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Induction of capacitation on boar sperm for a novel relaxin-like protein and its signaling pathway

Research Project

Project/Area Number 15580247
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied animal science
Research InstitutionShizuoka University

Principal Investigator

KOHSAKA Tetsuya  Shizuoka University, Faculty of Agriculture, Professor, 農学部, 教授 (10186611)

Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2004: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 2003: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsRelaxin / RLP / Sperm / Capcitation / Signaling pathways / Boar / 受精能獲得因子 / CTC / cAMP
Research Abstract

This research project was planned to elucidate the physiological significance of relaxin-like protein(RLP) during sperm capacitation. In detail, I will 1) determine whether RLP actually induces boar sperm capacitation and acrosome reaction in vitro, and 2) clarify the intracellular signaling pathways involved in the regulation of RLP-induced capacitation and the acrosome reaction. The outcome of this research is as follows.
1.Induction of boar sperm capacitation and acrosome reaction by RLP
When assessed by Chlortetracycline (CTC) analysis, seminal RLP induced sperm capacitation and acrosome reaction in a dose dependent manner, and the percentage of capacitated sperm was much higher and reached a maximum level (60%) when 150 μg/ml RLP was used. Furthermore, RLP-treated sperm enough high level to fertilizing ability, as assessed by in vitro fertilization test.
2.Intracellular signaling pathways involved in the regulation of RLP-induced capacitation and the acrosome reaction
When investigated the effects of various modulators on boar sperm capacitation, cAMP level and protein tyrosine kinase (PTK) activity induced by RLP, RLP facilitated the influx of HCO_3^-through cholesterol efflux from boar sperm membrane. Also, RLP elevated the cAMP level and PTK activity in the sperm. Thus, the present data suggest that RLP promotes sperm capacitation process via adenylyl cyclase-cAMP-PKA-PTK pathway by facilitation HCO_3^-influx through cholesterol efflux from the sperm membrane.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (8 results)

All 2004 2003

All Journal Article (8 results)

  • [Journal Article] Assessment of bovine X-and Y-bearing spermatozoa in fractions by discontinuous percoll gradients with rapid Fluorescence in situ hybridization2004

    • Author(s)
      J.Kobayashi et al.
    • Journal Title

      J.Reprod.Dev 50

      Pages: 463-469

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Assessment of bovine X-and Y-bearing spermatozoa in fractions by discontinuous percoll gradients with rapid Fluorescence in situ hybridization2004

    • Author(s)
      J.Kobayashi, H.Oguro, H.Uchida, T.Kohsaka, H.Sasada, E.Sato
    • Journal Title

      J.Reprod.Dev 50

      Pages: 463-469

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Assessment of bovine spermatozoa in fraction by discontinuous percoll gradients with rapid fluorescence in situ hybridization2004

    • Author(s)
      J.Kobayashi et al.
    • Journal Title

      J.Reproduction & Development 50

      Pages: 463-469

    • Related Report
      2004 Annual Research Report
  • [Journal Article] ブタ精子の受精能を司る精漿中の鍵分子2003

    • Author(s)
      新井 佳
    • Journal Title

      精子研究 35

      Pages: 11-12

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Seminal immunoreactive relaxin in domestic animals and its relationship to sperm motility as a possible index for predicting fertilizing ability of sires2003

    • Author(s)
      T.Kohsaka et al.
    • Journal Title

      International Journal of Andrology 26

      Pages: 115-120

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Relaxin-like protein as a Key regulatory factor of boar sperm Capacitation (in Japanese)2003

    • Author(s)
      K.ARAI, T.Kohsaka, S.Watanabe, H.Sasada, K.Bamba
    • Journal Title

      Sperm Research 35

      Pages: 11-12

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Seminal immunoreactive relaxin in domestic animals and its relationship to sperm motility as a possible index for Predicting the fertilizing ability of sires2003

    • Author(s)
      T.Kohsaka, K.Hamano, H.Sasada, S.Watanabe, T.Ogine, E.Suzuki, S.Nishida, H.Takahara, E.Sato
    • Journal Title

      International Journal of Andrology 26

      Pages: 115-120

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] ブタ精子の受精能を司る精漿中の鍵分子2003

    • Author(s)
      新井佳他
    • Journal Title

      精子研究会 35

      Pages: 11-12

    • Related Report
      2004 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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