| Project/Area Number |
15570141
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | Fukuoka University |
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Principal Investigator |
LEE Sannamu Fukuoka University, Faculty of Science, Research Associate, 理学部, 助手 (40248472)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAMI Noboru Fukuoka University, School of Medicine RI Center, Research Associate, 医学部・RI施設, 助手 (80154904)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2004: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2003: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | Nanotube / Amphuphilic peptide / Gold nanowire / Phospholipid / Lipisome / Neutron scattering in solution / Lipid helix / Structural biolog / 両親媒性ベプチド / 細胞内小胞輸送 / ペプチド / 両親媒性モデルペプチド |
|---|
| Research Abstract |
Cellular organelles, such as the Golgi apparatus and the endoplasmic reticulum, adopt characteristic structures depending on their function. While the tubular shapes of these structures result from complex protein-lipid interactions that are not fully understood, some fundamental machinery must be required. We show here that a de novo-designed 18-mer amphipathic a-helical peptide, Hel 13-5, transforms spherical liposomes made from a Golgi-specific phospholipid mixture into nanotubules on the scale of, and resembling the shape of, the nanotubules that form the Golgi apparatus. Furthermore, we show that that the size and the shape of such nanotubules depend on lipid composition, and peptide properties such as length, and the ratio of hydrophobic to hydrophilic amino acids. Although the question of precisely how nature engineers organellar membranes remains unknown, our simple system provides a basic set of tools to begin addressing this question.
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