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The sensitivity to endocrine disruptors and the establishment of sensitive bioassay for endocrine disruptors in poultry

Research Project

Project/Area Number 15580232
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Zootechnical science/Grassland science
Research InstitutionNiigata University

Principal Investigator

SUGIYAMA Toshie  Niigata University, Institute of Science and Technology, Assistant, 自然科学系, 助手 (10272858)

Co-Investigator(Kenkyū-buntansha) KUSUHARA Seiji  Niigata University, Institute of Science and Technology, Professor, 自然科学系, 教授 (80018788)
NISHIMURA Kouichi  Niigata University, National Institute of Livestock and Grassland Science Research, Manager, 畜産草地研究所, 主任研究員
Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2005: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2004: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2003: ¥1,600,000 (Direct Cost: ¥1,600,000)
Keywordschicken / Japanese quail / endocrine disruptor / sensitivity / bioassay / medullary bone / receptor / ビテロゲニン / バイオアッセイ系 / ダイオキシン受容体 / DDE / ダイオキシン / 卵管
Research Abstract

1.Aromatic hydrocarbon receptor (AhR ; dioxin receptor) mRNA expression with aging.
We detected the expression of AhR mRNA in various tissues of female chickens with aging (0 to 390 days old) by RT-PCR. As a result, AhR mRNA was detected in gizzard, liver, lung, kidney and heart during all stages. However, the oviduct and bone marrow of female chickens represented no AhR mRNA at 0-day stage and less at 30-90-day stages, respectively. These results suggest that the sensitivities of oviduct and bone marrow to dioxin are altered according to the age of female chickens.
2.Expression and localization of AhR in oviduct of laying chickens.
AhR expression was detected in magnum, isthmus and shell gland of oviducts by western blotting analysis. Especially in isthmus and shell gland, AhR was strongly localized in the cytoplasm of their epithelial cells. These results suggest that dioxin directly disrupts the metabolism of isthmus and shell gland, resulting in the eggshell weakness.
3.In Vivo bioassa … More y of endocrine disruptors using avian medullary bone.
The treatment of male Japanese quail with DDE (10mg/day) for 7 days induced medullary bone formation in marrow cavities of femurs. Concomitantly, the treatment increased the serum vitellogenin levels. These results suggest that avian medullary bone formation is superior as in vivo bioassay model of endocrine disruptors.
4.Expression and localization of endocrine disrupter receptors in avian medullary bone.
Estrogen receptor (α and β) and androgen receptor are well known as receptors for endocrine disruptor. In the present study by in situ hybridization and immunohistochemical analyses, both receptors (especially α subtype in estrogen receptor) were localized in osteoblasts of chicken medullary bone. These results suggest that endocrine disruptors directly act on osteoblastic bone formation.
5.RT-PCR analysis of vitellogenin mRNA expression in avian liver.
We tried to detect vitellogenin mRNA expression in liver of male quails treated with estrogen receptor agonists. As a result, vitellogenin mRNA expression was induced by estrogen receptor α agonist but not estrogen receptor β agonist. This indicates that vitellogenin synthesis in liver is stimulated through estrogen receptor α. Less

Report

(4 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (13 results)

All 2006 2005 2004 Other

All Journal Article (12 results) Publications (1 results)

  • [Journal Article] Expression and localization of estrogen receptor α and β mRNA in medullary bone of laying hens2006

    • Author(s)
      T.Imamura, T.Sugiyama, S.Kusuhara
    • Journal Title

      Animal Science Journal 77・2

      Pages: 223-229

    • NAID

      10021222023

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Expression and localization of estrogen receptor α and β mRNA in medullary bone of laying hens.2006

    • Author(s)
      T.Imamura, T.Sugiyama, S.Kusuhara
    • Journal Title

      Animal Science Journal 77(2)

      Pages: 223-229

    • NAID

      10021222023

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 鳥類カルシウム代謝における骨髄骨の形成と吸収2005

    • Author(s)
      杉山稔恵
    • Journal Title

      日本家禽学会誌 42・J4

    • NAID

      10017096905

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Effects of endocrine disruptors on avian medullary bone formation2005

    • Author(s)
      T.Sugiyama, N.Sakamoto, K.Nishimura, S.Kusuhara
    • Journal Title

      Bone 36・Supple 2

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Medullary bone formation and resorption in avian calcium metabolism.2005

    • Author(s)
      T.Sugiyama
    • Journal Title

      Japanese Journal of Poultry Science 42(J4)

    • NAID

      10017096905

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Effects of endocrine disruptors on avian medullary bone formation.2005

    • Author(s)
      T.Sugiyama, N.Sakamoto, K.Nishimura, S.Kusuhara
    • Journal Title

      Bone 36(Supplement 2)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 鳥類カルシウム代謝における骨髄骨形成と吸収2005

    • Author(s)
      杉山稔恵
    • Journal Title

      日本家禽学会誌 第42巻第J4号

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Effects of endocrine disruptors on avian medullary formation2005

    • Author(s)
      T.Sgiyama
    • Journal Title

      Bone Vo.32 Suppl.2

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 内分泌撹乱化学物質DDEのウズラ骨髄骨形成に及ぼす影響2005

    • Author(s)
      坂本尚樹, 杉山稔恵, 西村宏一, 楠原征治
    • Journal Title

      日本畜産学会第104大会講演要旨 (印刷中)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 鳥類の産卵と骨組織2004

    • Author(s)
      杉山稔恵, 楠原征治
    • Journal Title

      THE BONE 18.4

      Pages: 53-58

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Egg laying and bone in birds.2004

    • Author(s)
      T.Sugiyama, S.Kusuhara
    • Journal Title

      THE BONE 18(4)

      Pages: 53-58

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 鳥類の産卵と骨組織2004

    • Author(s)
      杉山稔恵, 楠原征治
    • Journal Title

      THE BONE 18巻4号

    • Related Report
      2004 Annual Research Report
  • [Publications] 前美沙紀, 杉山稔恵, 楠原征治: "ニワトリ卵管におけるダイオキシン受容体の発現と局在"北信越畜産学会報. 第87号(大会号). 29-29 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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