| Project/Area Number |
15580271
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied veterinary science
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| Research Institution | Iwate University |
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Principal Investigator |
TSUDA Shuji Iwate University, Faculty of Agriculture, Professor, 農学部, 教授 (60281953)
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| Co-Investigator(Kenkyū-buntansha) |
SATO Itqaru Iwate University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (60225919)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2004: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2003: ¥2,700,000 (Direct Cost: ¥2,700,000)
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| Keywords | DNA damage / comet method / food additives / food red 2 / carcinogenicity / DNAの損傷 |
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| Research Abstract |
The data concerning to the species specific carcinogenicity of food red 2 were as follows :
1.DNA damage in colon mucous membrane of mice induced by food red 2 by ip injection was less prominent than po administration.
2.That DNA damage in colon disappeared when the bile legation was applied before po administration.
3.That DNA damage in colon was observed where the food red 2 reached.
4.Colon strips Isolated from either rats or mice showed DNA damage, when incubated with colon content of mice after oral administration of food red 2.
From these results, the difference between rats and mice in DNA damage of colon is suggested to be due to the difference in food red 2 metabolism between the two species.
Using HPLC we identified a specific peak in the colon content of mice after oral administration of food red 2.
After the 5 days multiple administration of food red 2, colon DNA damage in mice disappeared, which may be the decrease of sensitivity of cells in colon mucous membrane.
In order to more precisely extrapolate rodent data to humans concerning the carcinogenicity of food red 2, identification of food red 2 metabolite in rats would be necessarily.
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