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Neural mechanism underlying re-depression of crayfish tailflip habituation

Research Project

Project/Area Number 15570060
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Animal physiology/Animal behavior
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

NAGAYAMA Toshiki  Hokkaido Univ., Grad.School of Sci., Asso.Prof., 大学院・理学研究科, 助教授 (80218031)

Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2004: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2003: ¥2,300,000 (Direct Cost: ¥2,300,000)
Keywordscrayfish / escape behavior / learning / memory / CNS / neurone / octopamine / serotonin / 馴化 / 再抑圧 / シナプス
Research Abstract

Crayfish escape up and forwards by flipping its tail when intense stimulus is applied to their tailfan. This escape behavior is mediated by a pair of giant fiber, lateral giants (LGs). The LGs receive mechanosensory information via direct electrical and chemical synapses from the sensory afferents and disynaptically via ascending interneurones. Bath application of serotonin (5-HT) increased the EPSP amplitude of LGs in response to the sensory stimulation though their resting potential and membrane resistance remained consistent. To analyze the nature of 5-HT modulation, sensory responses of LG escape circuit neurones were analyzed under the mixture of membrane permeable cAMP analog, CPT-cAMP and cAMP phosphodiesterase inhibitor IBMX. As 5-HT superfusion, EPSP amplitude of LGs was increased by cAMP analog superfusion. The increase of response of LGs was reduced after about 30 min of washing with normal saline. The sensory responses of interneurones A and C also increased by cAMP analog. These results suggest 5-HT increases intrinsic level of cAMP in LG escape circuit.
Bath application of biogic amines, serotonin (5-HT) or octopamine (OA) increased amplitude of LG EPSPs in response to sensory stimulation. To investigate the action of these biogic amines, LG activity was analyzed under the mixture of membrane permeable cAMP analogue (CPT-cAMP) and cAMP phosphodiesterase inhibitor (IBMX) or cGMP analogue (8-Br-cGMP) alone. The cAMP analogue increased LG activity while cGMP analogue had no effect. Adenylate cyclase inhibitor SQ22536 inhibited the effect of 5-HT but had no effect on OA. Iontophoretic injection of cAMP analogue, Sp-cAMP, directly into LG mimicked the modulatory effect of 5-HT. These results suggest 5-HT increases LG activity by promoting intrinsic level of cAMP in LG itself.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (24 results)

All 2005 2004 2003 Other

All Journal Article (19 results) Publications (5 results)

  • [Journal Article] Decrease in excitability of LG following habituation of the crayfish escape reaction.2005

    • Author(s)
      Araki M.
    • Journal Title

      J.Comp.Physiol.A (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] GABAergic and glutamatergic inhibition of the nonspiking local interneurones in the terminal abdominal ganglion of the crayfish.2005

    • Author(s)
      Nagayama T.
    • Journal Title

      J.Exp.Zool. 303A

      Pages: 66-75

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Decrease in excitability of LG following habituation of the crayfish escape reaction.2005

    • Author(s)
      Araki M, Nagayama T.
    • Journal Title

      J.Comp.Physiol.A (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] GABAergic and glutamatergic inhibition of the nonspiking local interneurones in the terminal abdominal ganglion of the crayfish2005

    • Author(s)
      Nagayama T.
    • Journal Title

      J.Exp.Zool. 303A

      Pages: 66-75

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Decrease in excitability of LG following habituation of the crayfish escape reaction.2005

    • Author(s)
      Araki M., Nagayama T.
    • Journal Title

      J.Comp.Physiol.A (in press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Synaptic interactions between nonspiking local interneurones in the terminal abdominal ganglion of the crayfish.2004

    • Author(s)
      Namba H.
    • Journal Title

      J.Comp.Physiol.A 190

      Pages: 615-622

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Distribution of glutamatergic immunoreactive neurons in the terminal abdominal ganglion of the crayfish.2004

    • Author(s)
      Nagayama T.
    • Journal Title

      J.Comp.Neurol. 474

      Pages: 123-135

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Nitric oxide modulates local reflexes of the tailfan of the crayfish.2004

    • Author(s)
      Araki M.
    • Journal Title

      J.Neurobiol. 60

      Pages: 176-186

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effects of nitric oxide on proprioceptive signaling.2004

    • Author(s)
      Schuppe H.
    • Journal Title

      Zool.Sci. 21

      Pages: 1-5

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Synaptic interactions between nonspiking local interneurones in the terminal abdominal ganglion of the crayfish.2004

    • Author(s)
      Namba H, Nagayama T.
    • Journal Title

      J.Comp.Physiol.A 190

      Pages: 615-622

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Distribution of glutamatergic immunoreactive neurons in the terminal abdominal ganglion of the crayfish.2004

    • Author(s)
      Nagayama T., Kimura K., Araki M., Aonuma H, Newland P.L.
    • Journal Title

      J.Comp.Neurol. 474

      Pages: 123-135

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Nitric oxide modulates local reflexes of the tailfan of the crayfish.2004

    • Author(s)
      Araki M., Schuppe H., Fujimoto S., Nagayama T, Newland P.L.
    • Journal Title

      J.Neurobiol. 60

      Pages: 176-186

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effects of nitric oxide on proprioceptive signaling.2004

    • Author(s)
      Schuppe H., Araki M., Aonuma H., Nagayama T, Newland P.L.
    • Journal Title

      Zool.Sci. 21

      Pages: 1-5

    • NAID

      110006281183

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effects of nitric oxide on proprioceptive signaling2004

    • Author(s)
      Schuppe H. et al.
    • Journal Title

      Zool.Sci. 21

      Pages: 1-5

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Nitric oxide modulates local reflexes of the tailfan of the crayfish2004

    • Author(s)
      Araki M. et al.
    • Journal Title

      J.Neurobiol. 60

      Pages: 176-186

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Distribution of glutamatergic immunoreactive neurons in the terminal abdominal ganglion of the crayfish.2004

    • Author(s)
      Nagayama T. et al.
    • Journal Title

      J.Comp.Neurol. 474

      Pages: 123-135

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Synaptic interactions between nonspiking local interneurones in the terminal abdominal ganglion of the crayfish.2004

    • Author(s)
      Namba H., Nagayama T.
    • Journal Title

      J.Comp.Physiol.A 190

      Pages: 615-622

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Direct chemically mediated synaptic transmission from mechanosensory afferents contributes to habituation of crayfish lateral giant escape reaction.2003

    • Author(s)
      Araki M.
    • Journal Title

      J.Comp.Physiol.A 189

      Pages: 731-739

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Direct chemically mediated synaptic transmission from mechanosensory afferents contributes to habituation of crayfish lateral giant escape reaction.2003

    • Author(s)
      Araki M, Nagayama T.
    • Journal Title

      J.Comp.Physiol.A 189

      Pages: 731-739

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Araki M, Nagayama T: "Direct chemically mediated synaptic transmission from mechanosensory afferents contributes to habituation of crayfish lateral giant escape reaction"J Comp Physiol A. 189. 731-739 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Schuppe H et al.: "Effects of nitric oxide on proprioceptive signaling"Zool Sci. 21. 1-5 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Araki M et al.: "Nitric oxide modulates local reflexes of the tailfan of the crayfish"J Neurobiol. (in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Nagayama T et al.: "Distribution of glutamaterigic immunoreactive neurons in the terminal abdominal ganglion of the crayfish"J Comp Neurol. (in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Namba H, Nagayama T: "Synaptic interactions between nonspiking local internerurones in the terminal abdominal ganglion of the crayfish"J Comp Physiol A. (in press). (2004)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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