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A role of lymphocyte-derived cytokines in the maintenance and regeneration of sympathetic neurons

Research Project

Project/Area Number 15580263
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Basic veterinary science/Basic zootechnical science
Research InstitutionOsaka Prefecture University

Principal Investigator

KANNAN Yukiko  Osaka Prefecture University, Graduate School of Life & Environmental Sciences, Visiting Researcher, 生命環境科学研究科, 客員研究員 (80264810)

Co-Investigator(Kenkyū-buntansha) MORIYAMA Mitsuaki  Osaka Prefecture University, Graduate School of Life & Environmental Sciences, Associate Professor, 生命環境科学研究科, 助教授 (20275283)
KUWAMURA Mitsuru  Osaka Prefecture University, Graduate School of Life & Environmental Sciences, Assistant Professor, 生命環境科学研究科, 講師 (20244668)
中村 洋一  大阪府立大学, 生命環境科学研究科, 教授 (90180413)
藤本 由香  大阪府立大学, 生命環境科学研究科, 助手 (40405361)
Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2005: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2004: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2003: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsSCID / T lymphocytes / IL-3 / Spleen / Sympathetic neurons / 6-OHDA / Tyrosine hydroxylase / Interleukin 3 / tyrosine hydroxylase / spleen / sympathetic nerves / IL-1 receptor antagonist / lymphocytes
Research Abstract

Immunohistochemical examination revealed that the spleens of immunodeficient (SCID) mice that lack T and B cells had significantly fewer tyrosine hydroxylase (TH)-positive fibers in their periarteriolar lymphatic sheaths (PALS) than their wild type C.B-17 mice. Two weeks after SCID mice were injected with C.B-17 splenic T cells (10^5-10^6 cells), their TH-positive fiber staining increased in the PALS ; there were no significant changes in TH-positive innervation following the injection of SCID mice with C.b-17 cells. T and B cell reconstitution in treated SCID mice was confirmed immunohistochemically using anti-CD3 and anti-IgG antibodies, respectively. IL-3 levels, measured by ELISA, increased significantly in SCID mice following T cell reconstitution. The administration of anti-IL-3 antibody blocked the T cell-induced increase in innervation in the PALS. Anti-Il-3 treatment also inhibited the regeneration of splenic sympathetic neurons in C.B-17 mice after they were chemically sympathetomized with 6-hydroxydopamine (6-OHDA). Our results suggest that T cells play a role in regulating the sympathetic innervation of the spleen, this effect appeared to be mediated at least in part by IL-3.

Report

(4 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (18 results)

All 2007 2006 2005 2004 Other

All Journal Article (14 results) Book (3 results) Publications (1 results)

  • [Journal Article] Potentiation by high potassium of lipopolysaccharide-induced nitric oxide production from cultured astrocytes.2006

    • Author(s)
      Nakamura Y, Moriyama M, Kannan Y, et al.
    • Journal Title

      Neurochem Int 48・1

      Pages: 43-49

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Pyruvate ameliorate the defect in ureogenesis from ammonia in citrin-deficient mice.2006

    • Author(s)
      Moriyama M, Kannan Y, et al.
    • Journal Title

      J Hepatol 44・5

      Pages: 930-938

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Potentiation by high potassium of lipopolysaccharide-induced nitric oxide production from cultured astrocytes.2006

    • Author(s)
      Nakamura Y, et al.
    • Journal Title

      Neurochem Int 48/1

      Pages: 43-49

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Pyruvate ameliorate the defect in ureogenesis from ammonia citrin-deficient mice.2006

    • Author(s)
      Moriyama M, et al.
    • Journal Title

      J Hepatol 44/5

      Pages: 930-938

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Potentiation by high potassium of lipopolysaccharide-induced nitric oxide production from cultured astrocytes.2006

    • Author(s)
      Nakamura Y, Kitagawa T, Ihara H, Kozaki S, Moriyama M, Kannan Y.
    • Journal Title

      Neurochem.Int. 48・1

      Pages: 43-49

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Mutation at the Lmxla locus provokes aberrant brain development in the rat.2005

    • Author(s)
      Kuwamura M, et al.
    • Journal Title

      Brain Res Dev Brain Res 155・2

      Pages: 99-106

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Mutation at the Lmxla locus provokes aberrant brain development in the rat.2005

    • Author(s)
      Kuwamura M, et al.
    • Journal Title

      Brain Res Dev Brain Res 155/2

      Pages: 99-106

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Immunohistochemical and morphometrical studies on myelin breakdown in the demyelination (dmy) mutant rat.2004

    • Author(s)
      kuwamura M, et al.
    • Journal Title

      Brain Res 1022・1-2

      Pages: 110-116

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Beneficial effects of troglitazone on neutrophil dysfunction in multiple low-dose streptozotocin-induced diabetic mice.2004

    • Author(s)
      kannan Y, et al.
    • Journal Title

      Clin Exp Immunol 137・2

      Pages: 264-271

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Cerebral ganglioneuroblastoma in a golden retriever dog2004

    • Author(s)
      Kuwamura M, et al.
    • Journal Title

      Vet Pathol 41・3

      Pages: 282-284

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Immunohistochemical and morphometrical studies on myelin breakdown in the demyelination (dmy) mutant rat.2004

    • Author(s)
      Kuwamura M, et al.
    • Journal Title

      Brain Res 1022/1-2

      Pages: 110-116

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Beneficial effects of troglitazone on neutrophil dysfunction in mutiple low-dose streptozotocin-induced diabetic mice.2004

    • Author(s)
      Kannan Y, et al.
    • Journal Title

      Clin Exp Immunol 137/2

      Pages: 282-284

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Cerebral ganglioneuroblastoma in a golden retriever dog.2004

    • Author(s)
      Kuwamura M, et al.
    • Journal Title

      Vet Pathol 41/3

      Pages: 282-284

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Beneficial effects of troglitazone on neutrophil dysfunction in multiple low-dose streptozotocin-induced diabetic mice.2004

    • Author(s)
      Y.Kannan, M.Tokunaga, M.Moriyama, H.Kinoshita, Y.Nakamura.
    • Journal Title

      Clin.Exp.Immunol. 137

      Pages: 264-271

    • Related Report
      2004 Annual Research Report
  • [Book] Lymphocytes and adrenergic sympathetic nerve system : the role of cytokines in Cytokines and the Brain ed by C.Phelps & E.Korneva.2007

    • Author(s)
      Kannan Y, Moriyama M., et al.
    • Publisher
      Elsevier Science(In press)
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] Neuroendocrine-immune network in stress in The Laboratory Mouse ed by H.Hedrich2004

    • Author(s)
      Kannan Y.
    • Total Pages
      9
    • Publisher
      Elsevier Science
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] Neuroendocrine - immune network in stress in The Laboratory Mouse (ed by H.Hedrich)2004

    • Author(s)
      Y.Kannan
    • Total Pages
      9
    • Publisher
      Elsevier
    • Related Report
      2004 Annual Research Report
  • [Publications] Yukiko Kannan: "LABORATORY MICE"ELSEVIER SCIENCE. 9 (2004)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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