Novel DNA replication checkpoint in the assembly of DNA replication complex

• Project/Area Number: 15570155
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cell biology
• Research Institution: TOKYO INSTITUTE OF TECHNOLOGY
• Principal Investigator: TACHIBANA Kazunori TOKYO INSTITUTE OF TECHNOLOGY, Graduate School of Bio science and Biotechnology, Assistant Professor, 生命理工学研究科, 助手 (60212031)
• Project Period (FY): 2003 – 2004
• Project Status: Completed (Fiscal Year 2004)
• Budget Amount: ¥3,800,000 (Direct Cost: ¥3,800,000); Fiscal Year 2004: ¥1,100,000 (Direct Cost: ¥1,100,000); Fiscal Year 2003: ¥2,700,000 (Direct Cost: ¥2,700,000)
• Keywords: DNA REPLICATION / replication licensing / Mcm / Cdc45 / Checkpoint / Cell cycle / starfish / fertilization / MCM / Cdc45 / Cdc7 / Cdk2 / Cyclin E

Research Abstract

The regulation of the initiation of DNA replication in eukaryotic cells has been studied extensively the last enigmatic question is how cells form pre-initiation complex onto the DNA replication origins. The major factors involved in the assembly of the pre-initiation complex are Sld2-Dpb11,Sld3,GINS (Go, Ichi, Ni, and San), and S-phase kinases (Cdk;cyclin-dependent kinase, and Ddk,;Dbf4-dependent kinase). Recently, Wolter et al. reported that protein phosphatase 2A (PP2A) controls the pre-initiation complex in Xenopus cell-free system (J.Biol.Chem.2002). We have tested whether PP2A controlled the pre-initiation complex in starfish eggs. Neither the catalytic subunit of PP2A ligated with nuclear localization signal peptide sequence was ectopically expressed in starfish eggs nor okadaic acid, potent specific PP2A inhibitor was applied to starfish fertilized eggs, had significant effect to the initiation of the DNA replication.

Research Products

• [Journal Article] Distinct regulators for Plk1 activation in starfish meiotic and early embryonic cycles. (2003)
  • Author(s): Uchida-Okano, T., et al.
  • Journal Title: EMBO Journal 22 Pages: 5633-5642
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Distinct regulators for Plk1 activation in starfish meiotic and early embryonic cycles. (2003)
  • Author(s): Okano-Uchida T, Okumura E, Iwashita M, Yoshida H, Tachibana K, Kishimoto T.
  • Journal Title: EMBO J. 22 Pages: 5633-5642
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] PDK1 is required for the hormonal signaling pathway leading to meiotic resumption in starfish oocytes.
  • Author(s): Hiraoka, D, et al.
  • Journal Title: Developmental Biology (In press)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] PDK1 is required for the hormonal signaling pathway leading to meiotic resumption in starfish oocytes
  • Author(s): Hiraoka, D, Hori-Oshima, S, Fukuhara, T.Tachibana. K, Okumura, E, Kishimoto, T.
  • Journal Title: Devlop.Biol. (In press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Book] 発生における細胞増殖制御 (2004)
  • Author(s): 立花 和則(分担)
  • Total Pages: 223
  • Publisher: スプリンガーフェアラーク東京
  • Description: 「研究成果報告書概要(和文)」より
• [Book] 発生における細胞増殖制御 (2004)
  • Author(s): 竹内隆, 岸本健雄 監修(共著)
  • Publisher: スプリンガーフェアラーク東京
• [Publications] Okano, Uchida et al.: "Distinct regulators for PID1 activation in starfish meiotic and early embryonic cycles"The EMBO Journal. 22(20). 5633-5642 (2003)
• [Publications] 竹内隆, 岸本健雅編 立花和則(分担): "発生における細胞増殖制御"シュプリンガーフェアラーク東京. 198 (2004)