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Identification of causative gene for type 2 diabetes in SMXA-5 mouse feeding a high fat diet.

Research Project

Project/Area Number 15580105
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Food science
Research InstitutionChubu University (2004)
Nagoya University (2003)

Principal Investigator

HORIO Fumihiko  Chubu University, College of Bioscience & Biotechnology, Professor, 応用生物学部, 教授 (20165591)

Co-Investigator(Kenkyū-buntansha) NISHIMURA Masahiko  Nagoya University, Graduate School of Medicine, Professor, 大学院・医学系研究科, 教授 (20073661)
IKEGAMI Hiroshi  Osaka University, Graduate School of Medicine, Associate Professor, 大学院・医学系研究科, 助教授 (20221062)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2004: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2003: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsType 2 diabetes / Disease gene / QTL analysis / High-fat diet / Nutritional Biochemistry / Genetics / 糖尿病モデルマウス / 糖尿病遺伝子
Research Abstract

The SMXA-5 mouse is one of the 26 SMXA recombinant inbred (RI) substrains that have been established from nondiabetic SMIJ and A/J strains. This mouse is a model for polygenic type 2 diabetes characterized by both moderate impairment of glucose tolerance and mild hyperinsulinemia. To dissect A/J-derived diabetogenic loci of SMXA-5 contributing to diabetes-related traits such as impaired glucose tolerance, hyperglycemia, hyperinsulinemia, and obesity, we attempted to analyze the quantitative trait loci (QTL) in (SM/J x SAM-5)F2 intercross mice fed a high-fat diet. A major QTL for body mass index, nonfasting blood glucose concentration, and glucose tolerance was mapped on Chr 2. This locus existed near D2Mit15, with the highest logarithm of odds (LOD) score, 12.5, for glucose concentration being recorded at 120 min in an intraperitoneal glucose tolerance test (IPGTT). We designated this QTL as t2dm2sa, for type 2 diabetes mellitus 2 in the SMXA RI strains. SM.A-t2dm2sa, a congenic strain that introgressed the A/J-derived t2dm2sa region into SM/J, exhibited overt impaired glucose tolerance and mild hyperinsulinemia under a high-fat diet. These results suggested that latent diabetogenic genes exist in the genomes of nondiabetic AM and SM/J mice, and that interaction between t2dm2sa and an unknown SM/J-derived locus except where t2dm2sa exists, elicits impaired glucose tolerance in SMXA-5 and SM.A-t2dm2sa mice. The dissection of these diabetogenic genes that have epistatic effects will contribute to the elucidation of the complex mechanisms underlying human type 2 diabetes.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (26 results)

All 2004 2003 Other

All Journal Article (25 results) Publications (1 results)

  • [Journal Article] SMXA-5 mouse as a diabetic model susceptible to feeding a high-fat diet.2004

    • Author(s)
      Kobayashi, M.
    • Journal Title

      Biosci.Biotechnol.Biochem. 68

      Pages: 226-230

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] リコンビナント・インブレッド系統マウスを用いた2型糖尿病原因遺伝子の探索2004

    • Author(s)
      小林美里
    • Journal Title

      肥満研究 10

      Pages: 81-83

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Anthocyanin enhances adiopocytokine secretion and adipocyte-specific gene expression in isolated rat adipocytes.2004

    • Author(s)
      Tsuda, T.
    • Journal Title

      Biochem.Biophys.Res.Commun. 316

      Pages: 149-157

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Characterization of diabetes-related traits in MSM and JF1 mice on hi-fat diet.2004

    • Author(s)
      Kobayashi, M.
    • Journal Title

      J.Nutr.Biochem. 15

      Pages: 614-621

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Suppressive effect of hot water extract od Adzuki beans (Vigna angularis) on hyperglycemia after sucrose loading in mice and diabetic rats.2004

    • Author(s)
      Itoh, T.
    • Journal Title

      Biosci.Biotechnol.Biochem. 68

      Pages: 2421-2426

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] SMXA-5 mouse as a diabetic model susceptible to feeding a high-fat diet.2004

    • Author(s)
      Kobayashi, M., Io, F., Kawai, T., Nishimura, M., Ohno, T., Horio, F
    • Journal Title

      Biosci.Biotechnol.Biochem. 68

      Pages: 226-230

    • NAID

      10013141392

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Anthocyanin enhances adiopocytokine secretion and adipocyte-specific gene expression in isolated rat adipocytes.2004

    • Author(s)
      Tsuda, T., Ueno, Y., Aoki, H., Koda, T., Horio, F., Takahashi, N., Kawada, T., Osawa, T.
    • Journal Title

      Biochem.Biophys.Res.Commun. 316

      Pages: 149-157

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Characterization of diabetes-related traits in MSM and JF1 mice on hi-fat diet.2004

    • Author(s)
      Kobayashi, M., Ohno, T., Tsuchiya, T., Horio, F.
    • Journal Title

      J.Nutr.Biochem. 15

      Pages: 226-230

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Suppressive effect of hot water extract od Adzuki beans (Vigna angularis) on hyperglycemia after sucrose loading in mice and diabetic rats.2004

    • Author(s)
      Itoh, T., Kita, N., Kurokawa, Y., Kobayashi, M., Horio, F.Furuichi, Y.
    • Journal Title

      Biosci.Biotechnol.Biochem. 68

      Pages: 2421-2426

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] SMXA-5 mouse as a diabetic model susceptible to feeding a high-fat diet.2004

