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Neural circuit regulating drinking behavior in the eel acclimated to sea water

Research Project

Project/Area Number 15570064
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Animal physiology/Animal behavior
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

ANDO Masaaki  Hiroshima Univ., Faculty of Integrated Arts and Sciences, Professor, 総合科学部, 教授 (10100976)

Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2004: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2003: ¥2,400,000 (Direct Cost: ¥2,400,000)
Keywordsthirst / circumventricular organs / vasotocin / catecholamine(s) / upper esophageal sphincter muscle / vagal branches / glossopharyngeal-vagal motor complex / vagal lobe / 飲水行動 / 間脳 / 延髄 / 舌咽・迷走運動核(GVC) / 迷走神経葉(LX) / 細胞内Ca^<2+>濃度 / 神経活動 / 受容体 / Antagonists / Iontophoretic injection
Research Abstract

Although the circumventricular organs(CVOs) in the eel is not identified yet, we identified 3 CVOs in the eel, magnocellular preoptic nucleus (PM), anterior tuberal nucleus (NAT) and area postrema (AP). The PM and the AP were immuno- reactive to vaosotocin (AVT)- and tyrosine hydroxylase (TH)-antibody, respectively (Mukuda et al., 2005).
Swallowing is due to relaxation of the upper esophageal sphincter(UES) muscle. After injection of Evans blue into the UES, the glossopharyngeal-vagal motor complex (GVC) was labeled retrogradely, and these neurons were immunoreactive to choline acetyltransfetase-antibody (Mukuda & Ando, 2003). Indeed, the UES muscle (striated muscle) was innervated by a cholinergic neuron and constricted by ACh (Kozaka & Ando, 2003). The vagus nerve was divided to 10 branches (X1-X10) behind the gill arcs. Among these vagal branches, only X5 situated on the internal jugular vain innervated the UES, with optimal frequency of 20 Hz (Ogawa & Ando, in preparation). The existence of optimum frequency seems to be due to Ca^<2+>-ATPase on the sarcoplasmic reticulum (Tamura & Ando, in preparation). On the other hand, the UES was constricted by AVT and relaxed by isotocin via cAMP (Watanabe & Ando, in preparation).
Neuronal activity of the GVC was inhibited by catecholamines in a dose-dependent manner, and this inhibitory effects were blocked by yohimbine. Since the vagal lobe (LX), the commissural nucleus of Cajal (NCC), the reticular formation (RF) and the AP in the medulla are immunoreactive to TH-antibody, some of these nuclei may innervate the GVC (Ito, Mukuda & Ando, submitted). Indeed, the GVC activity was inhibited by an electrical stimulation on the LX, but the inhibitory effect was not blocked by yohimbine (Ito & Ando, in preparation). Therefore, it is likely that the GVC activity is regulated by many inputs and the neuronal circuit on the GVC seems to be complicated.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (22 results)

All 2005 2003 Other

All Journal Article (13 results) Book (3 results) Publications (6 results)

  • [Journal Article] "Blood-contacting neurons" in the brain of the Japanese eel Anguilla japonica2005

    • Author(s)
      Mukuda, Matsunaga, Kawamoto, Yamaguchi, Ando
    • Journal Title

      J.Exp.Zool. 303A

      Pages: 366-376

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] "Blood-contacting neurons" in the brain of the Japanese eel Anguilla japonica2005

    • Author(s)
      Mukuda, T., Matsunaga, Y, Kawamoto, K., Yamaguchi, K., Ando, M
    • Journal Title

      J.Exp.Zool. 303A

      Pages: 366-376

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] "Blood-contacting neurons" in the brain of the Japanese eel Anguilla japonica2005

    • Author(s)
      Mukuda, Matsunaga, Kawamoto, Yamaguchi, Ando
    • Journal Title

      J.Exp.Zool. (in press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Medullary motor neurons associated with drinking behaviour of Japanese eels2003

