| Project/Area Number |
15580236
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Zootechnical science/Grassland science
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| Research Institution | Kyoto Prefectural University |
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Principal Investigator |
USHIDA Kazunari Kyoto Prefectural University, Graduate School of Agriculture, Professor, 農学研究科, 教授 (50183017)
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| Co-Investigator(Kenkyū-buntansha) |
MIYAZAKI Koji Kyoto Prefectural University, Graduate School of Agriculture, Lecturer, 農学研究科, 講師 (60254322)
KOJIMA Yoichi Kyoto Prefectural University, Graduate School of Agriculture, Professor emeritus, 農学研究科, 教授 (80046490)
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| Project Period (FY) |
2003 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2005: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2004: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2003: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | Lactate-utilizing butyrate producing bacteria / Megasphaera elsdenii / Lactic acid bacteria / non-pathogenic diarrhea / Piglets / Megasphaera elsdenii培養 / ブタ / 下痢 / 酸利用性細菌 |
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| Research Abstract |
Piglets often suffer from non-pathogenic diarrhea most of which have dyspeptic nature. Dyspeptic diarrhea is characterized by the accumulation of lactate in the large intestine. Lactate utilizing butyrate producer, such as Megasphaera elsdenii (ME), can convert lactate into butyrate on which the colonocytes depend to keep their functions. In this study, we used ME as a probiotics to prevent the dyspeptic diarrhea in piglets. We have isolated a couple of ME to select and developed Real-time PCR quantification of ME. The level of ME was 10^3 at 4 days age, and then increased to 10^5 at 13 days old. The level was temporally declined by the supplementation of solid food at 14 days old, then re-increased to 10^6 at 16 days old and stabilized. However, weaning at 25 days old temporally reduced the level of ME. Dyspeptic diarrhea is often caused by the feed change and weaning where the level of ME was decreased. ME was orally administrated using either intestinal soluble or large intestinal soluble capsules to piglets at 3 days, 20 days, and 28 days old for 3 consecutive days. The level of fecal ME was higher shortly after (7 days) dose comparing to the control piglets, but no difference was seen after these days. The level of ME was significantly correlated with that of Lactobacilli. Fecal score was improved with insignificant manner. Clinical condition such as hair score was improved.
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