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Regulatory mechanism of mast cell function by the TGF-β family

Research Project

Project/Area Number 15580268
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Basic veterinary science/Basic zootechnical science
Research InstitutionAzabu University

Principal Investigator

FUNABA Masayuki  Azabu University, School of Veterinary Medicine, Assistant Professor, 獣医学部, 講師 (40238655)

Co-Investigator(Kenkyū-buntansha) IKEDA Teruo  Azabu University, School of Veterinary Medicine, Associate Professor, 獣医学部, 助教授 (60151297)
MURAKAMI Masaru  Azabu University, School of Veterinary Medicine, Professor, 獣医学部, 教授 (80271360)
Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2005: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2004: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2003: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsTGF-β family / mast cell / signal transduction / cell differentiation / activin A / TGF-β / tocopherol-associated protein / 遊走能 / Smad3 / p38キナーゼ / activin / BMP / MITF / Smad
Research Abstract

The multivalent binding of an antigen to receptor bound IgE and the subsequent aggregation of the high affinity receptor for IgE (FcεRI) provide the trigger for activation of mast cells, leading to synthesis and secretion of various inflammatory molecules including histamine. The previous study indicated gene induction of activin A, a member of the TGF-β family, in mast cells in response to the activation (Cell Signal., 15:605.2003). The present study examined to clarify the regulatory mechanism of mast cell function by the TGF-β family. Mast cells migrated toward TGF-β1 and activin A. These ligands also had activities to inhibit cell proliferation and to stimulate maturation of mast cells. Furthermore, the ligands induced genes related to the maturation (J.Leukoc.Biol., 73:793.2003; Cell.Signal., 17:121.2005, 18:256.2006). To explore the modulation of mast cell function by the TGF-β family at the molecular level, genes induced during the initial phase of treatment with TGF-β1 and activin A were examined in mast cells. As the result, tocopherol-associated protein (Tap) was identified as a TGF-β/activin-inducible gene, which was restricted in mast cells and enhanced TGF-β/activin signaling (in preparation). MITF, a tissue-specific transcription factor, is involved in mast cell maturation. Thus, relationship between MITF and the TGF-β family pathway was further examined. The results suggested that 1)MITF associated with Smad2/3, signal mediators for the TGF-β/activin pathway, 2)MITF negatively regulated Smad2/3 signaling, and 3)expression of Smad2/3 is decreased by the expression of MITF.

Report

(4 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (18 results)

All 2006 2005 2004 2003 Other

All Journal Article (15 results) Publications (3 results)

  • [Journal Article] Transcriptional regulation of plasminogen activator inhibitor-1 by transforming growth factor-β, activin A and microphthalmia-associated transcription factor.2006

    • Author(s)
      Murakami M, Ikeda T, Saito T, Ogawa K, Nishino Y, Nakaya K, Funaba M
    • Journal Title

      Cell. Signal. 18・2

      Pages: 256-265

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Transcriptional regulation of plasminogen activator inhibitor-1 by transforming growth factor-β, activin A and microphthalmia-associated transcription factor.2006

    • Author(s)
      Murakami M, Ikeda T, Saito T, Ogawa K, Nishino Y, Nakaya K, Funaba, M
    • Journal Title

      Cell.Signal. 18(2)

      Pages: 256-265

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Up-Regulation of mouse mast cell protease-6 gene by transforming growth actor-β and activin in mast cell progenitors2005

    • Author(s)
      Funaba M, Ikeda T, Murakami M, Ogawa K, Abe M
    • Journal Title

      Cell. Signal. 17・1

      Pages: 121-128

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Responses during cell preparation for functional analyses in mouse bone marrow-derived cultured mast cells.2005

    • Author(s)
      Murakami M, Ikeda T, Nishino Y, Funaba M
    • Journal Title

      Cell. Immunol. 238・1

      Pages: 49-55

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Up-Regulation of mouse mast cell protease-6 gene by transforming growth factor-β and activin in mast cell progenitors2005

