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Analyses of factors controlling infection and development of fish-infecting myxosporeans

Research Project

Project/Area Number 15580159
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General fisheries
Research InstitutionThe University of Tokyo

Principal Investigator

YOKOYAMA Hiroshi  The University of Tokyo, Graduate School of Agricultural and Life Sciences, Assistant, 大学院・農学生命科学研究科, 助手 (70261956)

Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 2004: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2003: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsMyxosporean / Mucus / Lectin / Emaciation disease / Fish parasite / 放線胞子虫 / 体表粘液 / サクラマス
Research Abstract

It has been reported that actinospores, the infective stage of myxosporeans (mainly freshwater species), release the sporoplasms in contact with fish skin mucus and then initiate the invasion into fish hosts. Recently, fish-to-fish transmission of Enteromyxum leei from the intestine of marine fishes has been demonstrated. Therefore, in the present study, I aimed to clarify the characteristics of fish mucus inducing sporoplasm release of freshwater actinospores, and to evaluate the infectivity and the attachment ability of marine myxosporean trophozoites to fish intestinal mucus.
Sporoplasm release of actinospores of Myxobiolus areticus infecting salmonid fishes was investigated in relation to skin mucus. Skin mucus from masu salmon (a susceptible fish) stimulated sporoplasm releases dose-dependently ; percentage of sporoplasm release reached 60 % at 1.0 mg-protein/ml. On the other hand, in non-susceptible fishes, percentages of sporoplasm release were 37 % and 20 % in sockeye salmon and goldfish, respectively. It was also shown that several commercial lectins (Con A, etc.) prohibited but some (RCA I, etc.) activated sporoplasm release of actinospores, suggesting the involvement of sugars in chemoreception of actinospores. Further, isolated sporoplasms of actinospores successfully transmitted naive fish by an intrapentoneal injection.
Morphological and molecular analyses showed that a myxosporean from the intestine of tiger puffer is identified as E.leei. PCR for detection of E.leei was developed. No difference was observed in attachment to intestinal mucus and susceptibility among marine fish species, indicating that E.leei has a broad host range.
It is concluded that fish mucus (from skin or intestine) plays an important role in the invasion of myxosporeans, and that sugar-lectin interaction is one of the factors controlling myxosporean infection.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (8 results)

All 2005 2004 2003 Other

All Journal Article (8 results)

  • [Journal Article] Development of a PCR-based method for the detection of the enteric myxozoans causing the emaciation disease of cultured tiger puffer.2005

    • Author(s)
      Yanagida T., M.A.Freeman, Y.Nomura, I.Takami, Y.Sugihara, H.Yokoyama, K.Ogawa
    • Journal Title

      Fish Pathology 40(印刷中)

    • NAID

      10019225072

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Development of a PCR-based assay for the detection of enteric myxozoans causing the emaciation disease of cultured tiger puffer.2005

    • Author(s)
      Yanagida T., M.A.Freeman, Y.Nomura, I.Takami, Y.Sugihara, H.Yokoyama, K.Ogawa
    • Journal Title

      Fish Pathology 40・1(印刷中)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Molecular and morphological redescriptions of enteric myxozoans, Enteromyxum leei (formerly Myxidium sp.TP) and Enteromyxum fugu comb.n.(syn.Myxidium fugu) from cultured tiger puffer.2004

    • Author(s)
      Yanagida T., Y.Nomura, T.Kimura, Y.Fukuda, H.Yokoyama, K.Ogawa
    • Journal Title

      Fish Pathology 39

      Pages: 137-143

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Molecular and morphological redescriptions of enteric myxozoans, Enteromyxum leei (formerly Myxidium sp.TP) and Enteromyxum fugu comb.n.(syn.Myxidium fugu) from cultured tiger puffer.2004

    • Author(s)
      Yanagida T., Y.Nomura, T.Kimura, Y.Fukuda, H.Yokoyama, K.Ogawa
    • Journal Title

      Fish Pathol. 39-3

      Pages: 137-143

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Molecular and morphological redescriptions of enteric myxozoans, Enteromyxum leei(formerly Myxidium sp. TP) and Enteromyxum fugu comb.n.(syn.Myxidium fugu) from cultured tiger puffer.2004

    • Author(s)
      Yanagida T., Y.Nomura, T.Kimura, Y.Fukuda, H.Yokoyama, K.Ogawa
    • Journal Title

      Fish Pathology 39・3

      Pages: 137-143

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 魚類に寄生する粘液胞子虫の生活環と起源2003

    • Author(s)
      横山 博
    • Journal Title

      原生動物学雑誌 37

      Pages: 1-9

    • NAID

      130007430820

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Life cycle and evolutionary origin of myxozoan parasites of fishes.2003

    • Author(s)
      Hiroshi Yokoyama
    • Journal Title

      Jpn.J.Protozool. 37-1

      Pages: 1-9

    • NAID

      130007430820

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Development of a PCR-based method for the detection of the enteric myxozoans causing the emaciation disease of cultured tiger puffer.

    • Author(s)
      Yanagida T, M.A.Freeman, Y.Nomura, I.Takami, Y Sugihara, H.Yokoyama, K.Ogawa
    • Journal Title

      Fish Pathol. 41-1 (in press)

    • NAID

      10019225072

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary

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Published: 2003-04-01   Modified: 2016-04-21  

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