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ANALYSIS OF ADHESIVE AND INVASION MECHANISM OF PIROPLASMA IN VECTOR TICK

Research Project

Project/Area Number 15580265
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Basic veterinary science/Basic zootechnical science
Research InstitutionKITASATO UNIVERSITY

Principal Investigator

OYAMADA Takashi  KITASATO UNIVERSITY, VETERINARY PARASITOLOGY, PROFESSOR, 獣医畜産学部, 教授 (80050665)

Co-Investigator(Kenkyū-buntansha) IKADAI Hiromi  KITASATO UNIVERSITY, VETERINARY PARASITOLOGY, ASSISTANT PROFESSOR, 獣医畜産学部, 講師 (80327460)
YOSHIOKA Kazuki  KITASATO UNIVERSITY, VETERINARY ANATOMY, ASSISTANT PROFESSOR, 獣医畜産学部, 講師 (30327457)
MATSUU Aya  KITASATO UNIVERSITY, VETERINARY INTERNAL MEDICINE I, RESEARCH ASSOCIATE, 獣医畜産学部, 助手 (40348595)
Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2005: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2004: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2003: ¥2,800,000 (Direct Cost: ¥2,800,000)
KeywordsPIROPLASMA / HAEMAPHYSALIS LONGICORNIS / TRANSMISSOIN / BABESIA CABALLI / BABESIA EQUI / BABESIA GIBSONI / ADHESIVE / INVASION / モノクローナル抗体 / 伝播 / 付着および侵入機構 / Lactoferrin / ピロプラズマ原虫感染症 / 付着・侵入機構 / 遺伝子 / 組換え蛋白質 / Loop-mediated Isothermal Amplification Method / 迅速高感度簡易診断法
Research Abstract

1.We investigated the usefulness of the needle injection method as an experimental means of transferring infection of equine Babesia parasites, namely Babesia caballi and Babesia equi, to the tick Haemapysalis longicornis. Further, we also determine investigated the transovarial and transstadial transmission of these parasites in this tick. Engorged adult or nymph ticks were injected with B.caballi- or B.equi-infected erythrocytes into the midgut from the integument by hypodermic needle. Detection of equine Babesia in ticks was by nested PCR for parasite DNA. For transovarial transmission, B.caballi and B.equi DNA were detected in adult ticks, laid eggs and larvae, respectively. For transstadial transmission, B. equi DNA was detected in adult ticks after molt of the injected nymph, and in salivary glands and carcass tissues of adult ticks after molt of the injected nymph. These findings support the potential role of H.longicornis in the transmission of equine Babesia.
2.We established mAbs against the Babesia surface protein and extracellular merozoite termed mAb, and used it to screen cDNA expression library prepared from Babesia merozoite mRNA for highly expressed proteins.
3.Real time-PCR was found to be reproducible and accurate for the quantification of parasite DNA in experimentally infection and far more sensitive than traditional microscopic examination. LAMP method provides a useful tool for the detecton of B.gibsoni infection in dog.
4.Apo-LF had the strongest inhibitory effect on B.caballi ; this may have been caused by inactivation or inhibition of a growth factor in the culture medium.

Report

(4 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (28 results)

All 2006 2005 2004 Other

All Journal Article (27 results) Publications (1 results)

  • [Journal Article] Cloning of the Babesia gibsoni cytochrome b gene and isolation of three single nucleotide polymorphisims trom parasites present after atovaquone treatment.2006

    • Author(s)
      Matsuu, A., Miyamoto, K.(その他3名)
    • Journal Title

      American Journal of Tropical Medicine and Hygine 74・4(In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Inhibitory effect of lactoferrin on in vitro grouth of Babesia caballi.2005

    • Author(s)
      Ikadai, H., Tanaka, T.(その他7名)
    • Journal Title

      Amercan Journao of tropical Medicine and Hygine 73・4

      Pages: 710-712

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Devekioment of a SYBR Green real-time polymerase chain riaction assay for quantitative detection of Babesia gibsoni(Asian genotype) DNA.2005

