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Studies on viroid inducing RNA silencing from the view of plant pathology and agricultural impact

Research Project

Project/Area Number 15580030
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant pathology
Research InstitutionHirosaki University

Principal Investigator

SANO Teruo  Hirosaki University, Fac.Agric & Life Sci., Professor, 農学生命科学部, 教授 (30142699)

Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2005: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2004: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2003: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsViroid / RNA silencing / Recovery / siRNA / Viroid homology sequence
Research Abstract

When tomato plants were infected with Potato spindle tuber viroid (PSTVd) at the cotyledon stage, primary leaf curling appeared on the 3rd leaf 14 days after inoculation. PSTVd reached the detectable all the leaves but for the meristem. PSTVd-specific siRNA also reached the detectable level in the 2nd-9th leaves. Accumulation of PSTVd and the siRNA increased to the highest level in the leaves showing severe leaf curling, however, PSTVd concentration decreased in the 9th and the upper leaves recovered from severe leaf curling. The results indicated that PSTVd-specific RNA silencing was triggered soon after PSTVd reached its detectable level, and PSTVd-specific siRNA spread quickly throughout the plant except for the meristem. The leaves being differentiated after induction of RNA silencing are to be recovered, since they are placed under the RNA silencing from the initial stage of the differentiation which resulted in the suppression of PSTVd accumulation.
Viroid-specific siRNAs of ca.21 and 23 nucleotides were detected in Hop stunt viroid-infected cucumber and hop, Hop latent viroid-infected hop and Apple fruit crinkle viroid-infected hop. On the other hand, only short siRNA of ca.21 nucleotides was detectable, in tomato infected with Cucumber mosaic virus.
Using in silica analysis by BLAST search in DNA data base, several tomato EST sequences of 18-20 nucleotides were identified. They were PCR amplified, cloned and sequenced. Using the cloned cDNAs as probes, the expression of the genes were analyzed, however, any positive signal was not identified.
Tomato seedlings obtained from healthy and viroid-infected fruits were equally infected with PSTVd challenge inoculation, which means that vertical transmission of viroid-induced RNA silencing was not apparent in tomato plants. On the other hand, the recovery was maintained in cuttings vegetatively propagated from PSTVd-infected tomato plants of the recovered stages.

Report

(4 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (29 results)

All 2006 2005 2004 2003 Other

All Journal Article (25 results) Book (2 results) Publications (2 results)

  • [Journal Article] First reports of two grapevine viroids in China and the possible detection of a third2006

    • Author(s)
      Shi-fang Li, Rui Guo, Tsuji, M., Sano, T.
    • Journal Title

      Plant Pathology (In Press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The first report of Coleus blumei viroid from coleus in China2006

    • Author(s)
      Shi-fang Li, Qian-fu Su, Rui Guo, Tsuji, M., Sano, T.
    • Journal Title

      Plant Pathology (In Press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Double-stranded RNA-specific ribonuclease confers tolerance against Chrysanthemum stunt viroid and Tomato spotted wilt virus in transgenic chrysanthemum plants.2005

    • Author(s)
      Ogawa, T., Toguri, T., Kudoh, H., Okamura, M., Monma, T., Yoshioka, M., Kato, K., Hagiwara, Y., Sano,T.
    • Journal Title

      Breeding Science 55

      Pages: 49-55

    • NAID

      10014472970

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Complete nucleotide sequences of attenuated and severe isolates of Leek yellow stripe virus from garlic in northern Japan : Identification of three distinct virus types in garlic and leek world-wide.2005

    • Author(s)
      Takaki, F., Sano, T., Yamashita, K., Fujita, T., Ueda, K., Kato,T.
    • Journal Title

      Arch. Virol. 150

      Pages: 1135-1149

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Occurrence of Chrysanthemum chlorotic mottle viroid in Japan2005

    • Author(s)
      Yamamoto, H., Sano, T.
    • Journal Title

      J. Gen. Plant Pathol. 71(2)

      Pages: 156-157

    • NAID

      10015507997

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Patterning of virus-infected soybean seed coat is associated with suppression of endogenous silencing of chalcone synthase genes2005

    • Author(s)
      Senda, M., Masuta, C., Ohnishi, K., Goto, K., Kasai, A., Sano, T., Hong, J.S., Macfarlane, S.
    • Journal Title

      Plant Cell 16

      Pages: 807-818

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Double-stranded RNA-specific ribonuclease confers tolerance against Chrysanthemum stunt viroid and Tomato spotted wilt virus in transgenic chrysanthemum plants.2005

    • Author(s)
      Ogawa, T., Toguri, T., Kudoh, H., Okamura, M., Monma, T., Yoshioka, M., Kato, K, Hagiwara, Y., Sano, T.
    • Journal Title

      Breeding Science 55

      Pages: 49-55

    • NAID

      10014472970

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Complete nucleotide sequences of attenuated and severe isolates of Leek yellow stripe virus from garlic in northern Japan : Identification of three distinct virus types in garlic and leek world-wide.2005

    • Author(s)
      Takaki, F., Sano, T., Yamashita, K., Fujita, T., Ueda, K., Kato, T.
    • Journal Title

      Arch.Virol. 150

      Pages: 1135-1149

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Occurrence of Chrysanthemum chlorotic mottle viroid in Japan2005

    • Author(s)
      Yamamoto, H., Sano, T.
    • Journal Title

      J.Gen.Plant Pathol. 71(2)

      Pages: 156-157

    • NAID

      10015507997

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Patterning of virus-infected soybean seed coat is associated with suppression of endogenous silencing of chalcone synthase genes.2005

