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Quinones in the Photosynthetic Reaction Center of Green Sulfur Bacteria

Research Project

Project/Area Number 15570135
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biophysics
Research InstitutionOsaka University

Principal Investigator

OH-OKA Hirozo  Osaka University, Graduate School of Science, Associate Professor, 理学研究科, 助教授 (30201966)

Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2004: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2003: ¥2,300,000 (Direct Cost: ¥2,300,000)
KeywordsGreen Sulfur Bacteria / Photochemical Reaction Center / Electron Transfer / Energy Transfer / NADP^+ Photoredution / Cytochrome c / bc complex / P840 / チトクロムc_z / チトクロムc-554 / チオ硫酸酸化経路 / イオウ代謝 / 反応中心
Research Abstract

Analyses with molecular genetic methods are essential for the study of the electron transfer mechanisms in the photosynthetic reaction center (RC) of green sulfur bacteria on the molecular level. We have therefore made a project concerning a mutant generation with the homologous recombination method which was established in 2001. 1. Analysis of the function of PscD subunit : In order to clarify the unknown function of PscD subunit, which is one of 5 different subunits constituting the RC complex, the mutant lacking PscD subunit was generated by the insertional inactivation of the relevant gene pscD. Although the mutant could grow photosynthetically, the energy transfer from FMO protein to P840 was less efficient compared to the wild type. Activity of NADP^+ photoreduction also declined slightly to about 80%. 2. Analysis of the function of cytochrome c-554 : We performed the flash-excitation experiments using membranes purified from the mutant lacking cytochrome c-554. It was demonstrated that cytochrome cz mediated directly the electron transfer from be complex to P840. Furthermore, cytochrome c-554 could not also accept electrons from bc complex. Two different electron transfer pathways to P840 seems to be functional, that is, one is from bc complex and the other from cytochrome c-554 which is linked with a sulfur oxidation pathway. In the near future, this project will be developed to explore quinones which is supposed to exist within the Chlorobium RC and heliobacterial RC as well.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (14 results)

All 2005 2004 2003 Other

All Journal Article (11 results) Publications (3 results)

  • [Journal Article] Different sensitivities to oxygen between two strains of the photosynthetic green sulfur bacterium Chlorobium vibrioforme NCIB 8327 with bacteriochiorophyll c and d.2005

    • Author(s)
      J.Harada
    • Journal Title

      Photosynthesis Research (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Determination of stereochemistry of bacteriochlorophyll-g_F and 8^1-hydroxy-chlorophyll-a_F from Heliobacterium modesticaldum2005

    • Author(s)
      T.Mizoguchi
    • Journal Title

      Photochem.Photobiol. (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] In vitro activity of C-20 methyltransferase, BchU, involved in bacteriochlorophyll c biosynthetic pathway in green sulfur bacteria2005

    • Author(s)
      J.Harada
    • Journal Title

      FEBS Lett. 579

      Pages: 1983-1987

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Different sensitivities to oxygen between two strains of the photosynthetic green sulfur bacterium Chlorobium vibrioforme NCIB 8327 with bacteriochlorophyll c and d2005

    • Author(s)
      J.Harada
    • Journal Title

      Photosynthesis Research (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Determination of stereochemistry of bacteriochlorophyll-g_F and 8^1-hydroxy-chlorophyll-a_F from Heliobacterium modesticaldum2005

    • Author(s)
      T.Mizoguchi
    • Journal Title

      Photochem.Photobiol. in press

    • Related Report
      2004 Annual Research Report
  • [Journal Article] In vitro activity of C-20 methyltransferase, BchU, involved in bacteriochlorophyll c biosynthetic pathway in green sulfur bacteria2005

    • Author(s)
      J.Harada
    • Journal Title

      FEBS Lett. in press

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Function of a PscD subunit in a homodimeric reaction center complex of the photosynthetic green sulfur bacterium Chlorobium tepidum studied by insertional gene inactivation2004

    • Author(s)
      Y.Tsukatani
    • Journal Title

      Journal of Biological Chemistry 279

      Pages: 51122-51130

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Green Bacteria : Secondary Electron Donor (Cytochromes)2004

    • Author(s)
      H.Oh-oka
    • Journal Title

      Encyclopedia of Biological Chemistry Vol.2

      Pages: 521-524

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Presence of exclusively bacteriochlorphyll-c containing substrain in the culture of green sulfur photosynthetic bacterium Chlorobium vibrioforme strain NCIB 8327 producing bacteriochlorophyll-d2003

    • Author(s)
      Y.Saga
    • Journal Title

      Anal.Sci. 19

      Pages: 1575-1579

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Novel carotenoid glucoside esters from alkaliphulic heliobacteria2003

    • Author(s)
      S.Takaichi
    • Journal Title

      Arch Microbiol. 179

      Pages: 95-100

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Novel carotenoid glucoside esters from alkaliphilic heliobacteria2003

    • Author(s)
      S.Takaichi
    • Journal Title

      Arch Microbiol. 179

      Pages: 95-100

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] H.Oh-oka: "Green Bacteria : Secondary Electron Donor (Cytochromes)"Encyclopedia of Biological Chemistry. in press. (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Y.Saga: "Presence of exclusively bacteriochlorphyll-c containing substrain in the culture of green sulfur photosynthetic bacterium Chlorobium vibrioforme strain NCIB 8327 producing bacteriochlorophyll-d"Anal.Sci.. 19. 1575-1579 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] S.Takaichi: "Novel carotenoid glucoside esters from alkaliphilic heliobacteria"Arch Microbiol.. 179. 95-100 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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