Reconstitution of lypophilic components in Photosystem I complex which had been extracted with diethyl ether
| Project/Area Number |
15570040
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物生理・分子
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| Research Institution | Teikyo University |
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Principal Investigator |
IKEGAMI Isamu Teikyo Univ., Fac.Pharm.Sci., Prof., 薬学部, 教授 (10082322)
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| Project Period (FY) |
2003 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 2005: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2004: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2003: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | Photosynthesis / Photosystem I / Photosynthetic Reaction center / Chlorophyll / P700 |
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| Research Abstract |
1.Chlorophylls, such as Chl b, Chl a epimer and Phe a, all which do not act as intrinsic antenna in the PSI RC complex, were added with phosphatidylglycerol to the antenna-depleted PSI complex that had a Chl a/P700 ratio of 12. We found that the Chl a-binding site allows to receive Chl b with the same affinity as Chl a, but Chl a epimer with the affinity half of Chl a. The bound Chl b and Chl a epimer act as efficient antenna as Chl a. Phe a binds the antenna-depleted PSI complex, but without any efficient transfer of light energy to P700. We conclude that Chl a-binding site is tolerable to Chl with a side-chain different from Chl a, showing lower affinity with a large structural difference, and that Mg is essential to bind the site.
2.Chlorella, when grown in the dark with an extremely high concentration of Zn, formed Zn-Chl a in amount of about 30% of Chl a. We found that the Zn-Chl a binds to PSI and PSII RC complexes and acts as efficient antenna as Mg-Chl a.
3.PSI complex isolated from phylloquinone-depleted mutant of Arabidopsis, had no P700 but emitted the far red fluorescence at 77K which is characteristic to PSI antenna. We suggest that it had a PSI complex with antenna Chl a but without PSI electron carriers such as P700 and A_0.
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Report
(4 results)
Research Products
(24 results)
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[Journal Article] The energy gap between P700^+-A_0^- and P700^* in photosystem I determined by delayed fluorescence at 270-77K2005
Author(s)
Shibata, Y., Kasahara, T., Akai, S., Itoh, S., Ikegami, I.
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Journal Title
Photosynthesis : Fundamental Aspects to Global Perspectives (eds.A.van der Est, D.Bruce)
Pages: 94-97
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Book] 光合成事典2003
Author(s)
日本光合成研究会編
Total Pages
430
Publisher
学会出版センター
Description
「研究成果報告書概要(和文)」より
Related Report
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