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Phylogenetic study of diatoms based on kinetic morphology formed during valve ontogeny and molecular associated with bio-silica mineralization

Research Project

Project/Area Number 15570075
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biodiversity/Systematics
Research InstitutionTokyo Gakugei University

Principal Investigator

MAYAMA Shigeki  Tokyo Gakugei Univ., Education, Associated Professor, 教育学部, 助教授 (40199914)

Co-Investigator(Kenkyū-buntansha) OKANO Keijyu  Akita Pref.Univ., Bioresource science, Associated Professor, 生物資源科学部, 助教授 (40147070)
NAGUMO Tamotsu  Nihon Dental Univ., Dentistry, Professor, 歯学部, 教授 (70120706)
IDEI Masahiko  Bunkyo University, Education, Professor, 教育学部, 教授 (60143624)
Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2005: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2004: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2003: ¥1,500,000 (Direct Cost: ¥1,500,000)
Keywordsdiatoms / valve ontogeny / silaffin / LCPA / Melosira / Phylogeny / polyamin / silica nano-pattern / 殻形態形成 / 系統発生 / 動的形態 / 系統学 / シリカミネラリゼーション / Pinnularia / Caloneis / 被殻 / Cylindrotheca / 新種 / シラフィン / sil1 / 分子系統
Research Abstract

Optimal method for extructing organic matters from valve inside of two Melosira, Biddulphia was studied. SDS-PAGE showed that two Merosira species did not include silaffin but had matter, which appeared by CBB staining below the popsition of 3.5kDa. Whereas Biddulphia showed CBB band positioned between 31-36.5kDa and also below 3.5kDa. The matter obtained from Melosira make sediment by mixing with silicic acid solution imediately. EDS analysis indicated that the sediment matter containig Si and O. The sediment was compposed of many granula. The sizes were different between two Melosira species, but it changed by addition of phosphate acid, 25 to 400nm. Pureified matter by HPLC contained a fraction which showed strong reaction with Ninhydryn. Sedimentation assay with this fraction generated silica-nanosphere, ca. 50nm in diameter. Control with commercial product of polyamin showed similar silica-nanosphere, so that the matter in the flaction was supposed to be LCPA. In addition, some othere flaction also made sediments, and some of them showed mesh-like structure, which was quite similar to that of real Melosira valve.
Pennate Caloneis bacillum was cultered and proceedure of its valve development was clarified by SEM observations. The result showed that after complete development of virgae, their apices were fused together and marginal ring of silica was formed.

Report

(4 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (22 results)

All 2006 2005 2004 2003 Other

All Journal Article (14 results) Book (3 results) Publications (5 results)

  • [Journal Article] Phylogenetic position of the small solitary phaeodarians (Radiolaria) based on 18S rDNA sequences by single cell PCR analysis.2006

    • Author(s)
      Yuasa, T., Takahashi, O., Dolven, J.K., Mayama, S., Matsuoka, A., Honda, D., Bjorklund, K.R.
    • Journal Title

      Marine Micropaleontology 59(in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Phylogenetic position of the small solitary pghaeodarians (Radiolaria) based on 18S rDNA sequences by single cell PCR analysis.2006

    • Author(s)
      Yuasa, T., Takahashi, O., Dolven, J.K., Mayama, S., Matsuoka, A., Honda, D., Bjorklund, K.R.
    • Journal Title

      Marine Micropaleontology 59

      Pages: 104-114

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Phylogenetic analyses of the polycystine Radiolaria based on the 18s rDNA sequences of the Spumellarida and the Nassellarida.2005

    • Author(s)
      Yuasa, T., Takahashi, O., Honda, D., Mayama, S.
    • Journal Title

      Eur. J. Protistology 41

      Pages: 287-298

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] A new marine diatom Cocconeis sagaraensis Hid. Suzuki (Bacilla riophyceae) from Japan2005

    • Author(s)
      Suzuki, H., Nagumo, T., Tanaka, J.
    • Journal Title

      J. Jpn. Bot. 80

      Pages: 176-185

    • NAID

      40006823315

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Fine structure of the vefetative frustule, perizonium and initial valve of Achnanthes yaquinensis (Bacillariophyta)2005

    • Author(s)
      Toyoda, K., Idei, T., Nagumo, Tanaka, J.
    • Journal Title

      Eur. J. Phycol. 40

      Pages: 269-279

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Phylogenetic analyses of the polycystine Radiolaria based on the 18s rDNA sequences of the Spumellarida and the Nassellarida.2005

