] Methods in Molecular Biology2004

    • Author(s)
      Y.Yamamoto
    • Publisher
      Humana Press
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] T.Henmi: "Release and reactive-oxygen-mediated damage of the oxygen-evolving complex subunits of PSII during photoinhibition"Plant Cell Physiol.. 45. 243-250 (2004)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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