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Respiratory burst activity of ayu (Plecoglossus altivelis) neutrophils : comparison with other fresh water fish

Research Project

Project/Area Number 15580173
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General fisheries
Research InstitutionNihon University

Principal Investigator

MORITOMO Tadaaki  Nihon University, College of Bioresource Sciences, Associate professor, 生物資源科学部, 助教授 (20239677)

Co-Investigator(Kenkyū-buntansha) NAKANISHI Teruyuki  Nihon University, College of Bioresource Sciences, Professor, 生物資源科学部, 教授 (00322496)
井上 裕基  日本大学, 生物資源科学部, 助手 (50333571)
Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2005: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2004: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2003: ¥2,300,000 (Direct Cost: ¥2,300,000)
Keywordsayu / neutrophil / reactive oxygen species / carp / fish / priming / 炎症 / NDDPHオキシダーゼ / フグ / ニジマス / 炎症性サイトカイン / NADPH酸化酵素
Research Abstract

Neutrophils of vertebrates undergo a respiratory burst activity (RBA) as a defense mechanism against bacterial infections. We report here that ayu (Plecoglossus altivelis) have unusually high RBA even when they are in a healthy condition. Kidney (major hematopoietic organ in teleost fish) and blood leukocytes were obtained from ayu, rainbow trout (Oncorhynchus mykiss), carp (Cyprinus carpio), eel (Anguilla japonica), and pond smelt (Hypomesus nipponensis). Neutrophil RBA was measured by flow cytometry using dihydrorhodamine after stimulation with phorbol myristate acetate (PMA). The amount of RBA of neutrophils from both blood and kidney was significantly higher in ayu than in the other species (e.g., the fluorescence intensity of ayu blood neutrophils was about 3 to 7 times higher than that from trout and carp, and that of ayu kidney neutrophils was 2 to 19 times higher than that of rainbow-trout, carp, eel, and pond-smelt). This unique character of ayu neutrophils was invariable even … More at different ages, locations, and sex-maturation stages.
To understand this unique character of ayu neutrophils, the RBAs of resting and inflammatory neutrophils of ayu and carp were compared. Inflammation was induced in the peritoneal cavity by injecting killed-bacteria. The inflammatory neutrophils were obtained from peritoneal cavity. Resting neutrophils were obtained from blood and kidney of non-injected fish.
In carp, the RBA of inflammatory neutrophils was much higher than that of resting neutrophils. On the other hand, in ayu no significant difference was observed. The RBA of neutrophils was already high in the kidney stock. The process of inflammation did not further enhance RBA. In addition to PMA, other stimulants (zymosan, opsonized-zymosan, and zymosan treated serum) were used to measure RBA. Even with these stimulants, the RBA of inflammatory neutrophils was always higher than that of kidney neutrophils in carp. On the other hand, no significant difference was observed in ayu. These results indicate ayu neutrophils have spontaneously activated characteristics with the respect to the ROS generation even in the resting state.
At this point, it is not clear why ayu have such unique neutrophils with respect to the ROS generation. One possibility is that the high respiratory burst activity is related to the short life span of ayu (essentially one year). With such a short life span, it is likely that the innate immunity by spontaneously activated neutrophils is more important than acquired immunity. Less

Report

(4 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (14 results)

All 2005 2004 2003 Other

All Journal Article (10 results) Publications (4 results)

  • [Journal Article] Comparison of respiratory burst activity of inflammatory neutrophils in ayu (Plecoglossus altivelis) and carp (Ciprinus carpio)2005

    • Author(s)
      Ken Serada, Tadaaki Moritomo, Kyosuke Teshirogi, Takuya Itou, Takashi Shibashi, Yuuki Inoue, Teruyuki Nakanishi
    • Journal Title

      Fish & Shellfish Immunology 19

      Pages: 363-373

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Comparison of respiratory burst activity of inflammatory neutrophils in ayu (Plecoglossus altivelis) and carp (Cyprinus carpio)2005

    • Author(s)
      Ken Serada, Tadaaki Moritomo, Kyosuke Teshirogi, Takuya Itou, Takashi Shibashi, Yuuki Inoue, Teruyuki Nakanishi
    • Journal Title

      Fish & Shellfish Immunology 19

      Pages: 363-373

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Comparison of respiratory burst activity of inflammatory neutrophils in ayu (Plecoglossus altivelis) and carp (Cyprinus carpio).2005

