| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 2004: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2003: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Research Abstract |
Upon the completion of the Human Genome Project, huge numbers of genetic sequences are now available. However, the function of many of these genes has not been identified. Therefore, the identification of the function of these genes is one of the most important purposes of the study of Proteomics.
Now, it is well known that many of the posttranslational modifications such as proteolytic processing, phosphorylation, glycosylation, lipid modifications, ADP-ribosylation etc. play critical roles in the structure and function of protein and regulate many cellular events such as intracellular signal transduction. However, systematic method to analyze the posttranslational modification of protein has not been established.
In the present study, we have shown that targeting, processing and modification of a distinct protein could be successfully detected by using metabolic labeling in an in vitro translation system or in transfected cells. Since many of these posttranslational modifications play specific roles in the structure and function of protein, it might be possible to predict the function of the protein from these experimental observations.
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