| Project/Area Number |
15580161
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General fisheries
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| Research Institution | Nagoya University |
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Principal Investigator |
OMURA Yuri Nagoya University, Graduate School of Bioagricultural Sciences, Assist.Professor, 大学院・生命農学研究科, 助手 (50023479)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2004: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2003: ¥2,800,000 (Direct Cost: ¥2,800,000)
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| Keywords | eel Anguilla japonica / flounder Paralichthys olivaceus / trout Oncorhynchus mykiss / retinal rod cells / pineal photoreceptor cells / PCNA / BrdU / immunocytochemistry / ニホンウナギ Anguilla japonica / ヒラメ Paralichthys olivaceus / ニジマス Oncorhynchus mykiss / 網膜桿体細胞 / 増殖細胞核抗原 (PCNA) / BrdU (Bromodioxyuridine) |
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| Research Abstract |
1)In parallel with histological observation on the increase in number of the rod cells, the distribution of proliferating cell nuclear antigen (PCNA)-immunoreactive cells was investigated in the mature retina of the teleost fish. A great number of PCNA-immunoreactive cells were demonstrated in both the outer and inner nuclear layers in the adult retina of the river eel Anguilla japonica migrating thousands of kilometers to breed in the ocean, and throughout the outer nuclear layer in the juvenile retina of the lefteye flounder Paralichthys olivaceus adapted to the benthic habitat and nocturnal feeding. These results suggest that the retinal rod cells proliferate throughout their life, following migration of the rod precursors from the progenitor clusters in the inner nuclear layer in the eel and from the basal to the apical region in the outer nuclear layer in the flounder.
2)Together with opsin-immunoreactive photoreceptor outer segments, a tremendous number of PCNA-immunoreactive cells were demonstrated throughout the end-vesicle and stalk in the pineal organ of the 2 years-old rainbow trout Oncorhynchus mykiss lacking a circadian clock of melatonin synthesis. No or little coexistence of PCNA and opsin was demonstrated, although the close approaches of opsin-positive photoreceptor outer segments and PCNA-immunoreactive cells were found sometimes. These findings suggested that the pineal photoreceptor cells also proliferate in the adult rainbow trout, thus keeping highly sensitive signal-transduction mechanisms for active melatonin synthesis.
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