| Project/Area Number |
15570144
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | Osaka University |
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Principal Investigator |
SHIMIZU Kikuo Osaka University, Radioisotope Research Center, Associate Professor, ラジオアイソトープ総合センター, 助教授 (20162696)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2004: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2003: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | yeast / DNA microarray / RNA / transcription / たんぱく発現 |
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| Research Abstract |
The new type mutant (degron mutant) was constructed in order to analyze the function of DNA polymerase φ in vivo. The degron mutant of POL5 (pol5^<td>) has the peptide which is substrate of proteolysis system of the ubiquitin in the upstream of the gene of DNA polymerase φ. This mutant also has plasmid for large manifesting UBC4 gene product of the degrading system, because it did not become the lethality in the high temperature (37 degrees) without overproducing the UBC4 gene product.
The DNA microarray analysis of the POL5 gene was carried out in order to examine the pattern of the gene expression at non-permissive temperature using the mutant pol5^<td> of DNA polymerase φ, and it was compared with the expression of the wild type by same condition. The result showed that the specific gene cluster peculiarly changed was not obtained.
This fact means that POL5 gene carries out universal transcriptional control not but specific gene group.
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