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Chemical and biological studies of propolis and its origin plants

Research Project

Project/Area Number 15580111
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Food science
Research InstitutionUniversity of Shizuoka

Principal Investigator

KUMAZAWA Shigenori  University of Shizuoka, School of Food and Nutritional Sciences, Associate professor, 食品栄養科学部, 助教授 (10295561)

Co-Investigator(Kenkyū-buntansha) NAKAYAMA Tsutomu  University of Shizuoka, School of Food and Nutritional Sciences, Professor, 食品栄養科学部, 教授 (50150199)
SHIMOI Kayoko  University of Shizuoka, Department of Environmental Health Sciences, Professor, 環境科学研究所, 教授 (10162728)
NAKAMURA Jun  Tamagawa University, Research Institute, Associate Professor, 学術研究所, 助教授 (30256002)
Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2005: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2004: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2003: ¥1,100,000 (Direct Cost: ¥1,100,000)
Keywordspropolis / origin plant / antioxidant activity / metabolism / flavonoid / honeybee / 酵素阻害活性 / アレクリン / バッカリス
Research Abstract

Propolis is a resinous substance collected by honeybees from various plant sources. We studied the constituents and biological activities of propolis and its origin plants. In Japan, Brazilian propolis is widely used. To identify the plant origin of Brazilian propolis directly, we observed of honeybees in Minas Gerais State of Brazil and analyzed chemically the plant used by honeybees. We identified that plant origin of Brazilian propolis is Baccharis dracunculifolia. Further we examined the antioxidant activity of propolis from various areas of Japan. We identified the major constituents in ethanol extracts of propolis (EEP) by HPLC analysis by a photodiode array (PDA) and mass spectrometric (MS) detection, and quantitatively analyzed each component. EEP from Akita (Minamiakita) and Okinawa had relatively strong antioxidant activity, and was correlated with total polyphenol contents. Propolis from Akita (Minakiakita) contained large amounts of the antioxidative compounds, caffeic acid and phenethyl caffeate. Propolis from Okinawa had antioxidants not seen in propolis from other areas. These antioxidants were isolated and identified by spectroscopic methods to be novel prenylated flavonoids. Also concerning with Korean propolis, we examined the antioxidant activity. Further we investigated the absorption and metabolism of the components in propolis. We administered EEP orally to rats and analyzed their plasma and urine by HPLC with PDA and MS detection, and detected several flavonoids in it. On the other hand, we proved that honeybees use different origin plants by seasons.

Report

(4 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (41 results)

All 2005 2004 2003 Other

All Journal Article (31 results) Book (5 results) Publications (5 results)

  • [Journal Article] 生活習慣病におけるサプリメント-プロポリス2005

    • Author(s)
      熊澤 茂則
    • Journal Title

      成人病と生活習慣病 35・9

      Pages: 1067-1067

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 補完代替医療素材としてのプロポリス2005

    • Author(s)
      中村 純
    • Journal Title

      日本補完代替医療学会誌 2・1

      Pages: 45-57

    • NAID

      130000079416

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] プロポリスの多様性とその機能の利用2005

    • Author(s)
      中村 純
    • Journal Title

      食品衛生学雑誌 46・2

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Supplements in adult deseases2005

    • Author(s)
      Shigenori Kumazawa
    • Journal Title

      The Journal of Adult Diseases 35-9

      Pages: 1067-1067

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Propolis as a material for complementary and alternative medicine2005

    • Author(s)
      Jun Nakamura
    • Journal Title

      Japanese Journal of Complementary and Alternative Medicine 2-1

      Pages: 45-57

    • NAID

      130000079416

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Diversity of propolis and use of its function2005

    • Author(s)
      Jun Nakamura
    • Journal Title

      Japanese Journal of Food Hygienic 46-2

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] プロポリス2005

    • Author(s)
      熊澤 茂則
    • Journal Title

      成人病と生活習慣病 35(9)

      Pages: 1067-1067

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Antioxidant activity of propolis of various geographic origins2004

    • Author(s)
      Shigenori Kumazawa
    • Journal Title

      Food Chemistry 84・3

      Pages: 329-339

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] A new prenylated flavonoid from propolis collected in Okinawa, Japan2004

    • Author(s)
      Shigenori Kumazawa
    • Journal Title

      Biosciences, Biotechnology, and Biochemistry 68・1

      Pages: 260-262

    • NAID

      10013141519

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Antioxidant activity and constituents of propolis collected in various areas of Japan2004

    • Author(s)
      Tomoko Hamasaka
    • Journal Title

      Food Science and Technology Research 10・1

      Pages: 86-92

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Identification of metabolites in plasma and urine of Uruguayan propolis-treated rats2004

    • Author(s)
      Shigenori Kumazawa
    • Journal Title

      Journal of Agricultural and Food Chemistry 52・10

      Pages: 3083-3088

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Antioxidant activity and constituents of propolis collected in various areas of Korea2004

    • Author(s)
      Mok-Ryeon Ahn
    • Journal Title

      Journal of Agricultural and Food Chemistry 52・24

      Pages: 7286-7292

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] ブラジル産プロポリスに含まれる成分と起源植物2004

    • Author(s)
      熊澤 茂則
    • Journal Title

      Foods and Food Ingredients Journal of Japan 209・2

      Pages: 132-140

    • NAID

      40006125531

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 沖縄産プロポリスに含まれる新規成分2004

    • Author(s)
      熊澤 茂則
    • Journal Title

      FOOD Style 21 8・5

      Pages: 1-5

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] プロポリスに含まれるフラボノイドの吸収・代謝2004