    • Author(s)
      Matsuu, A., Ono, S.(その他6名)
    • Journal Title

      Journal of Veterinry Diagnostic Investigation 17・6

      Pages: 569-573

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Molecular characterization of a putative protein disulfide isomerase from Babesa caballi.2005

    • Author(s)
      Ikadai, H., Tsukada, R.(その他6名)
    • Journal Title

      Parasitology 131・Pt6

      Pages: 775-782

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Molecular cloning and characterization of a putative binding protein of Babesia caballi.2005

    • Author(s)
      Ikadai, H., Takamatsu, Y.(その他5名)
    • Journal Title

      American journal of Tropocal Medicine and Hygine 73・6

      Pages: 1135-1138

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Inhibitory effect of lactoferrin on in vitro growth of Babesia cuballi.2005

    • Author(s)
      Ikadai, H., Tanaka, T., Shibahara, N., Tanaka, H., Matsuu, A., Kudo, N., Shimazaki, K., Igarashi, I., Oyamada, T.
    • Journal Title

      Am.J.Trop.Med.Hyg. 73

      Pages: 710-712

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Development of a SYBR Green real-time polymerase chain reaction assay for quantitative detection of Babesia gibsoni (Asian genotype) DNA.2005

    • Author(s)
      Matsuu, A., Ono, S., Ikadai, H., Uchide, T., Imamura, S., Onuma, M., Okano, S., Higuchi, S.
    • Journal Title

      J.Vet.Diagn.Invest. 17

      Pages: 569-573

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Molecular characterization of a putative protein disulfide isomerase from Babesia caballi.2005

    • Author(s)
      Ikadai, H., Tsukada, R., Sasaki, M., Takashiro, R., Yokoyama, N., Kudo, N., Igarashi, I., Oyamada, T.
    • Journal Title

      Parasitology 131

      Pages: 775-782

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Molecular cloning and characterization of a putative binding protein of Babesia caballi.2005

    • Author(s)
      Ikadai, H., Takamatsu, Y., Takashiro, R., Segawa, A., Kudo, N., Igarashi, I., Oyamada, T.
    • Journal Title

      Am.J.Trop.Med.Hyg. 73

      Pages: 1135-1138

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Inhibitory effect of lactoferrin on in vitro growth of Babesia caballi.2005

    • Author(s)
      Ikadai, H(その他8名)
    • Journal Title

      Am.J.Trop.Med.Hyg. 73

      Pages: 710-712

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Development of a SYBR Green real-time polymerase chain reaction assay for quantitative detection of Babesia gibsoni (Asian genotype) DNA.2005

    • Author(s)
      Matsuu, A(その他7名)
    • Journal Title

      J.Vet.Diagn.Invest. 17

      Pages: 560-564

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Molecular characterization of a putative protein disulfide isomerase from Babesia caballi.2005

    • Author(s)
      Ikadai, H(その他7名)
    • Journal Title

      Parasitology 131

      Pages: 775-782

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Molecular cloning and characterization of a putative binding protein of Babesia caballi.2005

    • Author(s)
      Ikadai, H(その他6名)
    • Journal Title

      Am.J.Trop.Med.Hyg. 73

      Pages: 1135-1138

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Molecular evidence of infections with Babesia gibsoni parasites in Japan and evaluation of the diagnostic potential of a loop-mediated isothermal amplification method.2004

    • Author(s)
      Ikadai, H., Tanak, H.(その他8名)
    • Journal Title

      Journal of Clinical Microbiology 42・6

      Pages: 2465-2469

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Incidence of canine Babesia gibsoni infecton and subclinical infection among Tosa dog in Aomori Prefecture,Japan2004

    • Author(s)
      Matsuu, A., Kawabe, A.(その他4名)
    • Journal Title

      Journal of Vererinary Medical Science 66・8

      Pages: 893-897

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Efficacy of atovaquone against Babesia gibsoni in vivo and in vitro.2004

