Development of recycling biomass for recycling systems of organic waste
| Project/Area Number |
15580297
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Boundary agriculture
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| Research Institution | Okayama University |
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Principal Investigator |
SUGIMOTO Manabu Okayama University, Research Institute for Bioresources, Associate Professor, 資源生物科学研究所, 助教授 (20216336)
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| Project Period (FY) |
2003 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2005: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2004: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2003: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | serine protease / earthworm / transgenic plant / biomass / organic waste / 活性型 / 前駆体 / コムギ胚芽無細胞発現 |
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| Research Abstract |
The genes encoding pro-form and active-form of strong fibrinolytic protease, isozme A, in the six isozymes of eartworm, Lumbricus rubellus, were subcloned into a plasmid, pEU3-NII, and expressed in the wheat germ cell-free protein synthesis system. Extra proteins with a molecular mass of about 26 and 25 kDa were produced by pro-form and active-form of plasmids, respectively, which are similar to calculated molecular mass from the amino acid sequences. These results shows that the gene from earthworm could express in plant.
The pro-form and active-form genes of isozyme A were subcloned into a plasmid pBI121, resulting the plasmids both of which the enzyme genes fused with CaMV35S promoter. These genes were transferred to Arabidopsis thaliana, cv. Columbia, and T1 seeds were obtained. T1 seeds including active-form gene were cultured on MS medium agar plate containing kanamycine and three types of plants, 1) normal shape, 2) breached leaf, 3) wrinkled leaf, were observed. All types of transgenic plants expressed extra isozyme A mRNA, but could not produce T2 seeds. The transgenic plants of T1 seeds including pro-form gene also showed the wrinkled leaves which is breached gradually, resulting the die. These results suggest that the isozyme A from earthworm is produced and works in plants, and the possibility of producing the organic waste-degrading plants if the expression of the isozyme A gene could be controlled by adequate promoters which can restrict the expression strongly or organ-specific expression affecting little on plant growth.
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Report
(4 results)
Research Products
(7 results)
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[Journal Article] An isozyme of earthworm serine protease acts on hydrolysis of triacylglycerol2005
Author(s)
Nakajima, N., Sugimoto, M., Tsuboi, S., Tsuji, H., Ishihara, K.
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Journal Title
Bioscience, Biotechnology, and Biochemistry 69
Pages: 2009-2011
NAID
Related Report
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