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Signaling mechanism of fruit body formation of basidiomycetous mushrooms

Research Project

Project/Area Number 15580073
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied biochemistry
Research InstitutionShinshu University

Principal Investigator

SEN Kikuo  Shinshu University, Faculty of Agriculture, Professor (80196980)

Co-Investigator(Kenkyū-buntansha) 柴井 博四郎  信州大学, 農学部, 教授 (60313839)
Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2005: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2004: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2003: ¥1,900,000 (Direct Cost: ¥1,900,000)
Keywordsbasidiomycetes / mushrooms / Schizophyllum commune / fruit body formation / signal transduction / tryptophan / indole / caffeine / イドール
Research Abstract

This study aims to elucidate the signaling mechanism of sexual development of basidiomycetous mushrooms from mating to fruit body formation. Here, stimulation factors for sexual development of the model basidiomycete, Schizophyllum commune, were researched from both viewpoints of environmental factors (external signal factors) and genetic factors.
1. Environmental factors (external signal factors)
Effects of external signal factors on fruit body formation of S.commune were examined by addition of indole-related compounds to agar media. At the low concentration of 100μg/ml, effects on fruit body formation of indole (Ind), 3-indoleacetic acid (IAA), 3-indolecarboxylic acid (ICA), 3-indolepropionic acid (IPA) and tryptophan (Trp) were as follows : IBA>ICA≧IPA≧IAA>Ind≧Trp. This result showed that compounds containing a structural moiety of indole stimulated fruit body formation.
2. Genetic factors
Trp-systhesizing gene of S.commune, TRP1, suppresses abnormality in sexual development (abnormal mating and nuclear migration) induced by indole-resistant mutation (ind1) and caffeine-resistant mutation (cfn1). Sequencing analysis of the TRP1 showed that its product was an enzyme with three catalytic regions. One of the three regions, IGPS (indole-3-glycerol phosphate synthetase) had ability to suppress the abnormal sexual development. Therefore, highly expressed IGPS gene was constructed, and dikaryon with high expression of IGPS was made using the IGPS gene constructed. The dikaryon with high expression of IGPS formed fruit bodies 10 days earlier than dikaryons with no or usual expression of IGPS externally introduced. This result showed that the IGPS region in TRP1 had ability to stimulate sexual development of wild-typed strains as well as the ability to suppress abnormal sexual development of ind1 and cfn1 mutant strains.

Report

(4 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (4 results)

All 2004 2003

All Presentation (4 results)

  • [Presentation] スエヒロタケTRP1遺伝子のIGPS領域はindl変異による性生長異常を抑制する2004

    • Author(s)
      瀬川保宏, 他
    • Organizer
      日本菌学会第48回大会
    • Place of Presentation
      長崎
    • Year and Date
      2004-05-30
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Presentation] The IGPS region of TRP1 gene of Schizophyllum commune suppresses abnormal sexual development by the ind1 mutation2004

    • Author(s)
      SEGAWA, Y., et al.
    • Organizer
      The 48th Annual Meeting of the Mycological Society of Japan
    • Place of Presentation
      Nagasaki
    • Year and Date
      2004-05-30
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Presentation] スエヒロタケ(Schizophyllum commune)のTRP1遺伝子の性生長調節領域2003

    • Author(s)
      千菊夫, 他
    • Organizer
      日本菌学会第47回大会
    • Place of Presentation
      札幌
    • Year and Date
      2003-05-31
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Presentation] A sexual development-regulating region of TRP1 gene of Schizophyllum commune2003

    • Author(s)
      SEN, K., et al.
    • Organizer
      The 47th Annual Meeting of the Mycological Society of Japan
    • Place of Presentation
      Sapporo
    • Year and Date
      2003-05-31
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary

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Published: 2003-04-01   Modified: 2016-04-21  

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