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Imaging analysis of influenza virus behavior

Research Project

Project/Area Number 15570140
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biophysics
Research InstitutionKawasaki Medical School

Principal Investigator

SAKAI Tatsuya  Kawasaki Medical School, Medicine, Faculty Assistant, 医学部, 助手 (00309543)

Co-Investigator(Kenkyū-buntansha) OHUCHI Masanobu  Kawasaki Medical School, Medicine, Professor, 医学部, 教授 (80107185)
HIRATA Yoshiki  National Institute of Advanced Industrial Science and Technology, Biological Resource and Function, Senior Researcher, 生物機能工学研究部門, 主任研究員 (10357858)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 2004: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2003: ¥3,200,000 (Direct Cost: ¥3,200,000)
Keywordsinfluenza virus / behavioral science / profiling / imaging / emerging virus / ウイルス行動科学 / 感染行動 / 可視化解析
Research Abstract

Influenza viruses should be endocytosed to infect host cells. Since endocytic processes occur in specialized regions of a cell surface such as the coated pits, influenza viruses that attach to cell surfaces need to move to the endocytic regions. However, the virus dose not have any specific machinery to move. How does the virus access to the endocytic regions? We hypothesized that the virus particle moves step by step on a cell surface by exchanging bindings between viral hemagglutinin (HA) and its receptor, sialoglycan. To demonstrate the viral motility, we monitored a fluorescence-labeled influenza virus MAC11 reassortant (H3N2) on a glass coverslip coated with fetuin to mimic a cell surface, using a total internal reflection fluorescence microscope. The viruses moved discontinuously on the surface with various steps of 0.2-1.5μm. Moreover, neuraminidase inhibitor (zanamivir) prevented the virus moving. From these results, we conclude that an influenza virus has a potential motility on a surface containing the virus receptors and that NA is essential for the virus to move.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (15 results)

All 2005 2004 2003 Other

All Journal Article (10 results) Book (2 results) Publications (3 results)

  • [Journal Article] インフルエンザウイルス感染行動の解析2005

    • Author(s)
      堺 立也
    • Journal Title

      細胞 印刷中

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] インフルエンザウイルス感染行動の解析2005

    • Author(s)
      堺 立也
    • Journal Title

      細胞 (印刷中)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Molecular sorting in early endosomes.2004

    • Author(s)
      Tatsuya Sakai.
    • Journal Title

      Kawasaki Medical Journal 30

      Pages: 1-8

    • NAID

      110001789233

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Molecular sorting in early endosomes.2004

    • Author(s)
      Tatsuya Sakai
    • Journal Title

      Kawasaki Medical Journal. 30

      Pages: 1-8

    • NAID

      110001789233

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The role of M cells of human nasopharyngeal lymphoid tissue in influenza virus sampling.2004

    • Author(s)
      Yoshinori Fujimura, Masaharu Takeda, Hidenori Ikai, Ken Haruma, Takeshi Akisada, Tamotsu Harada, Tatsuya Sakai, Masanobu Ohuchi
    • Journal Title

      Virchows Arch. 444

      Pages: 36-42

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Molecular sorting in early endosomes.2004

    • Author(s)
      Tatsuya Sakai
    • Journal Title

      Kawasaki medical Journal 30

      Pages: 1-8

    • NAID

      110001789233

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 宿主細胞表面でのインフルエンザウイルスのスライディング2003

    • Author(s)
      堺 立也
    • Journal Title

      日本臨床 61

      Pages: 1860-1863

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] The role of M cells of human nasopharyngeal lymphoid tissue in influenza virus sampling.2003

    • Author(s)
      Yoshinori Fujimura
    • Journal Title

      Virchows Arch. 444

      Pages: 36-42

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Difference-from-prediction imaging for high resolution shape analysis of locomoting cells.2003

    • Author(s)
      Takafumi Mizuno
    • Journal Title

      Bioimages 11

      Pages: 1-66

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Difference-from-prediction imaging for high resolution shape analysis of locomoting cells.2003

    • Author(s)
      Takafumi Mizuno, Tatsuya Sakai, Kyoko Yoshioka, Kazunori Kawasaki
    • Journal Title

      Bioimages 11

      Pages: 61-66

    • NAID

      10013894043

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] 細胞生物学実験法III 細胞解析法(I)(分担執筆担当章名:エンドサイトーシス実験法(エンドソーム観察法))2004

    • Author(s)
      堺 立也
    • Publisher
      広川書店
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] 細胞生物学実験法III 細胞解析法(1)(分担執筆,担当章名:エンドサイトーシス実験法(エンドソーム観察法))2004

    • Author(s)
      堺 立也
    • Publisher
      広川書店
    • Related Report
      2004 Annual Research Report
  • [Publications] 堺立也: "宿主細胞表面でのインフルエンザウイルスのスライディング"日本臨床. 61・11. 1860-1863 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 水野敬文: "Difference-from-prediction imaging for high resolution shape analysis of locomoting cells."Bioimages. 11・2. 61-66 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 藤村宣憲: "The role of M cells of human nasopharyngeal lymphoid tissue in influenza virus sampling."Virchows Arch.. 444. 36-42 (2004)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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