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Purification and identification of the growth promoting substance from corn fiber and its promotion mechanisms on useful mushroom productions

Research Project

Project/Area Number 15580151
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 林産科学・木質工学
Research InstitutionKinki University

Principal Investigator

TERASHITA Takao  Kinki University, Fac.of Agriculture, Professor, 農学部, 教授 (90088203)

Co-Investigator(Kenkyū-buntansha) KITAMOTO Yutaka  Tottri University, Fac.of Agriculture, Professor, 農学部, 教授 (10032294)
SUZUKI Akira  Chiba University, Fac.of Education, Professor, 教育学部, 教授 (50110797)
YOSHIKAWA Kentaro  Kinki University, Fac.of Agriculture, Associate Pro., 農学部, 助教授 (20088206)
SHIRASAKA Norifumi  Kinki University, Fac.of Agriculture, Lecture, 農学部, 講師 (20268452)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2004: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2003: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsMushroom / Mushroom Cultivation / Corn fiber / Corn fiber Waste Product / Growth Promoting Substance for mushroom
Research Abstract

Corn fiber (CNF) is an abundant by-product of the wet corn milling process in the production of corn starch. We have shown that the hot water soluble fraction (HWSF) from CNF has a promoting effect on the mycelial growth of various edible mushrooms including mycorrhizal mushroom such as Tricholoma matsutake and so on. Moreover, fruit-body yield increased at 1.2-1.6 times by using CNF, and the cultivation period was dramatically shortened compared to that of the control without CNF. To reveal the promoting mechanisms, the effect of CNF-HWSF on the stimulation of extracellular enzymes was examined. As a result, the production of carbohydrases such as amylase, CMCase and xylanase were markedly enhanced by the addition of low molecular weight fractions (less than M.W.500) prepared from CNF-HWSF. The enzymatic stimulations and enhancement of mycelial growth appeared during the about same time on the culture period. Furthermore, fraction less than the M.W.500 was separated by gel filtration into 5 fractions, and the effect of each fraction was investigated. The promoting effects were indicated even on a fraction D has no sugars and amino acids in CNF-HWSF, From these results, the promoting effect by CNF-HWSF seems to be a 2 step reactions. The first step could be achieve by rich nutrients such as mono- and ologo-saccharides and free amino acids from CNF-HWSF. The second step considered that the marked promoting effect was caused by the stimulation of extracellular enzymes. Now, we are in the improvement stage for practical application of CNF as a growth substrate using sawdust-based cultivation. Further attempts will be made to investigate the isolation and the characterization of promoting substances in CNF-HWSF to clarify the promotion mechanisms on edible mushrooms.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report

Research Products

(17 results)

All 2004 2003 Other

All Journal Article Publications

  • [Journal Article] Acceleration of the fruit-body formation of edible mushrooms by the extract from corn fiber2004

    • Author(s)
      Yasue Arai
    • Journal Title

      Mushroom Science and Biotechnology 12・4

      Pages: 171-177

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Acceleration of the fruit-body formation of edible mushrooms by the extract From corn fiber2004

    • Author(s)
      Yasue ARAI
    • Journal Title

      Mushroom Science and Biotechnology 12(4)

      Pages: 171-177

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 食用きのこ菌床栽培への廃棄物コーンファイバー(CNF)の利用2003

    • Author(s)
      荒井 康恵
    • Journal Title

      日本応用きのこ学会誌 11・1

      Pages: 17-23

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Promotive effect of the hot water-soluble fraction from corn fiber on vegetative Mycelial growth in edible mushrooms2003

    • Author(s)
      Yasue Arai
    • Journal Title

      J.Wood Science 49・5

      Pages: 437-443

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Waste by-product, Corn fiber (CNF) for use as a substrate in the sawdust-based cultivation of edible mushrooms2003

    • Author(s)
      Yasue ARAI
    • Journal Title

      Mushroom Science and Biotechnology 11(1)

      Pages: 17-23

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Promotive effect of the hot water soluble fraction from corn fiber on vegetative mycelial growth in edible mushrooms2003

    • Author(s)
      Yasue ARAI
    • Journal Title

      J.Wood Science 49(5)

      Pages: 437-443

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Promotive effect of the hot water-soluble fraction from corn fiber on vegetative Mvcelial growth in edible mushrooms2003

    • Author(s)
      Yasue Arai
    • Journal Title

      J.Wood Science 49・5

      Pages: 437-443

    • Related Report
      2004 Annual Research Report
  • [Journal Article] The stimulation of extracellular carbohydrases of edible mushrooms By the hot water soluble fraction from corn fiber

    • Author(s)
      Yasue Arai
    • Journal Title

      Mycoscience (In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] コーンファイバーに含まれるきのこの子実体形成促進物質の単離と構造

    • Author(s)
      寺下 隆夫
    • Journal Title

      日本きのこ学会誌 (発表予定)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] コーンファイバー添加培地で栽培された食用きのこ類の機能性評価

    • Author(s)
      白坂 憲章
    • Journal Title

      日本きのこ学会誌 (発表予定)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The sutimulation of extracellular carbohydrases of edible mushrooms by hot water soluble fraction from corn fiber

    • Author(s)
      Yasue ARAI
    • Journal Title

      Mycoscience 46(4)(in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Purification procedures and chemical stracture of promoting substance on fruit-body formation of mushrooms from corn fiber

    • Author(s)
      Takao TERASHITA
    • Journal Title

      Mushroom Scince and Biotechnology (in preparation)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Fanctional valuation for edible mushrooms cultured on corn fiber medium

    • Author(s)
      Norifumi SHIRASAKA
    • Journal Title

      Mushroom Science and Biotechnology (in preparation)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The stimulation of extracellular carbohydrases of edible mushrooms By the hot water soluble fraction from corn fiber

    • Author(s)
      Yasue Arai
    • Journal Title

      Mycoscience (In press)

    • Related Report
      2004 Annual Research Report
  • [Publications] 荒井 康恵: "食用きのこ菌菌床栽培への廃棄物コーンファイバー(CNF)の利用"日本応用きのこ学会誌. 11・1. 17-23 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yasue Arai: "Promotive effect of the hot water-soluble fraction from corn fiber on vegetative mycelium in edible mushrooms"J.Wood Science. 49・5. 437-443 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Takao Terashita: "Effect of the hot water-soluble fraction from corn fiber on fruit-body growth in Lentinula edodes and Flammulina velutipes"Mushroom Sci.and Biotechnol.. (発表予定).

    • Related Report
      2003 Annual Research Report

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Published: 2003-03-31   Modified: 2016-04-21  

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