    • Author(s)
      Matsuu, A., Koshida, Y.(その他6名)
    • Journal Title

      Veterinary Parasitology 124・1-2

      Pages: 9-18

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Babesia caballi : analysis of chromosomes separated by pulse field get electrophoresis.2004

    • Author(s)
      Ikadai, H., Kudo, N.(その他2名)
    • Journal Title

      Journal of protozoology Research 14

      Pages: 32-36

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Molecular evidence of infections with Babesia gibsoni parasites in Japan and evaluation of the diagnostic potential of a loop-mediated isothermal amplification method.2004

    • Author(s)
      Ikadai, H., Tanaka, H., Shibahara, N., Matsuu, A., Uechi, M., Itoh, N., Oshiro, S., Kudo, N., Igarashi, I., Oyamada, T.
    • Journal Title

      J.Clin.Microbiol. 42

      Pages: 2465-2469

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Incidence of canine Babesia gibsoni infection and subclinical infection among Tosa dog in Aomori Prefecture, Japan.2004

    • Author(s)
      Matsuu, A., Kawabe, A., Koshida, Y., Ikadai, H., Okano, S., Higuchi, S.
    • Journal Title

      J.Vet.Med.Sci. 66

      Pages: 893-897

    • NAID

      110003886481

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Efficacy of atovaquone against Babesia gibsoni in vivo and in vitro.2004

    • Author(s)
      Matsuu, A., Koshida, Y., Kawahara, M., Inoue, K., Ikadai, H., Hikasa, Y., Okano, S., Higuchi, S.
    • Journal Title

      Vet.Parasitol. 124

      Pages: 9-18

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Babesia caballi : analysis of chromosomes separated by pulse field gel electrophoresis.2004

    • Author(s)
      Ikadai, H., Kudo, N., Oyamada, T., Igarashi, I.
    • Journal Title

      J.Protozool.Res. 14

      Pages: 32-36

    • NAID

      130007908530

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Molecular evidence of Babesia gibsoni parasite infections in Japan and evaluation of diagnostic potential in Loop-mediated isothermal amplification method.2004

    • Author(s)
      Ikadai, H (その他9名)
    • Journal Title

      Journal of Clinical Microbiology 42・6

      Pages: 2465-2469

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Incidence of canine Babesia gibsoni infection and subclinical infection among Tosa dog in Aomori Prefecture, Japan.2004

    • Author(s)
      Matsuu, A (その他5名)
    • Journal Title

      Journal of Veterinary Medical Science 66・8

      Pages: 893-897

    • NAID

      110003886481

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Efficacy of atovaquone against Babesia gibsoni in vivo and in vitro.2004

    • Author(s)
      Matsuu, A (その他7名)
    • Journal Title

      Veterinary Parasitology 124・1-2

      Pages: 9-18

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Cloning of the Babesia gibsoni cytochrome b gene and isolation of three single nucleotide polymorphisms from parasites present after atovaquone treatment.

    • Author(s)
      Matsuu, A., Miyamoto, K., Ikadai, H., Okano, S., Higuchi, S.
    • Journal Title

      Am.J.Trop.Med.Hyg. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Babesia caballi : analysis of chromosomes separated by pulse field gel electrophoresis.

    • Author(s)
      Ikadai, H (その他3名)
    • Journal Title

      Journal of Protozoology Research (in press)(掲載予定)

    • NAID

      130007908530

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Inhibitory effect of lactoferrin on the in vitro growth of Babesia caballi protozoan.

    • Author(s)
      Ikadai, H (その他8名)
    • Journal Title

      American Journal of Tropical Medicine and Hygiene (in press)(掲載予定)

    • Related Report
      2004 Annual Research Report
  • [Publications] Ikadai, H., Tanaka H., Shibahara, N., Matsuu, A., Uechi, M., Itoh, N., Oshiro, S., Kudo, N., Igarashi, I., Oyamada, T.: "Molecular Evidence of Babesia gibsoni Parasite Infections in Japan and Evaluation of the Diagnostic Potential of a Loop-mediated Isothermal Amplification Method"Journal of Clinical Microbiology. (accepted)(掲載予定).

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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