• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Enzymatic stabilization and functionarization of polyphenol glucosides such as plant pigments for further utilization of their bio-functions.

Research Project

Project/Area Number 15580083
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied biochemistry
Research InstitutionOkayama Prefectural University

Principal Investigator

NAKAJIMA Nobuyoshi  Okayama Prefectural University, Department of Nutritional Science, Ph.D., Associate Professor, 保健福祉学部, 助教授 (10198070)

Co-Investigator(Kenkyū-buntansha) ISHIHARA Kohji  Okayama University of Science, Department of Life Science, Ph.D., Assistant Professor, 理学部, 講師 (50273537)
Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2005: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2004: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2003: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsPlant Pigment / Anti-oxidant / Glucosylation / Acylation / Lipase / UDP-glucosyltransferase / Food ingredient / Biofunction / 酵素酵素 / 天然植物色素 / アントシアニン / アシル化色素 / 芳香族酸
Research Abstract

We have investigated a new enzymatic method for glucosylation of naturally-occurring-(poly) phenol compounds by using of UDP-glucoslytransferase reaction. We have also developed an enzymatic procedure for acylation of the (poly) phenol glucosides such as plant pigments by using of lipase transesterification reaction with vinyl aromatic acids as an acyl-donor. As the results, it was possible for the stabilization and functionarization of the naturally-occurring-(poly) phenolic compounds in order to further utilization of their bio-functions.

Report

(4 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (31 results)

All 2006 2005 2004 2003 Other

All Journal Article (24 results) Book (3 results) Publications (4 results)

  • [Journal Article] Molecular and Biochemical Characterization of Serine Racemase from Arabidopsis thaliana2006

    • Author(s)
      M.Sugimoto, N.Nakajima, et al.
    • Journal Title

      Phytochemistry (in press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Biotransformation of Capsaicinoids by Immobilized Plant Cultured Cells.2006

    • Author(s)
      K.Ishihara, N.Nakajima, et al.
    • Journal Title

      J. Biosci. Bioengin., (in press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] An Isozyme of Earthworm Serine Protease….2005

    • Author(s)
      N.Nakajima, et al.
    • Journal Title

      Biosci.Biotechnol.Biochem. 69(10)

      Pages: 2009-2011

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Analysis of Isoflavone Content in Temphe, ….2005

    • Author(s)
      N.nakajima, et al.
    • Journal Title

      J.Biotechnol.Bioengin. 100(6)

      Pages: 685-687

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Analysis of Isoflavone Content in Tempeh, a Fermented Soybean, and Preparation of a New Isoflavone-Enriched Tempeh.2005

    • Author(s)
      N.Nakajima, N.Nozaki, K.Ishihara, A.Ishikawa, H.Tsuji.
    • Journal Title

      J.Biosci.Bioeng. 100(6)

      Pages: 685-687

    • NAID

      110002978303

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] An Isozyme of Earthworm Serine Protese Acts on Hydrolysis of Triacylglyceral.2005

    • Author(s)
      N.Nakajima, et al.
    • Journal Title

      Biosci. Biotechnol. Biochem., 69(10)

      Pages: 2009-2011

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Anlysis of Isoflavone Content in Tempeh, a Fermented Soybean, and Preparation of a New Isoflavone-Enriched Tempeh.2005

    • Author(s)
      N.Nakajima, et al.
    • Journal Title

      J. Biosci. Bioengin., 100(6)

      Pages: 685-687

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Inhibitory Effect of Glycolipids from Spinach on in vitro and ex vivo angiogenesis.2005

    • Author(s)
      K.Matsubara, N.Nakajima, et al.
    • Journal Title

      Oncology Rep., 14

      Pages: 157-160

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 感染症の症状を軽減した耐病性日本産アコヤガイ系統の開発2005

    • Author(s)
      内村裕之, 中島伸佳, 他
    • Journal Title

      水産育種 34

      Pages: 91-97

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Anti-angiogenic Activity of Quercetin….2004

    • Author(s)
      K.Matsubara, N.Nkajima, et al.
    • Journal Title

      Lett.Drug.Desig.Discov. 1

      Pages: 329-333

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Biotransformation of (+)-Catechin by Plant….2004

    • Author(s)
      K.Ishihara, N.Nakajima, H.Hamada, et al.
    • Journal Title

      Plant Biotechnol. 21(5)

      Pages: 407-409

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Anti-angiogenic Activity of Quercetin and its Derivatives.2004

    • Author(s)
      K.Matsubara, K.Ishihara, Y.Mizushina, M.Mori, N.Nakajima
    • Journal Title

      Lett. Drug Desi. and Disc. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Biotransformation of (+)-Catechin by Plant Cultured Cells of Eucalyptus periniana.2004

    • Author(s)
      S.Otani, Y.Kondo, Y.Asada, T.Furuya, H.Hamada, N.Nakajima, K.Ishihara, H.Hamada
    • Journal Title

      Plant Biotechnol. 21(5)