    • Author(s)
      Serada K., Moritomo T., Teshirogi K., Itou T., Shibashi T., Inoue Y., Nakanishi T.
    • Journal Title

      Fish & Shellfish Immunology 19

      Pages: 363-373

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Comparison of respiratory burst activity of inflammatory neutrophils in ayu (Plecoglossus altivelis) and carp (Cyprinus carpio).2005

    • Author(s)
      Serada K., Moritomo T., Teshirogi K., Itou T., Shibashi T., Inoue Y., Nakanishi
    • Journal Title

      Fish & Shellfish Immunology (In press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Molecular cloning and sequencing of Japanese pufferfish (Takifugu rubripes) NADPH oxidase cDNAs2004

    • Author(s)
      Yuuki Inoue, Yuuki Suenaga, Yasutoshi Yshiura, Tadaaki Moritomo, Mitsuru Ototake, Teruyuki Nakanishi
    • Journal Title

      Developmental & Comparative Immunology 28

      Pages: 911-925

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Molecular cloning and sequencing of Japanese pufferfish (Takifugu rubripes) NADPH oxidase cDNAs2004

    • Author(s)
      Yuuki Inoue, Yuuki Suenaga, Yasutoshi Yoshiura, Tadaaki Moritomo, Mitsuru Ototake, Teruyuki Nakanishi
    • Journal Title

      Developmental & Comparative Immunology 28

      Pages: 911-925

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Molecular cloning and sequencing of Japanese pufferfish (Takifugu rubripes) NADPH oxidase cDNAs.2004

    • Author(s)
      Inoue Y., Suenaga Y., Yoshiura Y., Moritomo T., Ototake M., Nakanishi T.
    • Journal Title

      Developmental & Comparative Immunology 28(9)

      Pages: 911-925

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Flow cytometric analysis of the neutrophil respiratory burst of ayu, Plecoglossus altivelis : comparison with other fresh water fish.2003

    • Author(s)
      Tadaaki Moritomo, Ken Serata, Kyousuke Teshirogi, Hideaki Aikawa, Yuuki Inoue, Takuya Itou, Teruyuki Nakanishi
    • Journal Title

      Fish & Shellfish Immunology 15

      Pages: 29-38

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Flow cytometric analysis of the neutrophil respiratory burst of ayu, Plecoglossus altivelis : comparison with other fresh water fish2003

    • Author(s)
      Tadaaki Moritomo, Ken Serata, Kyousuke Teshirogi, Hideaki Aikawa, Yuuki Inoue, Takuya Itou, Teruyuki Nakanishi
    • Journal Title

      Fish & Shellfish Immunology 15

      Pages: 29-38

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Demonstration of hematopoietic stem cells in ginbuna carp (Carassius auratus langsdorfii) kidney

    • Author(s)
      Isao Kobayashia, Mayuko Sekiyaa, Tadaaki Moritomoa, Mituru Ototakeb, Teruyuki Nakanishia
    • Journal Title

      Developmental & Comparative Immunology (In press)

    • Related Report
      2005 Annual Research Report
  • [Publications] Tadaaki Moritomo, Ken Serataa, Kyousuke Teshirogib, Hideaki Aikawac, Yuuki Inouea, Takuya Itoua, Teruyuki Nakanishia: "Flow cytometric analysis of the neutrophil respiratory burst of ayu, Plecoglossus altivelis : comparison with other freshwater fish"Fish & Shellfish Immunology. 15. 29-38 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Tadaaki Moritomo, Naoko Asakura, Mayuko Sekiya, Mituru Ototake, Yuuki Inoue, Teruyuki Nakanishi: "Cell culture of clonal ginbuna crucian carp hematopoietic cells : differentiation of cultured cells into erythrocytes in vivo"Developmental & Comparative immunology. In press. (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yuuki INOUE, Yuuki SUENAGA, Yasutoshi YOSHIURA, Tadaaki MORITOMO, Mitsuru OTOTAKE, Teruyuki NAKANSHI: "Molecular Cloning and Sequencing of Japanese pufferfish (Takifugurubripes) NADPH oxidase cDNAs"Developmental & Comparative Immunology. In press.

    • Related Report
      2003 Annual Research Report
  • [Publications] Rahman, M.H., M.Ototake, T.Nakanishi: "Water soluble adjuvants enhance the protective effect of anti Flavobacterium psychrophilum vaccines in ayu, Plecoglossus altivelis."Fish Pathology. 38(4). 171-176 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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