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Molecular mechanism of stop codon recognition by eRF1

Research Project

Project/Area Number 15570104
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Structural biochemistry
Research InstitutionRIKEN (2004)
National Cancer Center Research Institute and Research Center for Innovative Oncology, National Cancer Center Hospital East (2003)

Principal Investigator

MURAMATSU Tomonari  RIKEN, Protein Structure Team, Senior Research Scientist, タンパク質構造研究チーム, 上級研究員 (70212256)

Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2004: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2003: ¥1,600,000 (Direct Cost: ¥1,600,000)
Keywordseukaryote / protein biosynthesis / stop codon / codon recognition / translation termination / eRF1 / human
Research Abstract

ETF1/eRF1,which has recently been suggested to be a myeloid tumor suppressor gene, codes for the class-I release factor which recognizes an in-frame stop codon in translation termination processes of gene expression. This proteinaceous factor eRF1(eukaryotic release factor 1) recognizes all of the three stop codons, UAA, UGA and UAG, but not the Trp codon UGG. This discrimination must require some conformational changes in the recognition domain of eRF1,because the UGG codon cannot be eliminated if eRF1 recognizes the stop codons by simply binding to A/G as the 2nd letter and A/G the 3rd. To address the mechanism of stop codon recognition, we prepared ^<15>N-labeled and ^<15>N/^<13>C-labeled codon recognition domains, assigned all ^1H^N and ^<15>N resonances of native non-proline back bone residues, and now are analyzing dynamic structure of this domain by NMR spectroscopy.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (10 results)

All 2004 2003 Other

All Journal Article (8 results) Publications (2 results)

  • [Journal Article] Identification of arginine residues important for the activity of Escherichia coli signal Peptidase I2004

    • Author(s)
      Yong-Tae Kim et al.
    • Journal Title

      Biological Chemistry 385

      Pages: 381-388

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Analysis of dynamic structure of eukaryotic release factor 1 (eRF1)2004

    • Author(s)
      Tomonari Muramatsu, et al.
    • Journal Title

      Bioimades 12

      Pages: 37-37

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Backbone ^1H, ^<13>C and ^<15>N resonance assignment of the N-terminal domain of human eRF12004

    • Author(s)
      Yoshifumi Oda, et al.
    • Journal Title

      Journal of Biomolecular NMR 30

      Pages: 109-110

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] The three-dimensional structure of aspergilloglutamic peptidase from Aspergillus niger2004

    • Author(s)
      Hiroshi Sasaki, et al.
    • Journal Title

      Proceedings of Japan Academy 80(B)

      Pages: 435-438

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Identification of arginine residues important for the activity of Escherichia coli signal peptidase I2004

    • Author(s)
      Yong-Tae Kim, et al.
    • Journal Title

      Biological Chemistry 385

      Pages: 381-388

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Analysis of dynamic structure of eukaryotic release factor 1(eRF1)2004

    • Author(s)
      Tomonari Muramatsu, et al.
    • Journal Title

      Bioimages 12

      Pages: 37-37

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Molecular mechanism of stop codon recognition by eukaryotic relase factor 1 (eRF1)2003

    • Author(s)
      Tomonari Muramatsu, et al.
    • Journal Title

      Biomages 11

      Pages: 44-44

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Molecular mechanism of stop codon recognition by eukaryotic relase factor 1(eRF1)2003

    • Author(s)
      Tomonari Muramatsu, et al.
    • Journal Title

      Bioimages 11

      Pages: 44-44

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Tomonari Muramatsu, Yoshifumi Oda, Fumiaki Yumoto, Mie Ito, Masaru Tanokura: "Molecular Mechanism of Stop Codon Recognition by Eukaryotic Release Factor 1 (eRF1)"Bioimages. 11. 44-44 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yoshifumi Oda, Tomonari Muramatsu, Fumiaki Yumoto, Mie Ito, Masaru Tanokura: "Backbone ^1H, ^<13>C and ^<15>N resonance assignment of the N-terminal domain of human eRF1"Journal of Biomolecular NMR. 印刷中. (2004)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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