| Project/Area Number |
15580048
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plant nutrition/Soil science
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| Research Institution | Hokkaido University (2004)
Nagoya University (2003) |
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Principal Investigator |
EZAWA Tatsuhiro Hokkaido Univ., Grad.Sch.Agri., Assoc.prof., 大学院・農学研究科, 助教授 (40273213)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2004: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2003: ¥2,500,000 (Direct Cost: ¥2,500,000)
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| Keywords | arbuscular mycorrhizal fungi / symbiosis / phosphate / polyphosphate / vacuole / assessment / acidic soil / acid tolerant |
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| Research Abstract |
I.Analysis of the rate of polyphosphate translocation through hyphae
We have established a method to quantify polyphosphate (polyP) in hyphae of arbuscular mycorrhizal fungi using the polyphosphate kinase/luciferase system. The rate of polyP translocation was compared between Glomus sp.HR1, Archaeospora leptoticha OK-15 and Glomus etunicatum in symbiosis with Lotus japonicus under acidic (pH 4.3) and neutral (pH 7.0) conditions. Glomus sp. HR1 and A.leptoticha OK-15 which were isolated from acidic soil promoted the growth of the host at pH 4.3 and 7.0. In contrast, G.etunicatum, an isolate from neutral soil, colonized and promoted the growth of the host only at pH 7.0 but not colonized at pH 4.3. The rate of polyP translocation in Glomus sp. HR1 and A.leptoticha OK-15 was significantly different between the isolates, 1.11 and 0.48 min h^<-1>, respectively, the influence of soil pH on the rate was not observed.
II.Ecology of arbuscular mycorrhizal fungi in acid sulfate soil
We succeeded to isolate arbuscular mycorrhizal fungi from the rhizosphere of pioneer plants in acid sulfate soils in Hazu-cho, Aichi and Rankoshi-cho, Hokkaido. It was found that Glomus sp. HR1 from Aichi and Glomus sp. RF1 from Hokkaido belonged to the same cluster based on a phylogenetic analysis of 18S rRNA gene, this suggested that these fungi adapted to acidic environment. The involvement of fungal diversity in the growth of pioneer plants was investigated by a pot experiment using rhizosphere soil collected from several pioneer plants. The growth of the plants was greater in the soil showing higher fungal diversity, indicating the significance of fungal diversity in the survival and growth of the pioneer plants.
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