| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2004: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2003: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Research Abstract |
A 1,2-propanediol-producing microorganism, Lactobacillus buchneri, was used as a novel silage starter. The activity to inhibit aerobic deterioration and its related factors were studied. Materials inoculated were whole crop corn, Italian ryegrass, festulolium, whole crop rice and total mixed ration ; changes in microbial flora, fermentation products and aerobic stability were determined. Biogenic amines were also determined in silage to ascertain the safety of L.buchneri in feed preservation. The nutritive value of inoculated silage was evaluated with dairy cows and dry matter intake, milk yield, milk composition and rumen fermentation were determined. The results are as follows.
1.Inoculation of L..buchneri decreased the number of yeasts and improved the silage stability after exposure to air. This benefit was accompanied with reduction of lactic acid and increase of acetic acid and 1,2-propanediol. Wilting diminished the effects of L.buchneri on fermentatio products, while the activit
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y of inhibiting deterioration was kept comparable to that in direct-cut silage. Ensiled total mixed ration did not heat after unloading regardless of L.buchneri inoculation.
2.The three L.buchneri strains of our collection did not produce biogenic amines in a screening media. These characteristics were confirmed in corn, festulolium and TMR silage, thereby non-amine-producing L.buchneri could be considered as a safe silage starter.
3.Benefits of L.buchneri were also found when ensiled in a practical scale silo, but the extents were rather small compared with those in laboratory scale silos. Silages were fed to dairy cows as an ingredient of total mixed ration and no difference was found in dry matter intake, milk yield, milk composition and rumen fluid characteristics. Use of L.buchneri can improve aerobic stability without a decrease in animal performance.
4.Addition of L.buchneri greatly decreased the production of ethanol and 2,3-butanediol in whole crop rice silage. Even after 12 months of ensilage, the L.buchnei added could remain in silage as a dominant microorganism. Less
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