| Project/Area Number |
15580057
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Saitama university |
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Principal Investigator |
MATSUZAKI Hiroshi Saitama university, Molecular Analysis and Life Science Center, 総合科学分析支援センター, 助教授 (80008870)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2004: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2003: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | Phospholipids / Escherichia coli / GTP binding protein / G protein / Mutants / Membrane phospholipids deficient mutation / Cellular localization / tranccription and translation / Eastern blotting / 膜リン脂質合成欠損変異 / トランスポゾン挿入失活変異 |
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| Research Abstract |
This studies aim the elucidation of the function of GTP binding protein (G protein) to be concerned with the function of the membrane phospholipids in E.coli. The influence on the transcription or translation activities in the membrane phospholipids deficient mutation etc. stock was examined by using a promoter strength measurement system that made strong fluorescence GFP as a reporter gene and a real-time RT-PCR method about low molecular weight G protein gene yihA, era, and obgE essential on growth in E..coli. A clear difference was not admitted in the cardiolipin deficient mutation and the transcription activity of low molecular weight G protein gene increased remarkably in the acidic phospholipids deficient mutation (pgsA3 mutation stock) compared with the wild-type strain. Thus, it was suggested that the membrane phospholipid composition, especially the increase and decrease of the acidic pospholipid content take part in the appearance control.
【summary of research and view】G protein gene, yihA gene of an essential G protein of E.coli were admitted the increase of the transcript revitalization in the acid phosphorus lipid synthesis loss mutation stock, and clarified that this expression depended on the expression of the acidic phospholipid synthesis enzyme gene. Der (or Yfg) related to presumption G protein localized in the septum in a cell by binding with GFP in the complete amphiphilic phospholipid defincient mutation. The above-mentioned result depends on the amount of the acidic phospholipid by the expression of G protein and suggests G protein, the membrane lipid, and the possibility of the interaction with the biomembrane strongly.
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