    • Author(s)
      Yuasa, T., Takahashi, O., Honda, D., Mayama, S.
    • Journal Title

      Eur.J.Protistology 41

      Pages: 287-298

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] A new marine diatom Cocconeis sagaraensis Hid. Suzuki (Bacillariophyceae) from Japan2005

    • Author(s)
      Suzuki, H., Nagumo, T., Tanaka, J.
    • Journal Title

      J.Jpn.Bot 80

      Pages: 176-185

    • NAID

      40006823315

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Fine structure of the vefetative frustule, perizonium and initial valve of Achnanthes yaquinensis (Bacillariophyta)2005

    • Author(s)
      Toyoda, K., M.Idei, T.Nagumo, Tanaka, J.
    • Journal Title

      Eur.J.Phycol. 40

      Pages: 269-279

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Mechanism of glass shell formation of diatoms.2005

    • Author(s)
      Okano, K., Mayama, S.
    • Journal Title

      Function of marine organisms : How they adapt to diverse marine environments? (Takei, Y.(ed.))(Tokai University Press.)

      Pages: 428-428

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] A new marine diatom Cocconeis sagaraensis Hid.Suzuki (Bacillariophyceae) from Japan2005

    • Author(s)
      Suzuki, H., Nagumo, T., Tanaka, J.
    • Journal Title

      J.Jpn.Bot. 80

      Pages: 176-185

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Characterization of linear-oblong pyrenoids with cp-DNA along their sides in Nitzschia sigmoidea (Bacillariophyceae).2004

    • Author(s)
      Mayama, S., Mayama, N., Shihira-Ishikawa, I.
    • Journal Title

      Phycologcal Research 52

      Pages: 129-139

    • NAID

      10013282453

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Characterization of linear-oblong pyrenoids with cp-DNA a long, their sides in Nitzschia sigmoidea (Bacillariophyceae).2004

    • Author(s)
      Mayama, S., Mayama, N., Shihira-Ishikawa, I.
    • Journal Title

      Pycological Research 52・2

      Pages: 129-139

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Molecular phylogeny of solitary shell-bearing Polycystinea (Radiolaria).2004

    • Author(s)
      Takahashi, O., Yuasa, T., Honda, D., Mayama, S.
    • Journal Title

      Revue de micropaleontologie 47

      Pages: 111-118

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Observations of two new species of Navicula : N.exiloides and N.delicatilineolata.2003

    • Author(s)
      Mayama, S.
    • Journal Title

      Diatom 19

      Pages: 17-22

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] 「珪藻のガラス骨格形成機構」海洋生物の機能 海洋生命系のダイナミクス第2巻(竹井祥郎編)2005

    • Author(s)
      岡野桂樹, 真山茂樹
    • Total Pages
      428
    • Publisher
      東海大学出版会
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] 「珪藻のガラス骨格形成機構」海洋生物の機能海洋生命系のダイナミクス第2巻(竹井祥郎 編)2005

    • Author(s)
      岡野桂樹, 真山茂樹
    • Total Pages
      428
    • Publisher
      東海大学出版会
    • Related Report
      2005 Annual Research Report
  • [Book] 海洋生態系のダイナミクスシリーズ第2巻第7章 珪藻のガラス骨格形成機構2005

    • Author(s)
      岡野桂樹, 真山茂樹
    • Total Pages
      17
    • Publisher
      東海大学出版会
    • Related Report
      2004 Annual Research Report
  • [Publications] Mayama, S.: "Observations of two new species of Navicula : N.exiloides and N.delicatilineolata."Diatom. 19・1. 17-22 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Mayama, S., Mayama, N.Shihira-Ishikawa, I.: "Characterization of linear-oblong pyrenoids with cp-DNA along their aides in Nitzschia sigmoidea (Bacillariophyceae)."Phycological Research. 25・2(印刷中). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Takahashi, O., Mayama, S., Matsuoka, A.: "Host-symbiont associations of polycystine Radiolaria epifluorescence microscopic observation of living Radiolaria."Marin Micropaleontology. 49. 187-194 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Nagumo, T.: "Taxonomic studies of the subgenus Amphora Cleve of the genus Amphora (Bacillariophyceae) in Japan."Bibliotheca Diatomologica. 49. 1-65 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Toyoda, K., Nagumo, T., Osada, K., Tanaka, J.: "Morphological investigations of Achnanthes javanica Grunow and A.subconstricta (Meister) comb.nov."Diatom Research. 18・2. 365-375 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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