| Project/Area Number |
15580309
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Tokyo University of Science |
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Principal Investigator |
KAMAKURA Takashi Tokyo University of Science, Dept.of Applied.Biological Science, Associate Professor, 理工学部, 助教授 (70177559)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2004: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2003: ¥2,600,000 (Direct Cost: ¥2,600,000)
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| Keywords | Plant pathogenic fungi / Infection specific structure / Regulation of gene expression / Magnaporthe grisea / Appressorium formation / Germ tube / Lysophospholipid |
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| Research Abstract |
The aim of this research project is clarify the function of genes organizing the infection stage of the plant pathogenic fungi, Magnaporthe grisea, especially the mechanism of development of the infection specific structure, we have further analyzed the gene function of CBPl which may play an important role for induction of the development of appressorium of rice blast pathogen Magnaporthe grisea. The CBPl gene is specifically expressed in germ tube, but completely repressed in vegetative hyphae. This expression pattern may be under the general regulation system during infectious stage of this fungi. We estimated the promoter control region of the gene by using a reporter gene EGFP and succeeded to encompass the critical control region within less than 50 base pairs, and found some candidates of meaningful base sequences in there. We have analysed another gene LPLl, which can code lysophospholipase. The knockout mutants of LPLl(lpll) showed imcomplete development of the function of appressorium (an infection specific structure). It is known to be important to generate turgor pressure in appressoriumm to penetrate into the host plant. The lpll mutant showed significant delay of this process. The turgor pressure has to be thought to be generated by glycerol which is produced by the degradation of glycogen. In this study, we found the contribution of the lipid metabolism is also an important factor for the turgor generation of appressorium.
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