Imaging analysis of influenza virus behavior
| Project/Area Number |
15570140
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | Kawasaki Medical School |
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Principal Investigator |
SAKAI Tatsuya Kawasaki Medical School, Medicine, Faculty Assistant, 医学部, 助手 (00309543)
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| Co-Investigator(Kenkyū-buntansha) |
OHUCHI Masanobu Kawasaki Medical School, Medicine, Professor, 医学部, 教授 (80107185)
HIRATA Yoshiki National Institute of Advanced Industrial Science and Technology, Biological Resource and Function, Senior Researcher, 生物機能工学研究部門, 主任研究員 (10357858)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 2004: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2003: ¥3,200,000 (Direct Cost: ¥3,200,000)
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| Keywords | influenza virus / behavioral science / profiling / imaging / emerging virus / ウイルス行動科学 / 感染行動 / 可視化解析 |
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| Research Abstract |
Influenza viruses should be endocytosed to infect host cells. Since endocytic processes occur in specialized regions of a cell surface such as the coated pits, influenza viruses that attach to cell surfaces need to move to the endocytic regions. However, the virus dose not have any specific machinery to move. How does the virus access to the endocytic regions? We hypothesized that the virus particle moves step by step on a cell surface by exchanging bindings between viral hemagglutinin (HA) and its receptor, sialoglycan. To demonstrate the viral motility, we monitored a fluorescence-labeled influenza virus MAC11 reassortant (H3N2) on a glass coverslip coated with fetuin to mimic a cell surface, using a total internal reflection fluorescence microscope. The viruses moved discontinuously on the surface with various steps of 0.2-1.5μm. Moreover, neuraminidase inhibitor (zanamivir) prevented the virus moving. From these results, we conclude that an influenza virus has a potential motility on a surface containing the virus receptors and that NA is essential for the virus to move.
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Report
(3 results)
Research Products
(15 results)