    • Author(s)
      Kobayashi, M., Io, F., Kawai, T., Nishimura, M., Ohno, T., Horio, F
    • Journal Title

      Biosci.Biotechnol. Biochem. 68(1)

      Pages: 226-230

    • NAID

      10013141392

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Anthocyanin enhances adiopocytokine secretion and adipocyte-specific gene expression in isolated rat adipocytes.2004

    • Author(s)
      Tsuda, T., Ueno, Y., Aoki, H., Koda, T., Horio, F., Takahashi, N., Osawa, T.
    • Journal Title

      Biochem.Biophys.Res.Commun. 316(6)

      Pages: 149-157

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Characterization of diabetes-related traits in MSM and JF1 mice on high-fat diet.2004

    • Author(s)
      Kobayashi, M., Ohno, T., Tsuchiya, T., Horio, F.
    • Journal Title

      J.Nutr.Biochem. 15(10)

      Pages: 614-621

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Manganese levels in tissues of paraquat dised and/or magnesium restricted rats measured by an electron spin resonance method.2004

    • Author(s)
      Minakata, K., Horio, F., Saito, S., Watanabe, K., Suzuki, O.
    • Journal Title

      Jpn.J.Forensic Toxicol. 22(12)

      Pages: 193-195

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Suppressive effect of hot water extract od Adzuki beans (Vigna angularis) on hyperglycemia after sucrose loading in mice and diabetic rats.2004

    • Author(s)
      Itoh, T., Kita, N., Kobayashi, M., Horio, F., Furuich, Y.
    • Journal Title

      Biosci.Biotechnol.Biochem. 68(12)

      Pages: 2421-2426

    • Related Report
      2004 Annual Research Report
  • [Journal Article] リコンビナント・インブレッド系統マウスを用いた2型糖尿病原因遺伝子の探索2004

    • Author(s)
      小林美里, 井尾房代, 河合隆博, 大野民生, 西村正彦, 堀尾文彦
    • Journal Title

      肥満研究 10(4)

      Pages: 81-83

    • NAID

      10016998854

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Quantitative trait locus analysis of serum insulin, triglyceride, total cholesterol and phospholipid levels in the (SM/JxA/J)F2 mice.2003

    • Author(s)
      Anunciado, R.V.P.
    • Journal Title

      Exp.Anim. 52

      Pages: 37-42

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Combinations of non-diabetic parental genomes elicit impaired glucose tolerance in mouse SMXA RI strains.2003

    • Author(s)
      Kobayashi, M.
    • Journal Title

      Diabetes 52

      Pages: 180-186

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Changes in catecholamine metabolism by ascorbic acid deficiency in spontaneously hypertensive rats unable to synthesize ascorbic acid.2003

    • Author(s)
      Kawai, K.
    • Journal Title

      Life Sci. 72

      Pages: 1717-1732

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Characteristics of ascorbic acid metabolism in the scurvy-prone spontaneously hypertensive rat, SHR-od.2003

    • Author(s)
      Kawai, K.
    • Journal Title

      J.Nutr.Sci.Vitaminol. 49

      Pages: 1-6

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Dietary cyanidin 3-0-b-D-glucoside-rich purple corn color prevents, obesity and ameliorates hyperglycemia in mice.2003

    • Author(s)
      Tsuda T.
    • Journal Title

      J.Nutr. 133

      Pages: 2125-2130

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Quantitative trait locus analysis of serum insulin, triglyceride, total cholesterol and phospholipid levels in the (SM/JxA/J)F2 mice.2003

    • Author(s)
      Anunciado, R.V.P., Nishimura, M., Mori, M., Ishikawa, A., Tanaka, S., Horio, F., Ohno, T., Namikawa, T.
    • Journal Title

      Exp.Anim. 52

      Pages: 37-42

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Combinations of non-diabetic parental genomes elicit impaired glucose tolerance in mouse SMXA RI strains.2003

    • Author(s)
      Kobayashi, M., Ohno, T., Tsuji, A., Nishimura, M., Horio, F.
    • Journal Title

      Diabetes 52

      Pages: 180-186

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Changes in catecholamine metabolism by ascorbic acid deficiency in spontaneously hypertensive rats unable to synthesize ascorbic acid.2003

    • Author(s)
      Kawai, K., Ito, H., Kubota, H., Takemori, K., Makino, S., Horio, F.
    • Journal Title

      Life Sci. 72

      Pages: 1717-1732

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Characteristics of ascorbic acid metabolism in the scurvy-prone spontaneously hypertensive rat, SHR-od.2003

    • Author(s)
      Kawai, K., Nishio, N., Horio, F.
    • Journal Title

      J.Nutr.Sci.Vitaminol. 49

      Pages: 1-6

    • NAID

      10012072651

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Dietary cyanidin 3-O-b-D-glucoside-rich purple corn color prevents obesity and ameliorates hyperglycemia in mice.2003

    • Author(s)
      Tsuda T., Horio, F., Uchida, K., Aoki, H., Osawa, T.
    • Journal Title

      J.Nutr. 133

      Pages: 2125-2130

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Kobayashi, M., Io, F., Kawai, T., Nishimura, M., Ohno, T., Horio, F.: "SMXA-5 mouse as a diabetic model susceptible to feeding a high-fat diet"Biosci.Biotechnol.Biochem.. 68(1). 226-230 (2004)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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