Purification and identification of the growth promoting substance from corn fiber and its promotion mechanisms on useful mushroom productions
| Project/Area Number |
15580151
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
林産科学・木質工学
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| Research Institution | Kinki University |
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Principal Investigator |
TERASHITA Takao Kinki University, Fac.of Agriculture, Professor, 農学部, 教授 (90088203)
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| Co-Investigator(Kenkyū-buntansha) |
KITAMOTO Yutaka Tottri University, Fac.of Agriculture, Professor, 農学部, 教授 (10032294)
SUZUKI Akira Chiba University, Fac.of Education, Professor, 教育学部, 教授 (50110797)
YOSHIKAWA Kentaro Kinki University, Fac.of Agriculture, Associate Pro., 農学部, 助教授 (20088206)
SHIRASAKA Norifumi Kinki University, Fac.of Agriculture, Lecture, 農学部, 講師 (20268452)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2004: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2003: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Mushroom / Mushroom Cultivation / Corn fiber / Corn fiber Waste Product / Growth Promoting Substance for mushroom |
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| Research Abstract |
Corn fiber (CNF) is an abundant by-product of the wet corn milling process in the production of corn starch. We have shown that the hot water soluble fraction (HWSF) from CNF has a promoting effect on the mycelial growth of various edible mushrooms including mycorrhizal mushroom such as Tricholoma matsutake and so on. Moreover, fruit-body yield increased at 1.2-1.6 times by using CNF, and the cultivation period was dramatically shortened compared to that of the control without CNF. To reveal the promoting mechanisms, the effect of CNF-HWSF on the stimulation of extracellular enzymes was examined. As a result, the production of carbohydrases such as amylase, CMCase and xylanase were markedly enhanced by the addition of low molecular weight fractions (less than M.W.500) prepared from CNF-HWSF. The enzymatic stimulations and enhancement of mycelial growth appeared during the about same time on the culture period. Furthermore, fraction less than the M.W.500 was separated by gel filtration into 5 fractions, and the effect of each fraction was investigated. The promoting effects were indicated even on a fraction D has no sugars and amino acids in CNF-HWSF, From these results, the promoting effect by CNF-HWSF seems to be a 2 step reactions. The first step could be achieve by rich nutrients such as mono- and ologo-saccharides and free amino acids from CNF-HWSF. The second step considered that the marked promoting effect was caused by the stimulation of extracellular enzymes. Now, we are in the improvement stage for practical application of CNF as a growth substrate using sawdust-based cultivation. Further attempts will be made to investigate the isolation and the characterization of promoting substances in CNF-HWSF to clarify the promotion mechanisms on edible mushrooms.
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Report
(3 results)
Research Products
(17 results)
