Characterization and application of promoter activities associated with the multipartite ssDNA genome of plant Nanovirus.
| Project/Area Number |
15580032
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plant pathology
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| Research Institution | Niigata University |
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Principal Investigator |
SANO Yoshitaka Niigata University, Institute of Science and Technology, Assistant, 自然科学系, 助手 (00226044)
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| Project Period (FY) |
2003 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2005: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2004: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2003: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | Plant virus / Promoter |
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| Research Abstract |
Predicted promoter regions of Milk vetch dwarf virus (MIDV) components (C1-C11) were isolated and fused with a β-glucuronidase (GUS) reporter gene and the characteristics of the promoters were examined. In transgenic tobacco calli, promoters of MDV C4,C5 and C7,C6 and C8 generated a stronger level of GUS expression than the Cauliflower mosaic virus 35S RNA promoter (P35S). In leaves of transgenic tobacco plants, the promoters of C5 and C8 conferred a level of GUS activity comparable to that of P35S. Histochemical GUS analysis showed that the promoters of C4-C9, the latter encoding a capsid protein, were active in phloem and meristematic tissue. The promoter of C8 was also active in mesophyll and cortex cell types. The usefulness of the MDV-C8 promoter (PMC8) was further confirmed by driving NPTII for selection of kanamycin-resistant tobacco plants with improved transformation efficiency. PMC8 was also effective in transgenic rice plants. Thus, PMC8 is useful as an alternative to P35S in both dicotyledonous and monocotyledonous plants, especially for gene expression in proliferating tissues. During the period of present research, the host range of MDV was further examined, and it was revealed that, besides the known legume plant hosts, MDV systemically infect several non-legume plants including Spinacia oleracea, Stellaria media and Arabidopsis thaliana. These facts have implications for the etiology of MDV, for which the summer hosts have not known.
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Report
(4 results)
Research Products
(13 results)
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[Journal Article] The promoter of Milk vetch dwarf virus component 8 confers effective gene expression in both dicot and monocot plants2005
Author(s)
N.Shirasawa-Seo, Y.Sano, S.Nakamura, T.Murakami, Y.Gotoh, Y.Naito, C.N.Hsia, S.Seo, I.Mitsuhara, S.Kosugi, Y.Ohashi
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Journal Title
Plant Cell Reports 24
Pages: 155-163
Description
「研究成果報告書概要(和文)」より
Related Report
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[Journal Article] The promoter of Milk vetch dwarf virus component 8 confers effective gene expression in both dicot and monocot plants.2005
Author(s)
N.Shirasawa-Seo, Y Sano, S.Nakamura, T.Murakami, Y.Gotoh, Y.Naito, C.N.Hsia, S.Seo, I.Mitsuhara, S.Kosugi, Y.Ohashi
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Journal Title
Plant Cell Reports 24
Pages: 155-163
Description
「研究成果報告書概要(欧文)」より
Related Report
-
[Journal Article] The promoter of Milk vetch dwarf virus component 8 confers effective gene expression in both dicot and monocot plants.2005
Author(s)
N.Shirasawa-Seo, Y.Sano, S.Nakamura, T.Murakami, Y.Gotoh, Y.Naito, C.N.Hsia, S.Seo, I.Mitsuhara, S.Kosugi, Y.Ohashi
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Journal Title
Plant Cell Reports 24
Pages: 155-163
Related Report
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[Book] Nanoviridae, Virus Taxonomy, VIIIth Report of the ICTV2004
Author(s)
Vetten, H.J., Chu, P.W.G., Dale, J.L., Harding, R., Hu, J., Katul, L., Kojima, M., Randles, J.W., Sano, Y., Thomas, J.E
Total Pages
1259
Publisher
Elsevier/Academic Press, London
Description
「研究成果報告書概要(和文)」より
Related Report
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