| Project/Area Number |
15570190
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Evolutionary biology
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| Research Institution | Fukuyama University |
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Principal Investigator |
HISATOMI Taisuke Fukuyama University, Faculty of Life Sciences and Biotechnology, Associate Prof., 生命科学部, 助教授 (20208763)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2004: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2003: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | Saccharomyces yeasts / speciation / sex-differentiation genes / evolution / reproductive isolation / molecular phylogeny / diversity of species / cell-cell interaction / 性的隔離 |
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| Research Abstract |
In order to clarify the mechanism of speciation in Saccharomyces yeasts, we attempted to compare sex-differentiation genes and chromosome organizations among several Saccharomyces yeasts. Research results we obtained are as follows.
1.We isolated a gene encoding an alpha mating pheromone from the yeast Saccharomyces naganishii. Then, we compared the structure of the gene with that of an orthologue from a laboratory strain of the yeast Saccharomyces cerevisiae. As a result, the coding regions of these two genes extremely resembled each other. However, the 5'-upstream regulatory regions from these two genes represented species-specific divergence.
2.We isolated a gene encoding a homothallism gene from a homothallic type strain of the yeast Saccharomyces paradoxus. Then, we compared the structure of the gene with that of an orthologue from a laboratory strain of the yeast Saccharomyces cerevisiae. As a result, it was shown that the coding regions and 5'-upstream regulatory regions from these two genes had coevolved through the evolutionary pathway.
3.We investigated the chromosomal localization of genes involved in galactose assimilation in the yeast Saccharomyces naganishii. It was revealed that the GAL10gene was translocated from the duster of GAL genes in the S.naganishii genome. It is very unique because the fact that the GAL10 genes are included in the GAL gene clusters has been reported so far.
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