    • Author(s)
      Senda, M., Masuta, C., Ohnishi, K., Goto, K, Kasai, A., Sano, T., Hong, J.S., Macfarlane, S.
    • Journal Title

      Plant Cell 16

      Pages: 807-818

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Double-stranded RNA-specific ribonuclease confers tolerance against Chrysanthemum stunt viroid and Tomato spotted wilt virus in transgenic chrysanthemum plants.2005

    • Author(s)
      Ogawa, T., Toguri, T., Kudoh, H., Okamura, M., Momma, T., Yoshioka, M., Kato, K., Hagiwara, Y., Sano, T.
    • Journal Title

      Breeding Science 55

      Pages: 49-55

    • NAID

      10014472970

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Characterization of a new viroid strain from hops : evidence for viroid speciation by isolation in different host specie2004

    • Author(s)
      Sano, T., Yoshida, H., Goshono, Monma, T., Kawasaki, H., Ishizaki, K.
    • Journal Title

      J. Gen. Plant Pathol. 70(3)

      Pages: 181-187

    • NAID

      10014195262

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Accumulation of short interfering RNAs characteristic of RNA silencing precedes recovery of tomato plants from severe symptom of potato spindle tuber viroid infection2004

    • Author(s)
      Sano, T., Matsuura, Y.
    • Journal Title

      J. Gen. Pl. Pathol. 70(1)

      Pages: 50-53

    • NAID

      10014194730

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Viroids-self-replicating non-coding RNAs (in Japanese)2004

    • Author(s)
      Sano, T.
    • Journal Title

      Kagaku to Seibutsu 42(8)

      Pages: 508-513

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Characterization of a new viroid strain from hops : evidence for viroid speciation by isolation in different host species2004

    • Author(s)
      Sano, T., Yoshida, H., Goshono, M., Monma, T., Kawasaki, H., Ishizaki, K.
    • Journal Title

      J.Gen.Pl.Pathol. 70(3)

      Pages: 181-187

    • NAID

      10014195262

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Accumulation of short interfering RNAs characteristic of RNA silencing precedes recovery of tomato plants from severe symptom of potato spindle tuber viroid infection2004

    • Author(s)
      Sano, T., Matsuura, Y.
    • Journal Title

      J.Gen.Pl.Pathol. 70(1)

      Pages: 50-53

    • NAID

      10014194730

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Characterization of a new viroid strain from hops : evidence for viroid speciation by isolation in different host species2004

    • Author(s)
      Sano, T., Yoshida, H., Goshono, M., Monna, 他2名
    • Journal Title

      J.Gen.Pl.Pathol. 70(3)

      Pages: 181-187

    • NAID

      10014195262

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Patterning of virus-infected soybean seed coat is associated with suppression of endogenous silencing of chalcone synthase genes.2004

    • Author(s)
      Send, M., Masuta, C., Ohnishi, K., Goto, K,.Kasai, A., Sano, T.他2名
    • Journal Title

      Plant Cell 16

      Pages: 807-818

    • Related Report
      2004 Annual Research Report
  • [Journal Article] ウイロイド-自律複製するノンコーディングRNA2004

    • Author(s)
      佐野 輝男
    • Journal Title

      化学と生物 42

      Pages: 508-513

    • NAID

      10016519386

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Identification of a novel structural interaction in Columnea latent viroid2003

    • Author(s)
      Owens, R.A., Sano, T., Feldstein, P.A., Hu, Y., Steger,G.
    • Journal Title

      Virology 313・(2)

      Pages: 604-614

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Identification of a novel structural interaction in Columnea latent viroid2003

    • Author(s)
      Owens, R.A., Sano, T., Feldstein, P.A., Hu, Y., Steger, G.
    • Journal Title

      Virology 313(2)

      Pages: 604-614

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] First reports of two grapevine viroids in China and the possible detection of a third

    • Author(s)
      Shi-fang Li, Rui Guo, Tsuji, M., Sano, T.
    • Journal Title

      Plant Pathology (Accepted for publication 08/Dec/15)(in Press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The first report of Coleus blumei viroid from coleus in China

    • Author(s)
      Shi-fang Li, Qian-fu Su, Rui Guo, Tsuji, M., Sano, T.
    • Journal Title

      Plant Pathology (Accepted for publication 10/Nov/05)(in Press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The first report of Coleus blumei viroid from coleus in China.

    • Author(s)
      Shi-fang Li, Qian-fu Su, Rui Guo, Tsuji, M., Sano T.
    • Journal Title

      Plant Pathology (In Press) (Accepted for publication 10/Nov/05)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] First reports of two grapevine viroids in China and the possible detection of a third

    • Author(s)
      Shi-fang Li, Rui Guo, Tsuji, M., Sano, T.
    • Journal Title

      Plant Pathology (In Press) (Accepted for publication 08/Dec/05)

    • Related Report
      2005 Annual Research Report
  • [Book] 植物病原アトラス2006

    • Author(s)
      佐野 輝男(分担執筆)
    • Total Pages
      270
    • Publisher
      ソフトサイエンス社
    • Related Report
      2005 Annual Research Report
  • [Book] 「研究成果報告書概要(和文)」より

    • Author(s)
      該当なし
    • Related Report
      2005 Final Research Report Summary
  • [Publications] Owens R.A.et al.: "Identification of a novel structural interaction in Columnea latent viroid"Virology. 313(2). 604-614 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Sano, T., Matsuura, Y: "Accumulation of short interfering RNAs characteristic of RNA silencing precedes recovery of tomato plants from severe symptom of potato spindle tuber viroid infection"J.Gen.Plant Pathol.. 70(1). 50-53 (2004)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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