    • Author(s)
      Funaba M, Ikeda T, Murakami M, Ogawa K, Abe M
    • Journal Title

      Cell.Signal. 17(1)

      Pages: 121-128

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Responses during cell preparation for functional analyses in mouse bone marrow-derived cultured mast cells.2005

    • Author(s)
      Murakami M, Ikeda T, Nishino Y, Funaba, M
    • Journal Title

      Cell.Immunol. 238(1)

      Pages: 49-55

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Up-regulation of mouse mast cell protease-6 gene by transforming growth factor-beta and activin in mast cell progenitors.2005

    • Author(s)
      Funaba M, Ikeda T, Murakami M, Ogawa K, Abe M.
    • Journal Title

      Cell.Signal. 17・1

      Pages: 121-128

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Reliability of RT-PCR methods for measuring relative gene expression in mast cells2004

    • Author(s)
      Ikeda T, Murakami M, Funaba M
    • Journal Title

      Vet. Immunol. Immunopathol. 100・1-2

      Pages: 1-5

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Expression of tocopherol-associated protein in mast cells.2004

    • Author(s)
      Ikeda T, Murakami M, Funaba M
    • Journal Title

      Curt. Diag. Lab. Immunol. 11・6

      Pages: 1189-1191

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Reliability of RT-PCR methods for measuring relative gene expression in mast cells.2004

    • Author(s)
      Ikeda T, Murakami M, Funaba, M
    • Journal Title

      Vet.Immunol.Immunopathol. 100(1-2)

      Pages: 1-5

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Expression of tocopherol-associated protein in mast cells.2004

    • Author(s)
      Ikeda T, Murakami M, Funaba, M
    • Journal Title

      Clin.Diag.Lab.Immunol. 11(6)

      Pages: 1189-1191

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Reliability of RT-PCR methods for measuring relative gene expression in mast cells.2004

    • Author(s)
      Ikeda, T., Murakami, M., Funaba, M.
    • Journal Title

      Vet.Immunol.Immunopathol. 100・1

      Pages: 1-5

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Expression of tocopherol-associated protein in mast cells.2004

    • Author(s)
      Ikeda, T., Murakami, M., Funaba, M.
    • Journal Title

      Clin.Diag.Lab.Immunol. 11・6

      Pages: 1189-1191

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Transcriptional activation of mouse mast cell protease-7 by activin and transforming growth factor-β is inhibited by microphthalmia-associated transcription factor.2003

    • Author(s)
      Funaba M, Ikeda T, Murakami M, Ogawa K, Tsuchida K, Sugino H, Abe M
    • Journal Title

      J. Biol. Chem. 278・52

      Pages: 52032-52041

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Transcriptional activation of mouse mast cell protease-7 by activin and transforming growth factor-β is inhibited by microphthalmia-associated transcription factor.2003

    • Author(s)
      Funaba M, Ikeda T, Murakami M, Ogawa K, Tsuchida K, Sugino H, Abe M.
    • Journal Title

      J.Biol.Chem. 278(52)

      Pages: 52032-52041

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Publications] Funaba, M., Ikeda T., Ogawa, K., Murakami, M., Abe, M.: "Role of activin A in murine mast cells : modulation of cell growth, differentiation and migration."J.Leukoc.Biol.. 73・6. 793-801 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Murakami, M., Ikeda, T., Ogawa, K., Funaba, M.: "Transcriptional activation of mouse mast cell protease 9 by microphthalmia-associated transcription factor."Biochem.Biophys.Res.Commun.. 311・1. 4-10 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Funaba, M., Ikeda, T., Murakami, M., Ogawa, K., Tsuchida, K., Sugino, H., Abe, M.: "Transcriptional activation of mouse mast cell protease-7 by activin and transforming growth factor-β is inhibited by microphthalmia-associated transcription factor."J.Biol.Chem.. 278・52. 52032-52041 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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