    • Author(s)
      熊澤 茂則
    • Journal Title

      ミツバチ科学 25・4

      Pages: 152-158

    • NAID

      120006868563

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Antioxidant activity of propolis of various geographic origins2004

    • Author(s)
      Shigenori Kumazawa
    • Journal Title

      Food Chemistry 84-3

      Pages: 329-339

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] A new prenylated flavonoid from propolis collected in Okinawa, Japan2004

    • Author(s)
      Shigenori Kumazawa
    • Journal Title

      Biosciences, Biotechnology, and Biochemistry 68-1

      Pages: 260-262

    • NAID

      10013141519

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Antioxidant activity and constituents of propolis collected in various areas of Japan2004

    • Author(s)
      Tomoko Hamasaka
    • Journal Title

      Food Science and Technology Research 10-1

      Pages: 86-92

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Identification of metabolites in plasma and urine of Uruguayan propolis-treated rats2004

    • Author(s)
      Shigenori Kumazawa
    • Journal Title

      Journal of Agricultural and Food Chemistry 52-10

      Pages: 3083-3088

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Antioxidant activity and constituents of propolis collected in various areas of Korea2004

    • Author(s)
      Mok-Ryeon Ahn
    • Journal Title

      Journal of Agricultural and Food Chemistry 52-24

      Pages: 7286-7292

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Constituents in Brazilian propolis and its plant of origin2004

    • Author(s)
      Shigenori Kumazawa
    • Journal Title

      Foods and Food Ingredients Journal of Japan 209-2

      Pages: 132-140

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] New compounds in Okinawa propolis2004

    • Author(s)
      Shigenori Kumazawa
    • Journal Title

      FOOD Style 21 8-5

      Pages: 1-5

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Absorption and metabolism of flavonoids in propolis2004

    • Author(s)
      Shigenori Kumazawa
    • Journal Title

      Honeybee Science 25-4

      Pages: 152-158

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Direct evidence for the plant origin of Brazilian propolis by the observation of honeybee behavior and phytochemical analysis2003

    • Author(s)
      Shigenori Kumazawa
    • Journal Title

      Chemical and Pharmaceutical Bulletin 51・6

      Pages: 740-742

    • NAID

      110003615388

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Direct evidence for the plant origin of Brazilian propolis by the observation of honeybee behavior and phytochemical analysis2003

    • Author(s)
      Shigenori Kumazawa
    • Journal Title

      Chemical and Pharmaceutical Bulletin 51-6

      Pages: 740-742

    • NAID

      110003615388

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The functional organization of resin work in honeybee colonies

    • Author(s)
      Jun Nakamura
    • Journal Title

      Behavioral Ecology and Sociobiology (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Antioxidant activity and constituents of propolis collected in various areas of China

    • Author(s)
      Mok-Ryeon Ahn
    • Journal Title

      Food Chemistry (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] A new chalcone from propolis collected on Jeju island, Korea

    • Author(s)
      Shigenori Kumazawa
    • Journal Title

      Food Science and Technology Reserch (印刷中)

    • NAID

      10017620196

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The functional organization of resin work in honeybee colonies

    • Author(s)
      Jun Nakamura
    • Journal Title

      Behavioral Ecology and Sociobiology (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Antioxidant activity and constituents of propolis collected in various areas of China

    • Author(s)
      Mok-Ryeon Ahn
    • Journal Title

      Food Chemistry (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] A new chalcone from propolis collected on Jeju island, Korea

    • Author(s)
      Shigenori Kumazawa
    • Journal Title

      Food Science and Technology Research (in press)

    • NAID

      10017620196

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] がん予防食品開発の新展開2005

    • Author(s)
      熊澤 茂則
    • Total Pages
      354
    • Publisher
      シーエムシー出版
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] Japanese Journal of Food Hygienic,46-22005

    • Author(s)
      Shigenori Kumazawa
    • Publisher
      Diversity of propolis and use of its function
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] Japanese Journal of Food Hygienic, 46-22005

    • Author(s)
      Shigenori Kumazawa
    • Publisher
      Diversity of propolis and use of its function
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] がん予防食品開発の新展開-予防医学におけるバイオマーカーの評価システム-2005

    • Author(s)
      熊澤 茂則
    • Total Pages
      354
    • Publisher
      シーエムシー出版
    • Related Report
      2005 Annual Research Report
  • [Book] 医療従事者のための機能性食品ガイド2004

    • Author(s)
      熊澤 茂則
    • Total Pages
      511
    • Publisher
      講談社
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Publications] Shigenori Kumazawa: "Direct evidence for the plant origin of Brazilian propolis by the observation of honeybee behavior and phytochemical analysis"Chemical and Pharmaceutical Bulletin. 51・6. 740-742 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Shigenori Kumazawa: "Antioxidant activity of propolis of various geographic origins"Food Chemistry. 84・3. 329-339 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Shigenori Kumazawa: "A new prenylated flavonoid from propolis collected in Okinawa, Japan"Biosciences, Biotechnology, and Biochemistry. 68・1. 260-262 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Tomoko Hamasaka: "Antioxidant activity and constituents of propolis collected in various areas of Japan"Food Science and Technology Research. 10・1. 86-92 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 熊澤茂則: "ブラジル産プロポリスに含まれる成分と起源植物"Foods and Food Ingredients Journal of Japan. 209・2. 132-140 (2004)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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