| Project/Area Number |
15580055
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Tohoku University |
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Principal Investigator |
KANEKO Jun Tohoku University, Graduate School of Agricultural Science, Research Associate, 大学院・農学研究科, 助手 (30221188)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2004: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2003: ¥2,700,000 (Direct Cost: ¥2,700,000)
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| Keywords | Staphylococcus aureis / Panton-Valentine leukocidin / phage conversion / epidemiology / host recognition / Staphlococcus aureis |
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| Research Abstract |
Staphylococcal Panton-Valentine leukocidin (PVL) is an important virulence factor, which causes leukocytolysis and tissue necrosis. PVL genes are highly associate with community-acquired methicillin-resistant S.aureus (CA-MRSA), which is appear to be worldwide emerging pathogen. We suggested that horizontal transmission of PVL genes have been occured by at least two types of PVL-carrying temperate bacteriophage among S.aureus. In this study, we determine the genome of several PVL-carrying phages and found the polymorphysm of PVL-carrying phage genomes. We also found the polymorphism of the orf containing attB core sequence for φPVL/φSLT. By using these results, we construct the PCR scan system for PVL-carrying phage typing which will become useful tool for the epidemiological study of PVL carrying S aureus strain including CA-MRSA. Coagulase typing of PVL-carrying strains was also performed and partial relationship between PVL-phage and coagulase types were observed.
In addition, a novel structural protein ORF636 with 66 kDa of φSLT, which contains two 97-amino acid sequence repeats with the sequence GW in its C-terminal region, was identified as an essential structural protein for the adhesion of φSLT to S.aureus through staphylococcal lipoteichoic acid.
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