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Investigation for the infectious cycle of rotaviruses in nature and prediction of the epidemics of novel rotaviruses

Research Project

Project/Area Number 15580273
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied veterinary science
Research InstitutionGifu University

Principal Investigator

SUGIYAMA Makoto  Gifu University, United Graduate School of Veterinary Sciences, Professor, 連合獣医学研究科, 教授 (80196774)

Co-Investigator(Kenkyū-buntansha) MINAMOTO Nobuyuki  Gifu University, Faculty of Applied Biological Sciences, Professor, 応用生物科学部, 教授 (10144007)
KITAGAWA Hitoshi  Gifu University, Faculty of Applied Biological Sciences, Professor, 応用生物科学部, 教授 (70144003)
Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2005: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2004: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2003: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsGroup A rotavirus / Seroepidemiological study / Purified VP8 expressed in E.coli / Latex agglutination test / P genotype / Zoonosis / Infectious cycle / Japanese encephalitis virus / ロタウイルス / 血清疫学 / 中和試験
Research Abstract

The latex agglutination (LA) tests for detection of P genotype-specific antibody to Group A rotavirus were established. The antibodies to P[2] and [3] rotaviruses, which were epidemiologically relevant in some animal species, were detected by the LA test using the purified VP8 of simian rotavirus SA-11 strain as a antigen (SA-LA test). Likewise, the antibodies to the most frequent P[4] and [8] rotaviruses in human and p[17] rotavirus in birds were detectable in the LA tests using the VP8s of human rotavirus Wa strain (Wa-LA test) and pigeon rotavirus PO-13 strain (PO-LA test), respectively. The seroepidemiological study on rotavirus infection in human, domestic and wild animals was performed by using the LA tests described above. The results in three tests suggested that these human and animals should intricately suffer the infection of various rotaviruses. Interestingly, the investigation by PO-LA test showed that avian p[17] rotavirus was prevalent in human, suggesting that avian rotavirus may be a zoonotic agent. From the results in three LA tests, it was indicated that P[2] or [3], [4] or [8], and [17] rotavirus infections would be highly frequent in cattle and wild boar and these animals might be infected with an unknown P genotype rotavirus. The results in this study provide the important information to prevent human and animals from the rotavirus infection. In the sera collected in this study, the epidemiological investigation on Japanese encephalitis virus was also performed by using neutralizing antibody test. This result suggests that wild boar may play a role as an amplifier in the infectious cycle of Japanese encephalitis viruses in Japan.

Report

(4 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (6 results)

All 2006 2004 2000 Other

All Journal Article (5 results) Publications (1 results)

  • [Journal Article] 国内の野生動物における日本脳炎ウイルスに対する血清疫学調査-イノシシが日本脳炎ウイルスの増幅動物である可能性-2006

    • Author(s)
      杉山誠, 高木愛香, 源宣之, 伊藤直人, 浅野玄, 坪田敏男, 石黒直隆, 伊藤雅, 山下照夫, 榮賢司
    • Journal Title

      獣医畜産新報 59・4

      Pages: 284-285

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Seroepidemiological study on Japanese encephalitis in wild animals of Japan : Wild boar may play a role as an amplifier in the infectious cycle of Japanese encephalitis virus2006

    • Author(s)
      M.Sugiyama, A.Takagi, N.Minamoto, N.Ito, M.Asano, T.Tsubota, N.Ishiguro, M.Ito, T.Yamashita, K.Sakae.
    • Journal Title

      Journal of Veterinary Medicine 59-4

      Pages: 284-285

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Attachment and infection to MA104 cells of avian rotaviruses require the presence of sialic Acid on the cell surface2004

    • Author(s)
      M.Sugiyama, K.Goto, H.Uemukai, Y.Mori, N.Ito, N.Minamoto
    • Journal Title

      Journal of Veterinary Medical Science 66・4

      Pages: 461-463

    • NAID

      110003886430

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Attachment and infection to MA104 cells of avian rotaviruses require the presence of sialic Acid on the cell surface2004

    • Author(s)
      M.Sugiyama, K.Goto, H.Uemukai, Y.Mori, N.Ito, N.Minamoto.
    • Journal Title

      Journal of Veterinary Medical Science 66-4

      Pages: 461-463

    • NAID

      110003886430

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 国内の野生動物における日本脳炎ウイルスに対する血清疫学調査-イノシシが日本脳炎ウイルスの増幅動物である可能性-2000

    • Author(s)
      杉山誠, 高木愛香, 源宣之, 伊藤直人, 淺野玄, 坪田敏男, 石黒直隆, 伊藤雅, 山下照夫, 榮賢司
    • Journal Title

      獣医畜産新報 59・4

      Pages: 3-3

    • Related Report
      2005 Annual Research Report
  • [Publications] M.Sugiyama, K.Goto, H.Uemukai, Y.Mori, N.Ito, N.Minamoto: "Attachment and infection to MA104 cells of avian rotaviruses require the presence of sialic Acid on the cell surface"Journal of Veterinary Medical Science. (印刷中).

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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