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Molecular evaluation of volatiles in plant defense

Research Project

Project/Area Number 15580035
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant pathology
Research InstitutionKagawa University

Principal Investigator

AKIMITSU Kazuya  Kagawa University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (80263888)

Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2004: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2003: ¥1,700,000 (Direct Cost: ¥1,700,000)
Keywordscitrus / monoterpene / plant / non-mevalonic acid pathway / signal transduction / Alternaria / ラフレモン / 抵抗性 / 揮発性物質 / 傷処理 / 菌接種
Research Abstract

Various types of volatiles have been known to produce and release from plant cells. We have studied roles and importance of several different volatiles including monoterpenes, which are the major component of citrus volatiles. Eleven monoterpenes were tested their biological functions, such as fungicidic acitivity, anti-insect activity, and signal transduction activity. Mevalonic acid and non-mevalonic acid pathways have been Known to be involved for biosynthesis of terpenoids and C10 monoterpenes would be synthesized by GPP from non-mevalonic acid pathway in chloroplast through monoterpene pathway. There are at least seven enzymes involved in non-mevalonic acid pathway, and a citrus gene encoding one of these enzymes, 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF), was cloned. A localization analysis using GFP as the reporter indicated that the gene product is located in chloroplast.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (19 results)

All 2004 2003 Other

All Journal Article (9 results) Book (2 results) Publications (8 results)

  • [Journal Article] Dissection of the host range of the fungal plant pathogen Alternaria alternata by modification of the secondary metabolism.2004

    • Author(s)
      Ito, K., Tanaka, T., Hatta, R., Yamamoto, M., Akimitsu, K., Tsuge, T.
    • Journal Title

      Molecular Microbiology 52

      Pages: 399-411

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Molecular, ecological and evolutionary approaches to understanding Alternaria diseases of citrus.2003

    • Author(s)
      Akimitsu, K., Peever, T.L., Timmer, L.W.
    • Journal Title

      Molecular Plant Pathology 4

      Pages: 435-446

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Epoxide hydrolase : A mRNA induced by a fungal pathogen Alternaria alternata on rough lemon(Citrus jambhiri Lush).2003

    • Author(s)
      Gomi, K., Yamasaki, Y., Yamamoto, H., Akimitsu, K.
    • Journal Title

      Plant Molecular Biology 53

      Pages: 189-199

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Characterization of a hydroperoxide lyase gene and effect of C6-volatiles on expression of genes of the oxylipin metabolism in Citrus.2003

    • Author(s)
      Gomi, K., Yamasaki, Y., Yamamoto, H., Akimitsu, K.
    • Journal Title

      Journal of Plant Physiology 160

      Pages: 1219-1231

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Alternaria diseases of citrus-Novel pathosystems.2003

    • Author(s)
      Timmer, L.W., Peever, T.L., Dolel, Z., Akimitsu, K.
    • Journal Title

      Phytopathologia Mediterranea 42

      Pages: 99-112

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Green fluorescent detection of fungal colonization and endopolygalacturonase gene expression in the interaction of Alternaria citri with citrus.2003

    • Author(s)
      Isshiki, A., Ohtani, K., Kyo, M., Yamamoto, H., Akimitsu, K
    • Journal Title

      Phytopathology 93

      Pages: 768-773

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Epoxide hydrolase : A mRNA induced by a fungal pathogen Alternaria alternata on rough lemon (Citrus jambhiri Lush).2003

    • Author(s)
      Gomi, K., Yamasaki, Y., Yamamoto, H., Akimitsu, K.
    • Journal Title

      Plant Molecular Biology 53

      Pages: 189-199

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Alternaria diseases of citrus - Novel pathosystems.2003

    • Author(s)
      Timmer, L.W., Peever, T.L., Dolel, Z., Akimitsu, K.
    • Journal Title

      Phytopathologia Mediterranea 42

      Pages: 99-112

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Alternaria diseases of citrus - Novel pathosystems.2003

    • Author(s)
      Timmer, L.W., Peever, T.L, Dolel, Z., Akimitsu, K.
    • Journal Title

      Phytopathologia Mediterranea 42

      Pages: 99-112

    • Related Report
      2004 Annual Research Report
  • [Book] Biology of Molecular Plant-Microbe Interactions, Volume 42004

    • Author(s)
      Ohtani, K., Masunaka, A., Yamasaki, Y., Yamamoto, H., Akimitsu, K.
    • Total Pages
      632
    • Publisher
      APS Press
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Book] 植物病の探求2004

    • Author(s)
      秋光 和也, 山本 弘幸
    • Total Pages
      188
    • Publisher
      三重大学
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Publications] Gomi, K., Yamamoto, H., Akimitsu, K.: "Epoxide hydrolase : A mRNA induced by a fungal pathogen Alternaria alternata on rough lemon (Citrus jambhiri Lush)."Plant Molecular Biology. 53. 189-199 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Gomi, K., Yamasaki, Y., Yamamoto, H., Akimitsu, K.: "Characterization of a hydroperoxide lyase gene and effect of C6-volatiles on expression of genes of the oxylipin metabolism in Citrus."Journal of Plant Physiology. 160. 1219-1231 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Akimitsu, K., Peever, T.L., Timmer, L.W.: "Molecular, ecological and evolutionary approaches to understanding Alternaria diseases of citrus."Molecular Plant Pathology. 4. 435-446 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Timmer, L.W., Peever, T.L., Dolel, Z., Akimitsu, K.: "Alternaria diseases of citrus - Novel pathosystems."Phytopathologia Mediterranea. 42. 99-112 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 山崎 祐未子, 田村 啓敏, 久能 均, 山本 弘幸, 秋光 和也: "揮発性モノテルペンのAlternaria alternataの胞子発芽および菌糸伸長への作用"日本植物病理学会報. 69. 53 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 山崎祐未子, 田村 啓敏, 山本弘幸, 秋光和也: "モノテルペン合成に関与する酵素遺伝子の部分領域の増幅"日本植物病理学会報. 69. 244 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 山崎 祐未子, 田村 啓敏, 久能 均, 山本 弘幸, 秋光 和也: "Alternaria alternataを噴霧接種したラフレモン葉からのモノテルペン放出様式"日本植物病理学会講演要旨集. 12 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 山崎祐未子, 久能 均, 山本弘幸, 秋光和也: "カンキツ病害抵抗性誘導における揮発性モノテルペンの役割について"日本植物病理学会講演要旨集. 164 (2004)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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