Identification of causative gene for type 2 diabetes in SMXA-5 mouse feeding a high fat diet.
| Project/Area Number |
15580105
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Food science
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| Research Institution | Chubu University (2004)
Nagoya University (2003) |
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Principal Investigator |
HORIO Fumihiko Chubu University, College of Bioscience & Biotechnology, Professor, 応用生物学部, 教授 (20165591)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIMURA Masahiko Nagoya University, Graduate School of Medicine, Professor, 大学院・医学系研究科, 教授 (20073661)
IKEGAMI Hiroshi Osaka University, Graduate School of Medicine, Associate Professor, 大学院・医学系研究科, 助教授 (20221062)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2004: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2003: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | Type 2 diabetes / Disease gene / QTL analysis / High-fat diet / Nutritional Biochemistry / Genetics / 糖尿病モデルマウス / 糖尿病遺伝子 |
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| Research Abstract |
The SMXA-5 mouse is one of the 26 SMXA recombinant inbred (RI) substrains that have been established from nondiabetic SMIJ and A/J strains. This mouse is a model for polygenic type 2 diabetes characterized by both moderate impairment of glucose tolerance and mild hyperinsulinemia. To dissect A/J-derived diabetogenic loci of SMXA-5 contributing to diabetes-related traits such as impaired glucose tolerance, hyperglycemia, hyperinsulinemia, and obesity, we attempted to analyze the quantitative trait loci (QTL) in (SM/J x SAM-5)F2 intercross mice fed a high-fat diet. A major QTL for body mass index, nonfasting blood glucose concentration, and glucose tolerance was mapped on Chr 2. This locus existed near D2Mit15, with the highest logarithm of odds (LOD) score, 12.5, for glucose concentration being recorded at 120 min in an intraperitoneal glucose tolerance test (IPGTT). We designated this QTL as t2dm2sa, for type 2 diabetes mellitus 2 in the SMXA RI strains. SM.A-t2dm2sa, a congenic strain that introgressed the A/J-derived t2dm2sa region into SM/J, exhibited overt impaired glucose tolerance and mild hyperinsulinemia under a high-fat diet. These results suggested that latent diabetogenic genes exist in the genomes of nondiabetic AM and SM/J mice, and that interaction between t2dm2sa and an unknown SM/J-derived locus except where t2dm2sa exists, elicits impaired glucose tolerance in SMXA-5 and SM.A-t2dm2sa mice. The dissection of these diabetogenic genes that have epistatic effects will contribute to the elucidation of the complex mechanisms underlying human type 2 diabetes.
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Report
(3 results)
Research Products
(26 results)
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[Journal Article] SMXA-5 mouse as a diabetic model susceptible to feeding a high-fat diet.2004
Author(s)
Kobayashi, M., Io, F., Kawai, T., Nishimura, M., Ohno, T., Horio, F
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Journal Title
Biosci.Biotechnol.Biochem. 68
Pages: 226-230
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Anthocyanin enhances adiopocytokine secretion and adipocyte-specific gene expression in isolated rat adipocytes.2004
Author(s)
Tsuda, T., Ueno, Y., Aoki, H., Koda, T., Horio, F., Takahashi, N., Kawada, T., Osawa, T.
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Journal Title
Biochem.Biophys.Res.Commun. 316
Pages: 149-157
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Suppressive effect of hot water extract od Adzuki beans (Vigna angularis) on hyperglycemia after sucrose loading in mice and diabetic rats.2004
Author(s)
Itoh, T., Kita, N., Kurokawa, Y., Kobayashi, M., Horio, F.Furuichi, Y.
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Journal Title
Biosci.Biotechnol.Biochem. 68
Pages: 2421-2426
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] SMXA-5 mouse as a diabetic model susceptible to feeding a high-fat diet.2004
Author(s)
Kobayashi, M., Io, F., Kawai, T., Nishimura, M., Ohno, T., Horio, F
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Journal Title
Biosci.Biotechnol. Biochem. 68(1)
Pages: 226-230
Related Report
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[Journal Article] Anthocyanin enhances adiopocytokine secretion and adipocyte-specific gene expression in isolated rat adipocytes.2004
Author(s)
Tsuda, T., Ueno, Y., Aoki, H., Koda, T., Horio, F., Takahashi, N., Osawa, T.
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Journal Title
Biochem.Biophys.Res.Commun. 316(6)
Pages: 149-157
Related Report
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[Journal Article] Quantitative trait locus analysis of serum insulin, triglyceride, total cholesterol and phospholipid levels in the (SM/JxA/J)F2 mice.2003
Author(s)
Anunciado, R.V.P., Nishimura, M., Mori, M., Ishikawa, A., Tanaka, S., Horio, F., Ohno, T., Namikawa, T.
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Journal Title
Exp.Anim. 52
Pages: 37-42
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Changes in catecholamine metabolism by ascorbic acid deficiency in spontaneously hypertensive rats unable to synthesize ascorbic acid.2003
Author(s)
Kawai, K., Ito, H., Kubota, H., Takemori, K., Makino, S., Horio, F.
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Journal Title
Life Sci. 72
Pages: 1717-1732
Description
「研究成果報告書概要(欧文)」より
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