      Pages: 407-409

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Enzymatic oxidation of 1,4- and 1,8-cineole using plant cultured cells of Catharanthus Roseus2004

    • Author(s)
      Hiroki Hamada, Nobuyoshi Nakajima, et al.
    • Journal Title

      Lett.Org.Chem. 1

      Pages: 171-172

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Anti-angiogenic activity of quercetin and its derivatives.2004

    • Author(s)
      K.Matsubara, Nobuyoshi Nakajima, et al.
    • Journal Title

      Lett.Drug.Desig.Descov. 1

      Pages: 329-333

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Conversion of cofactor specificity of alanine dehydrogenases by site-directed mutagenesis2004

    • Author(s)
      Hiroyuki Ashida, Nobuyoshi Nakajima, et al.
    • Journal Title

      J.Mol.Catal.B : Enzym. 30

      Pages: 173-176

    • Related Report
      2004 Annual Research Report
  • [Journal Article] A novel zinc-containing α-keto ester reductase from Actinomyces : an approach based on protein chemistry ・・・2004

    • Author(s)
      Kohji Ishihara, Nobuyoshi Nakajima, et al.
    • Journal Title

      Biosci.Biotechnol.Biochem. 68(10)

      Pages: 2120-2127

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Purification and Characterization of α-Keto amide reductase from Saccharomyces cerevisiae.2004

    • Author(s)
      Kohji Ishihara, Nobuyoshi Nakajima, et al.
    • Journal Title

      Biosci.Biotechnol.Biochem. 68(11)

      Pages: 2306-2312

    • NAID

      130000030919

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Biotransformation of (+)-catechin by plant cultured cells of Eucalyptus perriniana.2004

    • Author(s)
      Shuichi Otani, Nobuyoshi Nakajima, et al.
    • Journal Title

      Plant Biotechnol. 21(5)

      Pages: 407-409

    • NAID

      130000064280

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Structural Aspects of Acylated Plant Pigment….2003

    • Author(s)
      K.Ishihara, N.Nakajima
    • Journal Title

      J.Mol.Catal.B : Enzymn. 23

      Pages: 411-417

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Comporison of Acylated Plant Pigments….2003

    • Author(s)
      N.Nakajima, et al.
    • Journal Title

      Biosci.Biotechnol.Biochem. 67(8)

      Pages: 1828-1831

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Comparison on Acylated Plant Pigments : Light-resistibility and Radical Scavenging Ability.2003

    • Author(s)
      N.Nakajima, M.Sugimoto, H.Yokoi, H.Tsuji, K.Ishihara
    • Journal Title

      Biosci.Biotech.Biochem. 67(8)

      Pages: 1823-1832

    • NAID

      110002694311

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Biotransformation using Plant Cultured Cells.2003

    • Author(s)
      K.Ishihara, H.Hamada, T.Hirata, N.Nakajima
    • Journal Title

      J.Mol.Catal.B : Enzymatic 23

      Pages: 145-170

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Structural Aspects of Acylated Plant Pigments : Stabilization of Flavonoid Glucosides and Interpretation of their Functions.2003

    • Author(s)
      K.Ishihara, N.Nakajima
    • Journal Title

      J.Mol.Catal.B : Enzymatic 23

      Pages: 411-417

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] Bioindustry:特集,物質合成の設計図にブレークスルーを,酵素触媒の活躍と未来2006

    • Author(s)
      中島伸佳
    • Total Pages
      78
    • Publisher
      シーエムシー出版(株)
    • Related Report
      2005 Annual Research Report
  • [Book] Handbook of Proteolytic Enzyme 2^<nd> Edn.2004

    • Author(s)
      A.J.Barret.et al.
    • Total Pages
      2140
    • Publisher
      Elsevier Ltd
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] Handbook of Proteolytic Enzyme, 2^<nd>, Edn.2004

    • Author(s)
      A.J.Barret, et al.
    • Total Pages
      2140
    • Publisher
      Elsevier Ltd(ISBN-0-12-079610-4)
    • Related Report
      2004 Annual Research Report
  • [Publications] K.Ishihara, N.Nakajima, et al.: "Biotransformation using plant cultured cells."J.Mol.Catal. B : Enzym.. 23・2-6. 145-170 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] K.Ishihara, N.Nakajima: "Structural aspects of acylated plant pigments : Stabilization of flavonoid glucosides and interpretation of their functions."J.Mol.Catal. B : Enzym.. 23・2-6. 411-417 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] N.Nakajima, K.Ishihara, et al.: "Comparison of acylated plant pigments : light-resistance and radical-scavenging ability."Biosci.Biotechol.Biochem.. 67・8. 1828-1831 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] H.Hamada, K.Ishihara, N.Nakajima, et al.: "One-step glucosylation of capsaicinoids by cultured cells."Plant Biotechnol.. 20・3. 253-255 (2003)

    • Related Report
      2003 Annual Research Report

URL: 

Published: 2003-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi