| Project/Area Number |
15580002
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Breeding science
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| Research Institution | Hirosaki University |
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Principal Investigator |
HARADA Takeo Hirosaki University, Department of Agriculture and Life Science, Associate Professor, 農学生命科学部, 助教授 (10228645)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2004: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2003: ¥2,500,000 (Direct Cost: ¥2,500,000)
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| Keywords | Apple / Fruit Storage / Ripening / Firmness / Cultivar Fuji / DNA marker / Malus / Polygalacturonase / りんご / 細胞壁分解酵素 / DNAマーカー |
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| Research Abstract |
The characteristics of flesh softening during ripening process are greatly difference among apple cultivars. 'Fuji', leading cultivar in the world, doesn't show any detectable firmness-loss even under storage of room temperature at least one month, while 'Golden Delicious' starts the softening soon after harvesting. To elucidate the mechanism by which the distinction of softening pattern among cultivars accounts, four ripening related genes (MdACS1, MdACO1, MdPG1, MdExp3) were analyzed in 14 cultivars which represent the firmness-loss pattern of either 'Fuji' or 'Golden Delicious'. All the cultivars showing the character maintaining the firmness revealed weak or transient expression of MdPG1 irrespective of ethylene production rate and transcription levels of other genes. These results suggest that different softening behaviors during ripening among apple cultivars may be caused by distinctive expression patterns of MdPG1 gene.
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