    • Author(s)
      Mukuda, Ando
    • Journal Title

      J.Fish Biol. 62

      Pages: 1-12

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Cholinergic innervation to the upper esophageal sphincter muscle in the eel, with special reference to drinking behavior2003

    • Author(s)
      Kozaka, Ando
    • Journal Title

      J Comp.Physiol. B173

      Pages: 135-140

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Central effects of various ligands on drinking behavior in eels acclimated to sea water2003

    • Author(s)
      Kozaka, Fujii, Ando
    • Journal Title

      J.Exp.Biol. 206

      Pages: 687-692

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Brain atlas of the Japanese eel : comparison to other fishes2003

    • Author(s)
      Mukuda, Ando
    • Journal Title

      Science Reports, Mem.Fac. Integrated Arts & Sci., Hiroshima Univ.Ser.IV 29

      Pages: 1-25

    • NAID

      120000881241

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Water metabolism in the eel acclimated to sea water : from mouth to intestine2003

    • Author(s)
      Ando, Mukuda, Kozaka
    • Journal Title

      Comp.Biochem.Physiol. 136B

      Pages: 621-633

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Medullary motor neurons associated with drinking behaviour of Japanese eels2003

    • Author(s)
      Mukuda, T., Ando, M.
    • Journal Title

      J.Fish Biol. 62

      Pages: 1-12

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Cholinergic innervation to the upper esophageal sphincter muscle in the eel, with special reference to drinking behavior2003

    • Author(s)
      Kozaka, T., Ando, M
    • Journal Title

      J.Comp.Physiol.B 173

      Pages: 135-140

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Central effects of various ligands on drinking behavior in eels acclimated to sea water2003

    • Author(s)
      Kozaka, T., Fujii, Y., Ando, M
    • Journal Title

      J.Exp.Biol. 206

      Pages: 687-692

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Brain atlas of the Japanese eel : comparison to other fishes2003

    • Author(s)
      Mukuda, T., Ando, M
    • Journal Title

      Science Reports, Mem.Fac.Integrated Arts & Sci., Hiroshima Univ.Ser.IV Vol.29

      Pages: 1-25

    • NAID

      120000881241

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Water metabolism in the eel acclimated to sea water : from mouth to intestine2003

    • Author(s)
      Ando, M., Mukuda, T., Kozaka, T
    • Journal Title

      Comp.Biochem.Physiol. 136B

      Pages: 621-633

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] 大学新入生に進める101冊の本2005

    • Author(s)
      難波紘二 他
    • Total Pages
      266
    • Publisher
      岩波書店
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] 101 books for freshmen (Japanese)2005

    • Author(s)
      Ando, M
    • Publisher
      Iwanami-shoten
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] 大学生に薦める101冊の本2005

    • Author(s)
      難波紘二 他
    • Total Pages
      256
    • Publisher
      岩波書店
    • Related Report
      2004 Annual Research Report
  • [Publications] Mukuda, Ando: "Medullary motor neurons associated with drinking behaviour of Japanese eel"J.Fish Biol.. 62. 1-12 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kozaka, Ando: "Central effects of various ligands on drinking behavior in eels acclimated to sea water"J.Exp.Biol.. 206. 687-692 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kozaka, Ando: "Cholinergic innervation to upper esophageal sphincter muscle in the eel, with special reference to drinking behavior"J.Comp.Physiol.. B173. 135-140 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Mukuda, Ando: "Brain atlas of the Japanese eel : comparison to other fishes"Mem.Fac.Integrated Arts and Sci, Hiroshima Univ., Ser. IV. 29. 1-25 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Sakamot, Fujimoto, Ando: "Fishy tales of prolactin-releasing peptide"Int.Rev.Cytol.. 225. 91-130 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ando, Mukuda, Kozaka: "Water metabolism in the eel acclimated to sea water : from mouth to intestine"Comp.Biochem.Physiol.Part B. 136. 621-633 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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